FastQCFastQC Report
Thu 26 May 2016
SRR1512069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813141
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46750.574931039020293No Hit
TATCAACGCAGAGTACTTTTTTTTT37430.46031377092041853No Hit
GGTATCAACGCAGAGTACTTTTTTT36280.4461710822600262No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17030.20943477207519975No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13620.16749862569960192No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11790.14499330374436906No Hit
CTGTAGGACGTGGAATATGGCAAGA11010.1354008714355813No Hit
CTTTAGGACGTGAAATATGGCGAGG10100.12420970040866221No Hit
GTCCTAAAGTGTGTATTTCTCATTT8930.10982105194548054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.014.0457461
AGGACCG500.001495735113.3032295
TAGGACC2500.012.5430464
GACAGTC550.003073427412.08715257
AAATGTC2050.012.0450367
ACAGTCC1104.976391E-711.2237848
AATGTCC2050.011.1184958
ATCAGAT951.3625589E-510.99658215
AGGACCT5200.010.96425
CAAGAGT700.001487060910.8597794
GAACAGT1451.4448233E-810.4795026
GTCCTAA3550.010.4707221
GTAGAAC2001.8189894E-1210.4525383
TAGAAAT2550.010.4339074
ATGTCCA2400.010.2884699
GGACCTG5300.010.2138076
ACGAAAT750.002652077210.13018512
GAAATGT2450.010.0785016
TGTCCAC2450.010.07850110
GTATAAC856.57105E-410.0612673
Produced by FastQC (version 0.11.5)