FastQCFastQC Report
Thu 26 May 2016
SRR1512071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35000.2965513618485826No Hit
GGTATCAACGCAGAGTACTTTTTTT22460.19030124534626186No Hit
TATCAACGCAGAGTACTTTTTTTTT20580.17437220076696655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG903.3757715E-713.1664831
CTCAACG450.00890837213.1664832
GGTATCA8000.012.837321
TGGCGAG5450.010.93282518
CTATACC700.001547395710.808874
GCATTAA650.00798655410.6344661
AACAGTG2600.010.5490427
GGCGAGG3150.010.50862419
GGTCTAT854.6144528E-410.4557361
GTCCTAT1156.468208E-610.3042031
GTAGAAC2500.010.2143823
TGTGCGC750.002746408810.0882810
TAGACTG1606.6265784E-910.0488725
AGACAAT1054.307711E-59.9081326
GGACGTG5400.09.6329056
ATGGCGA6100.09.61280617
TAGAACA3450.09.59483054
GTCCTAC5900.09.5401211
TATGGCG5750.09.54000416
GTATAGA1251.7417173E-59.4798671
Produced by FastQC (version 0.11.5)