FastQCFastQC Report
Thu 26 May 2016
SRR1512069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813141
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29630.36438944783254074No Hit
TATCAACGCAGAGTACTTTTTTTTT18390.22616003866488102No Hit
GGTATCAACGCAGAGTACTTTTTTT17740.2181663450742245No Hit
GTCCTACAGTGGACATTTCTAAATT14100.17340166096654822No Hit
CTGTAGGACGTGGAATATGGCAAGA12750.1567993742782617No Hit
GTCCTAAAGTGTGTATTTCTCATTT12690.15606149486989343No Hit
CTTTAGGACGTGAAATATGGCGAGG11330.13933622828021217No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8620.1060086750022444No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8160.10035159953808749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAG400.005419805614.19244318
GCCTCGA1001.0630174E-813.2462816
GGTATCA4500.012.900251
GAACAGT1251.4988473E-912.1108856
GTCCTAG655.9700216E-412.1097531
TAATGCA658.292994E-411.64508154
GTCTTAT1103.1469062E-711.6281141
TCGAACT1251.9383151E-811.35395419
GTAAGAC752.1508313E-411.3539533
GTCCTAT1052.270348E-611.2447691
CTAGGCA600.00605111411.0385674
TTGTACC700.001541236610.813294
GGCGAGG3500.010.8132919
GTATCAA9900.010.7336441
GTCCAAT650.0082001710.5960331
TAGAGTG1356.015034E-810.512925
ATTAGAG909.899243E-510.512923
GTATATA1603.450623E-910.4541231
GGACGTG8000.010.4077916
GACGTGA3750.010.3447137
Produced by FastQC (version 0.11.5)