##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1466939 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.48227704083128 31.0 30.0 34.0 25.0 34.0 2 26.959264836506495 31.0 26.0 34.0 10.0 34.0 3 28.641755383148176 31.0 28.0 34.0 16.0 34.0 4 32.98304019458205 35.0 33.0 37.0 27.0 37.0 5 33.384137990741266 35.0 35.0 37.0 28.0 37.0 6 33.64370433944424 37.0 35.0 37.0 28.0 37.0 7 33.707756764255365 37.0 35.0 37.0 29.0 37.0 8 33.750777639697354 37.0 35.0 37.0 29.0 37.0 9 35.361451294157426 39.0 35.0 39.0 30.0 39.0 10 35.23928056994872 39.0 35.0 39.0 30.0 39.0 11 35.25726154938958 39.0 35.0 39.0 30.0 39.0 12 35.16917949553458 39.0 35.0 39.0 29.0 39.0 13 35.15565268903479 39.0 35.0 39.0 29.0 39.0 14 36.29250227855419 40.0 36.0 41.0 29.0 41.0 15 36.226836971407806 40.0 36.0 41.0 29.0 41.0 16 36.19948477748564 40.0 36.0 41.0 29.0 41.0 17 36.14616967713041 40.0 36.0 41.0 29.0 41.0 18 36.097993168086745 40.0 36.0 41.0 28.0 41.0 19 36.12270585211791 40.0 36.0 41.0 28.0 41.0 20 36.05670515270233 40.0 36.0 41.0 28.0 41.0 21 35.987707055303595 40.0 36.0 41.0 27.0 41.0 22 35.88841594640268 40.0 36.0 41.0 27.0 41.0 23 35.79192386322812 40.0 36.0 41.0 27.0 41.0 24 35.69374186656705 40.0 36.0 41.0 27.0 41.0 25 35.57689583547782 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5848997669018914 1101 2 3.0291455076799494 1101 3 1.4876023046094318 1101 4 0.6841977872954246 1101 5 0.38598750631673795 1101 6 -0.05027586174369958 1101 7 -0.19644439014135884 1101 8 -0.21229174975355392 1101 9 -0.2188649425885032 1101 10 -0.41290029950125273 1101 11 -0.16048453965095177 1101 12 -0.2147470063734147 1101 13 -0.2954113727804426 1101 14 -0.1939390213086014 1101 15 -0.21409737964814468 1101 16 -0.2102797478799303 1101 17 -0.23964117974674082 1101 18 -0.3119600500271673 1101 19 -0.3693925113260903 1101 20 -0.43629874408611613 1101 21 -0.24458223894485087 1101 22 -0.40160961206350265 1101 23 -0.47077146399923464 1101 24 -0.47159154593408914 1101 25 -0.5146006914904078 1102 1 1.0788575209516225 1102 2 3.6304999529281083 1102 3 2.0987849390419377 1102 4 1.2264371013794033 1102 5 0.9833469685203511 1102 6 0.6835733243282789 1102 7 0.5353210340103658 1102 8 0.562831185563553 1102 9 0.6587538617037794 1102 10 0.43234719699815827 1102 11 0.5694459794977575 1102 12 0.4509419291162331 1102 13 0.5644556380829897 1102 14 0.42444719135456666 1102 15 0.4003436213539189 1102 16 0.44616498265779825 1102 17 0.18608332569161234 1102 18 0.36532229971302854 1102 19 0.3285150997138615 1102 20 0.4243082013445445 1102 21 0.5111099482751911 1102 22 0.6103421561478513 1102 23 0.4166124857250253 1102 24 0.12392305698981687 1102 25 0.21608635268667342 1103 1 1.0675954463826969 1103 2 3.0551314287015927 1103 3 2.005338415357084 1103 4 1.4359445750378157 1103 5 1.08940649985297 1103 6 0.9727987463739609 1103 7 0.8703653342979649 1103 8 0.6765288086951387 1103 9 0.8841759073840194 1103 10 0.7435647778284533 1103 11 0.7315482764064072 1103 12 0.5061464068629178 1103 13 0.609555523302042 1103 14 0.7429868939558375 1103 15 0.6604675589853102 1103 16 0.6844354014148095 1103 17 0.8059584852887198 1103 18 0.7889349333554563 1103 19 0.9413067271529627 1103 20 0.9519330095055949 1103 21 1.0129348363960062 1103 22 0.8665648311887963 1103 23 0.9136526848506321 1103 24 0.7436885943323119 1103 25 0.8162552999261266 1104 1 1.42745400455955 1104 2 4.078275061115118 1104 3 2.49685506113952 1104 4 1.7854780604203597 1104 5 1.3414789966752636 1104 6 0.9629406389358692 1104 7 1.038795591489503 1104 8 0.9831934320127615 1104 9 1.0154616847731575 1104 10 1.16842302048817 1104 11 1.024579404425232 1104 12 1.1814260902640115 1104 13 0.9323710178976867 1104 14 0.9760895992898782 1104 15 0.8392914012627983 1104 16 0.8813293606139396 1104 17 0.9801789326018664 1104 18 1.0099636995031673 1104 19 0.9762376289009254 1104 20 0.892371488172131 1104 21 1.0196832971231373 1104 22 0.9130815220451112 1104 23 0.9646438593856956 1104 24 1.087593254069624 1104 25 1.184037394779594 1105 1 0.854432822842714 1105 2 3.5140389874645983 1105 3 1.9202497998743695 1105 4 1.250717928079581 1105 5 0.7924486266245552 1105 6 0.45703924719997246 1105 7 0.1121305431203865 1105 8 0.36224816643215263 1105 9 0.31092865352203347 1105 10 0.26242183373663863 1105 11 0.10186390113469201 1105 12 0.0513671123088244 1105 13 -0.03564018431758598 1105 14 0.1528081413835949 1105 15 0.2327040311761337 1105 16 0.170653991102661 1105 17 0.2617159556536137 1105 18 0.15958205099740042 1105 19 0.44649287038688357 1105 20 0.4223522484123592 1105 21 0.36376302524914905 1105 22 0.4585840034346873 1105 23 0.24710376156859581 1105 24 0.4414250230486587 1105 25 0.4281404983146757 1106 1 0.5650325439026886 1106 2 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1108 25 0.43435216951892386 1109 1 0.9383051816180483 1109 2 3.4217187089797108 1109 3 1.8394368451803302 1109 4 1.3401700695754997 1109 5 0.7724360029964643 1109 6 0.5905367224743543 1109 7 0.47067114043012737 1109 8 0.2686577005750621 1109 9 0.24551183036410862 1109 10 0.3922834954651364 1109 11 0.32434230534118313 1109 12 0.3799952751710123 1109 13 0.35192693304389877 1109 14 0.3103331104331062 1109 15 0.1291363782667858 1109 16 0.31033808741781854 1109 17 0.42212297202607374 1109 18 0.2689511825752504 1109 19 0.34015097524297744 1109 20 0.282948097568692 1109 21 0.4388736124043433 1109 22 0.40429457807058355 1109 23 0.264374310609476 1109 24 0.37085094356501 1109 25 0.4058544044755621 1110 1 0.1145484297791306 1110 2 1.5935666668314532 1110 3 0.7929643356855891 1110 4 0.3970782778009223 1110 5 0.3718804848299513 1110 6 0.3968739168500477 1110 7 0.26720284925087157 1110 8 0.21981389456938416 1110 9 0.09541391260173526 1110 10 0.047406916712581904 1110 11 -0.06514979352211014 1110 12 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-1.0423575186172584 2315 18 -1.2492653906798452 2315 19 -1.1450517890140617 2315 20 -1.1057057752523107 2315 21 -1.2015026551465269 2315 22 -1.168924531602734 2315 23 -1.055097658019676 2315 24 -0.966338213554252 2315 25 -1.0007141288427945 2316 1 -1.0669817154676977 2316 2 -1.1096129655531257 2316 3 -0.627325299941841 2316 4 -1.2401428151022849 2316 5 -1.0776780713072966 2316 6 -1.162983145431582 2316 7 -1.2797484310435863 2316 8 -1.3553855027906607 2316 9 -1.6172406432225799 2316 10 -1.3752057092001806 2316 11 -1.7456496717575476 2316 12 -1.5542004141503938 2316 13 -1.8002680124435742 2316 14 -2.0978771048297205 2316 15 -2.171723266709243 2316 16 -1.9689355477358745 2316 17 -1.8817975749883757 2316 18 -1.760756426303658 2316 19 -1.86553181432064 2316 20 -1.8851160110886696 2316 21 -1.971775242913175 2316 22 -1.86824939327402 2316 23 -1.8803005416191851 2316 24 -1.9517980371368182 2316 25 -1.9507940560508317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48301.0 3 3.0 4 387.0 5 1944.0 6 2361.0 7 2099.0 8 2013.0 9 1874.0 10 1840.0 11 1727.0 12 1848.0 13 1655.0 14 1742.0 15 1866.0 16 1939.0 17 2021.0 18 2438.0 19 3023.0 20 3795.0 21 4949.0 22 6071.0 23 7311.0 24 8474.0 25 10020.0 26 11050.0 27 12951.0 28 15534.0 29 19166.0 30 24165.0 31 32467.0 32 42996.0 33 58628.0 34 81055.0 35 114565.0 36 170986.0 37 241998.0 38 521677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13106339118396 19.435913831454478 13.319640421312679 25.113382356048884 2 16.196751651750684 21.944474453301797 38.92666056381273 22.93211333113479 3 17.94948528875434 24.85174911840233 30.603317520360424 26.595448072482903 4 12.41230889627994 17.458053811371844 38.55674980350239 31.57288748884582 5 13.24404082242002 37.53802987036271 36.05296471087073 13.164964596346543 6 30.942868108353515 37.462498440630455 18.54201163102215 13.05262181999388 7 27.09703675476622 32.19377220184343 22.972802550071954 17.7363884933184 8 24.738297344389515 36.10412752602029 21.40746810349764 17.75010702609255 9 25.82056922612324 15.741486183133723 21.00939439199585 37.42855019874718 10 14.810568128599758 28.875297473173735 34.86886639458082 21.445268003645687 11 33.11262431498515 23.1784689070234 25.024148925074595 18.684757852916857 12 22.4149743104519 25.255037871377066 31.890555776347888 20.439432041823142 13 27.931222770680993 22.263570605185357 26.445066904622482 23.36013971951117 14 20.881906832954424 22.1438577714759 28.328180866909573 28.6460545286601 15 22.69930787851438 30.23574940743957 24.340275907859837 22.724666806186214 16 22.09096629103187 28.53083870563125 27.43358789970135 21.94460710363553 17 21.507589277801788 28.457099073035124 28.124842358709095 21.910469290453992 18 21.294205144181184 27.937835179240583 29.497136554417054 21.270823122161183 19 22.692924625732736 27.474806279496068 28.39563906463349 21.43663003013771 20 22.86983012903758 27.43819264221981 28.619633972011066 21.072343256731543 21 22.69291544700594 27.140615349796654 28.010795275601286 22.155673927596123 22 22.580469239437274 27.656931803695755 28.15422989692057 21.608369059946405 23 22.173427852919744 27.504231635248377 28.241282626879183 22.081057884952692 24 22.27095579689052 27.112417540542072 28.70669621584626 21.90993044672115 25 22.30769125893528 27.37504925225197 28.400723138946564 21.916536349866185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 793.0 1 793.0 2 716.0 3 639.0 4 639.0 5 639.0 6 1359.0 7 2079.0 8 2079.0 9 2079.0 10 3098.0 11 4117.0 12 4117.0 13 4117.0 14 6573.0 15 9029.0 16 9029.0 17 9029.0 18 15080.0 19 21131.0 20 21131.0 21 21131.0 22 31562.5 23 41994.0 24 41994.0 25 41994.0 26 58020.5 27 74047.0 28 74047.0 29 74047.0 30 92728.0 31 111409.0 32 111409.0 33 111409.0 34 126442.0 35 141475.0 36 141475.0 37 141475.0 38 153529.0 39 165583.0 40 165583.0 41 165583.0 42 173593.0 43 181603.0 44 181603.0 45 181603.0 46 183757.0 47 185911.0 48 185911.0 49 185911.0 50 180213.0 51 174515.0 52 174515.0 53 174515.0 54 157522.0 55 140529.0 56 140529.0 57 140529.0 58 119584.0 59 98639.0 60 98639.0 61 98639.0 62 79621.0 63 60603.0 64 60603.0 65 60603.0 66 46037.5 67 31472.0 68 31472.0 69 31472.0 70 22629.5 71 13787.0 72 13787.0 73 13787.0 74 9450.5 75 5114.0 76 5114.0 77 5114.0 78 3429.0 79 1744.0 80 1744.0 81 1744.0 82 1132.0 83 520.0 84 520.0 85 520.0 86 332.0 87 144.0 88 144.0 89 144.0 90 93.5 91 43.0 92 43.0 93 43.0 94 30.5 95 18.0 96 18.0 97 18.0 98 9.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.086536658988547 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.7718412285718766E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.7267664163267865E-4 15 0.0 16 0.0 17 6.816916040816966E-5 18 0.0 19 4.09014962449018E-4 20 1.3633832081633932E-4 21 6.816916040816966E-5 22 8.18029924898036E-4 23 8.18029924898036E-4 24 4.09014962449018E-4 25 6.816916040816966E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1466939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.22563934148864 #Duplication Level Percentage of deduplicated Percentage of total 1 85.2941791848993 66.72191698846241 2 10.600494990003389 16.584609958585247 3 2.411778181216333 5.659886705265009 4 0.7735888950874961 2.420579436227806 5 0.3428330777252738 1.3409168346234905 6 0.17204508422659004 0.807500202551116 7 0.09685741490397551 0.530371324407912 8 0.06756203996813073 0.4228067017377788 9 0.04164600206344628 0.2932006623687036 >10 0.180964134498513 2.3288440535283756 >50 0.009458801786253376 0.5125613677501597 >100 0.007364836731477626 1.1314774025128729 >500 6.136784448568193E-4 0.3366549330335221 >1k 6.136784448568193E-4 0.9086734289456444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3972 0.2707679051412499 No Hit GGTATCAACGCAGAGTACTTTTTTT 2502 0.1705592393412405 No Hit TATCAACGCAGAGTACTTTTTTTTT 2498 0.17028656269960782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.816916040816966E-5 0.0 2 0.0 0.0 0.0 6.816916040816966E-5 0.0 3 0.0 0.0 0.0 6.816916040816966E-5 0.0 4 0.0 0.0 0.0 6.816916040816966E-5 0.0 5 0.0 0.0 0.0 6.816916040816966E-5 0.0 6 0.0 0.0 0.0 6.816916040816966E-5 0.0 7 0.0 0.0 0.0 6.816916040816966E-5 0.0 8 0.0 0.0 0.0 6.816916040816966E-5 0.0 9 0.0 0.0 0.0 1.3633832081633932E-4 0.0 10 0.0 0.0 0.0 2.0450748122450898E-4 0.0 11 0.0 0.0 0.0 3.4084580204084833E-4 0.0 12 0.0 0.0 0.0 3.4084580204084833E-4 0.0 13 0.0 0.0 0.0 3.4084580204084833E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 690 0.0 13.292509 1 GGTCTAG 105 2.2116328E-6 11.271069 1 GGCGAGG 350 0.0 11.080696 19 GTATCAA 1480 0.0 10.728457 1 GTCCTAC 555 0.0 10.306426 1 TGGCGAG 725 0.0 10.04625 18 CTAGGAC 245 0.0 10.038262 3 TTAGGAC 505 0.0 9.927405 3 CTACTAT 120 1.09056655E-5 9.862185 1 TATGGCG 780 0.0 9.822944 16 TAGGACA 455 0.0 9.770971 4 ATGGCGA 825 0.0 9.745771 17 GTGTAGA 225 1.2732926E-11 9.643024 1 AGGACGT 655 0.0 9.531338 5 GGACGTG 620 0.0 9.459131 6 GTATTAA 210 3.274181E-10 9.392557 1 GTACAAT 105 3.0875267E-4 9.392557 1 GAGTAAT 105 3.0875267E-4 9.392557 1 TAGGACG 730 0.0 9.329555 4 GCTAATA 85 0.005466509 9.282055 2 >>END_MODULE