##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512070_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1466939 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.90562661433093 31.0 30.0 34.0 26.0 34.0 2 30.11099984389262 31.0 30.0 34.0 26.0 34.0 3 30.182286380006257 31.0 30.0 34.0 26.0 34.0 4 33.78874445358669 35.0 35.0 37.0 30.0 37.0 5 33.291187295449916 35.0 35.0 37.0 27.0 37.0 6 33.217435080804314 35.0 35.0 37.0 27.0 37.0 7 32.98710443992559 35.0 33.0 37.0 26.0 37.0 8 32.8633017460167 35.0 33.0 37.0 26.0 37.0 9 34.21410365393517 38.0 34.0 39.0 27.0 39.0 10 33.97189930869654 37.0 34.0 39.0 25.0 39.0 11 33.96943840200581 37.0 34.0 39.0 25.0 39.0 12 33.73204202765078 37.0 33.0 39.0 25.0 39.0 13 33.69879933657773 37.0 33.0 39.0 25.0 39.0 14 34.531697637052396 38.0 33.0 40.0 24.0 41.0 15 34.43998693878886 38.0 33.0 40.0 23.0 41.0 16 34.30713001699457 38.0 33.0 40.0 22.0 41.0 17 34.22490096725222 38.0 33.0 40.0 21.0 41.0 18 34.1320388918694 38.0 33.0 40.0 19.0 41.0 19 34.1383520378148 38.0 33.0 40.0 19.0 41.0 20 33.99641157539612 38.0 33.0 40.0 18.0 41.0 21 33.84774827037798 38.0 33.0 40.0 18.0 41.0 22 33.69327149936023 38.0 33.0 40.0 18.0 41.0 23 33.73832177070758 38.0 33.0 40.0 17.0 41.0 24 33.563943013308666 38.0 33.0 40.0 10.0 41.0 25 33.074287342554804 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10948448145102674 1101 2 0.029304858222815255 1101 3 0.07904301036047556 1101 4 0.18430106795558743 1101 5 0.12467356220594183 1101 6 0.08962093931371129 1101 7 -0.00574518412103231 1101 8 0.0758038537889476 1101 9 0.09542609462703666 1101 10 0.11405544399946166 1101 11 0.20088165788521195 1101 12 -0.0288595522767352 1101 13 0.04002898893465101 1101 14 -0.09443257323082577 1101 15 8.602707913141217E-4 1101 16 -0.16925917118849299 1101 17 -0.022406211071235305 1101 18 -0.05872003509282564 1101 19 -0.03720549385138838 1101 20 -0.05072904244791232 1101 21 -0.3454597179154675 1101 22 -0.5603972752773814 1101 23 -0.2968297002074394 1101 24 -0.6754783144503236 1101 25 -0.5470470343882994 1102 1 0.24793187973491015 1102 2 0.21647180471526184 1102 3 0.4279802254193079 1102 4 0.47924960434509956 1102 5 0.5246065367429082 1102 6 0.35864190302041976 1102 7 0.5191239171405044 1102 8 0.41833383275223923 1102 9 0.42481311026852353 1102 10 0.474582755216602 1102 11 0.5034518521263323 1102 12 0.6805717930769433 1102 13 0.7582765053426215 1102 14 0.701063448376587 1102 15 0.2036985668845901 1102 16 0.386982492104778 1102 17 0.6018961600374695 1102 18 0.2763409182614325 1102 19 0.3874240411511636 1102 20 0.5778105324381144 1102 21 0.930608873687099 1102 22 0.9040128674669461 1102 23 0.8447267504956599 1102 24 0.7078376542003468 1102 25 0.6679487516345688 1103 1 0.31979249181399183 1103 2 0.36734426889457694 1103 3 0.5156941500249879 1103 4 0.4942871983300634 1103 5 0.6160508421891961 1103 6 0.4724749418782821 1103 7 0.4819684211965267 1103 8 0.6676116155454181 1103 9 0.7886783909659769 1103 10 0.6766584848494333 1103 11 0.41673097618003396 1103 12 0.6212726017022803 1103 13 0.5599698467579088 1103 14 0.7071990704506419 1103 15 0.5416317359994878 1103 16 0.7671041083237142 1103 17 0.8534083023142571 1103 18 0.9631049982727831 1103 19 0.6490923628989336 1103 20 0.8253537042613246 1103 21 0.9839046345300346 1103 22 0.9044707015891262 1103 23 0.5813334903298113 1103 24 0.9495772948972814 1103 25 0.8526009911527481 1104 1 0.27728422075773196 1104 2 0.29083497015394144 1104 3 0.20286026528790657 1104 4 0.3202785982345091 1104 5 0.12503677012508518 1104 6 0.2112204457591389 1104 7 0.3345263636642315 1104 8 0.4696701394661247 1104 9 0.43727479046475537 1104 10 0.2963751504643781 1104 11 0.5099433011807051 1104 12 0.3924685919302604 1104 13 0.564863968222987 1104 14 0.6559406078304093 1104 15 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-0.9175803756615935 2315 12 -0.8382303003520164 2315 13 -1.0123236721703606 2315 14 -1.065262134916786 2315 15 -0.9863117370298795 2315 16 -1.2777424137827396 2315 17 -1.1371138480220253 2315 18 -1.0802612384192756 2315 19 -1.091626366414559 2315 20 -1.1125721406625502 2315 21 -1.082862729336739 2315 22 -1.1624575827427037 2315 23 -1.0906493423823918 2315 24 -1.0046984350050323 2315 25 -0.9435630672613797 2316 1 -1.3186932023487614 2316 2 -1.4250829662987172 2316 3 -1.5111872145236482 2316 4 -1.4633327848301931 2316 5 -1.7712111464183842 2316 6 -1.8442331180740226 2316 7 -1.6668267605897213 2316 8 -1.773221139694055 2316 9 -1.9738375617768327 2316 10 -1.9634268965248225 2316 11 -1.9894037911791145 2316 12 -2.0623414822528368 2316 13 -2.0513524966139585 2316 14 -2.539222925711872 2316 15 -2.178858558201746 2316 16 -2.558274918254483 2316 17 -2.5913674060934326 2316 18 -2.3621314906587045 2316 19 -2.3719793789905417 2316 20 -2.326930781464611 2316 21 -2.5491111443433283 2316 22 -2.5862268414678304 2316 23 -2.4920380942828295 2316 24 -2.653048486853308 2316 25 -2.811218894216978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40025.0 3 2.0 4 705.0 5 5850.0 6 7098.0 7 5831.0 8 4633.0 9 4212.0 10 3924.0 11 3715.0 12 3467.0 13 3420.0 14 3286.0 15 3242.0 16 3407.0 17 3601.0 18 3811.0 19 4384.0 20 5172.0 21 6610.0 22 8468.0 23 10551.0 24 12656.0 25 15263.0 26 17416.0 27 19455.0 28 22819.0 29 27576.0 30 34676.0 31 43489.0 32 55255.0 33 70044.0 34 88963.0 35 117332.0 36 167747.0 37 271187.0 38 367647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.606946703245555 21.116710199924434 14.46632059095713 23.810022505872883 2 15.745780840239437 22.385866078957612 38.8296309526163 23.038722128186652 3 18.554691094858068 25.864333827105284 31.148807141946598 24.43216793609005 4 13.611404427859645 18.75640364050584 37.78371152447375 29.84848040716076 5 13.26247988874786 38.133593958337734 35.842053608635545 12.761872544278857 6 31.006742611655973 38.28864049561707 18.01874515572904 12.685871736997925 7 27.18252088191806 32.6040823783402 22.757728848984176 17.455667890757557 8 25.08608377616514 36.46611697576838 20.984058054994087 17.463741193072398 9 26.129375522772246 15.571472297075747 20.725878853858273 37.57327332629373 10 14.843289325595679 29.57137277010155 34.575875343146514 21.00946256115626 11 33.671611430332135 23.247456097356466 24.637084432277007 18.443848040034386 12 22.475508524894355 25.71586139573629 31.719928367846244 20.08870171152311 13 28.298381868639392 22.374209152527815 26.33374666567594 22.99366231315685 14 21.031072185005648 22.34639613508128 28.22121437905734 28.401317300855727 15 22.89733928950011 30.54571457981552 24.135086735031244 22.421859395653126 16 22.369300972978426 28.8132635372023 27.205630227296428 21.611805262522847 17 21.685223448282446 28.697989486952082 27.967079749055685 21.649707315709787 18 21.479216245528956 28.113507105612435 29.294605978844384 21.11267067001423 19 22.877994843681194 27.624480380220568 28.35477709351043 21.14274768258781 20 23.104096353018086 27.61185025416871 28.417541561032873 20.866511831780326 21 22.91935792831195 27.236170011159295 27.95317324033242 21.891298820196344 22 22.848121155685412 27.818130133563834 28.051200492999367 21.282548217751383 23 22.4224729180968 27.65663739255688 28.096737492152023 21.824152197194294 24 22.512252370541407 27.31128213413832 28.550528714757178 21.625936780563094 25 22.51370217871459 27.547650860305943 28.322948763395413 21.615698197584056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 818.0 1 818.0 2 744.5 3 671.0 4 671.0 5 671.0 6 1398.0 7 2125.0 8 2125.0 9 2125.0 10 3170.0 11 4215.0 12 4215.0 13 4215.0 14 6648.5 15 9082.0 16 9082.0 17 9082.0 18 15253.0 19 21424.0 20 21424.0 21 21424.0 22 32559.5 23 43695.0 24 43695.0 25 43695.0 26 60062.5 27 76430.0 28 76430.0 29 76430.0 30 94872.5 31 113315.0 32 113315.0 33 113315.0 34 128720.0 35 144125.0 36 144125.0 37 144125.0 38 155666.5 39 167208.0 40 167208.0 41 167208.0 42 174464.5 43 181721.0 44 181721.0 45 181721.0 46 183522.0 47 185323.0 48 185323.0 49 185323.0 50 179110.0 51 172897.0 52 172897.0 53 172897.0 54 155420.5 55 137944.0 56 137944.0 57 137944.0 58 117263.0 59 96582.0 60 96582.0 61 96582.0 62 77431.5 63 58281.0 64 58281.0 65 58281.0 66 44347.0 67 30413.0 68 30413.0 69 30413.0 70 21911.5 71 13410.0 72 13410.0 73 13410.0 74 9155.5 75 4901.0 76 4901.0 77 4901.0 78 3303.5 79 1706.0 80 1706.0 81 1706.0 82 1103.0 83 500.0 84 500.0 85 500.0 86 315.5 87 131.0 88 131.0 89 131.0 90 75.0 91 19.0 92 19.0 93 19.0 94 10.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4081287633637118 2 0.0 3 0.0 4 0.0 5 0.049081795493882155 6 0.0 7 0.0 8 0.003681134662041162 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.816916040816966E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005453532832653573 25 0.0012952140477552236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1466939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.90467384292484 #Duplication Level Percentage of deduplicated Percentage of total 1 85.45572434242617 66.57400332907602 2 10.433606287725212 16.25653389701445 3 2.420903357581802 5.657990594329557 4 0.7831397227191027 2.4404097868748114 5 0.3284773153898222 1.2794959060121827 6 0.17038157338694593 0.7964112542132631 7 0.09565957172764251 0.5216629414777104 8 0.06456703416010381 0.4024058989798299 9 0.041940022276314715 0.294059138076117 >10 0.1862912622641668 2.43143502015494 >50 0.009956197789226075 0.548557075506857 >100 0.007954797889896038 1.2444177545466748 >500 9.614788312462299E-4 0.5762136954707051 >1k 4.3703583238464993E-4 0.976403708266892 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4782 0.3259849250718673 No Hit TATCAACGCAGAGTACTTTTTTTTT 3392 0.23122979210451153 No Hit GGTATCAACGCAGAGTACTTTTTTT 3386 0.2308207771420625 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1594 0.10866164169062245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.816916040816966E-5 0.0 4 0.0 0.0 0.0 6.816916040816966E-5 0.0 5 0.0 0.0 0.0 6.816916040816966E-5 0.0 6 0.0 0.0 0.0 6.816916040816966E-5 0.0 7 0.0 0.0 0.0 6.816916040816966E-5 0.0 8 0.0 0.0 0.0 6.816916040816966E-5 0.0 9 0.0 0.0 0.0 6.816916040816966E-5 0.0 10 0.0 0.0 0.0 2.7267664163267865E-4 0.0 11 0.0 0.0 0.0 4.0901496244901797E-4 0.0 12 0.0 0.0 0.0 4.0901496244901797E-4 0.0 13 0.0 0.0 0.0 4.7718412285718766E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 45 6.754008E-4 14.780086 5 TACACCG 40 0.0052734497 14.252225 5 GGTATCA 870 0.0 13.478637 1 TGTACCG 50 0.0014977141 13.302077 5 TAGGACC 280 0.0 11.876854 4 ACCGTGC 65 8.039993E-4 11.68935 8 CGTGCGC 65 8.0445927E-4 11.688553 10 GTCCTAG 170 1.0913936E-11 11.216082 1 AATCCCG 80 3.7792264E-4 10.684796 19 GTCTAAT 75 0.0025708328 10.169248 1 TAAGTCC 85 6.5830146E-4 10.060394 4 TGGCGAG 690 0.0 10.048183 18 GTGCGCC 115 1.0299369E-5 9.90986 11 CTACACT 145 1.6122249E-7 9.829121 4 GTGTAAG 185 6.0754246E-10 9.791336 1 GTATCAA 1870 0.0 9.788581 1 GGCGAGG 340 0.0 9.7769375 19 GTAGGAC 700 0.0 9.772954 3 TTAGGAC 530 0.0 9.680757 3 GTGGTAT 355 0.0 9.667947 1 >>END_MODULE