FastQCFastQC Report
Thu 26 May 2016
SRR1512071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41210.34916804633657395No Hit
GGTATCAACGCAGAGTACTTTTTTT28580.2421553691894997No Hit
TATCAACGCAGAGTACTTTTTTTTT26600.22537903500492273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12940.10963927492344738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.012.2903111
TGCGCCA550.003076467412.08629912
CTAAGAC1209.962605E-911.878023
GCCTAAC600.005695005411.12955951
CGTGCGC600.005890937511.07910710
TAGGACA4000.010.6902194
CCTACAC1252.2295353E-710.6427073
AGGACCT5500.010.3662725
AAGGACC3400.010.3408654
CTAGGAC1752.3464963E-1010.316913
GGACCTG5200.010.2268696
GTCCTAC5800.010.1975271
TTAGAAC1409.638097E-810.181163
ATTATTC1502.4308974E-810.135913
GTAGCAC951.6388489E-410.0025433
TAGGACT1356.411792E-79.8543584
TAGGACC2900.09.8300864
GTAGGAC7300.09.7627573
TATACTG1951.6189006E-109.7460685
TAAGACA2054.1836756E-119.7341824
Produced by FastQC (version 0.11.5)