FastQCFastQC Report
Thu 26 May 2016
SRR1512070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466939
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39720.2707679051412499No Hit
GGTATCAACGCAGAGTACTTTTTTT25020.1705592393412405No Hit
TATCAACGCAGAGTACTTTTTTTTT24980.17028656269960782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.013.2925091
GGTCTAG1052.2116328E-611.2710691
GGCGAGG3500.011.08069619
GTATCAA14800.010.7284571
GTCCTAC5550.010.3064261
TGGCGAG7250.010.0462518
CTAGGAC2450.010.0382623
TTAGGAC5050.09.9274053
CTACTAT1201.09056655E-59.8621851
TATGGCG7800.09.82294416
TAGGACA4550.09.7709714
ATGGCGA8250.09.74577117
GTGTAGA2251.2732926E-119.6430241
AGGACGT6550.09.5313385
GGACGTG6200.09.4591316
GTATTAA2103.274181E-109.3925571
GTACAAT1053.0875267E-49.3925571
GAGTAAT1053.0875267E-49.3925571
TAGGACG7300.09.3295554
GCTAATA850.0054665099.2820552
Produced by FastQC (version 0.11.5)