FastQCFastQC Report
Thu 26 May 2016
SRR1512070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466939
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47820.3259849250718673No Hit
TATCAACGCAGAGTACTTTTTTTTT33920.23122979210451153No Hit
GGTATCAACGCAGAGTACTTTTTTT33860.2308207771420625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15940.10866164169062245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG456.754008E-414.7800865
TACACCG400.005273449714.2522255
GGTATCA8700.013.4786371
TGTACCG500.001497714113.3020775
TAGGACC2800.011.8768544
ACCGTGC658.039993E-411.689358
CGTGCGC658.0445927E-411.68855310
GTCCTAG1701.0913936E-1111.2160821
AATCCCG803.7792264E-410.68479619
GTCTAAT750.002570832810.1692481
TAAGTCC856.5830146E-410.0603944
TGGCGAG6900.010.04818318
GTGCGCC1151.0299369E-59.9098611
CTACACT1451.6122249E-79.8291214
GTGTAAG1856.0754246E-109.7913361
GTATCAA18700.09.7885811
GGCGAGG3400.09.776937519
GTAGGAC7000.09.7729543
TTAGGAC5300.09.6807573
GTGGTAT3550.09.6679471
Produced by FastQC (version 0.11.5)