##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512069_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 813141 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.869798718795387 31.0 28.0 31.0 16.0 34.0 2 28.061651054368184 31.0 28.0 31.0 16.0 34.0 3 28.02334281508373 31.0 27.0 31.0 16.0 34.0 4 31.467417090024977 35.0 32.0 37.0 20.0 37.0 5 30.82999627370899 35.0 30.0 37.0 16.0 37.0 6 30.827010321703124 35.0 31.0 37.0 11.0 37.0 7 30.527078329588594 35.0 30.0 37.0 10.0 37.0 8 30.34534232070453 35.0 30.0 37.0 10.0 37.0 9 31.364111513255388 37.0 30.0 39.0 10.0 39.0 10 30.995845738930885 35.0 30.0 39.0 8.0 39.0 11 31.065866805387994 35.0 30.0 39.0 2.0 39.0 12 30.73022759890351 35.0 30.0 39.0 2.0 39.0 13 30.701816782083306 35.0 30.0 39.0 2.0 39.0 14 31.128280335144826 36.0 29.0 40.0 2.0 41.0 15 31.033769051123976 36.0 29.0 40.0 2.0 41.0 16 30.84588404717017 36.0 29.0 39.0 2.0 41.0 17 30.749803539607523 36.0 29.0 39.0 2.0 41.0 18 30.60145042495705 36.0 28.0 39.0 2.0 41.0 19 30.62375897907005 36.0 28.0 39.0 2.0 41.0 20 30.428977262246033 36.0 28.0 39.0 2.0 41.0 21 30.218047054569873 36.0 27.0 39.0 2.0 40.0 22 30.003140906681622 36.0 27.0 39.0 2.0 40.0 23 29.967056144014382 36.0 27.0 39.0 2.0 40.0 24 29.690987171966487 36.0 27.0 39.0 2.0 41.0 25 29.1072126482369 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.320776338839309 1101 2 -0.4794104170854716 1101 3 -0.5036082610760211 1101 4 -0.7842836627245653 1101 5 -0.63592954335002 1101 6 -0.8190862097143743 1101 7 -0.6288007044027459 1101 8 -0.7619089121426832 1101 9 -0.8598193339392246 1101 10 -0.7257031021825675 1101 11 -0.9560412393398927 1101 12 -0.8151915805158545 1101 13 -0.7356926564202908 1101 14 -1.1195530425205185 1101 15 -1.1364692696842198 1101 16 -0.9202800798863748 1101 17 -0.8280341953951798 1101 18 -0.8864612941204015 1101 19 -1.3725935354340564 1101 20 -1.0312476249236653 1101 21 -1.3147451751206738 1101 22 -1.2764231609997658 1101 23 -1.0769417956845437 1101 24 -1.3445490789647359 1101 25 -1.3486052711799594 1102 1 -0.6908671686809917 1102 2 -0.650336431539511 1102 3 -0.573748302132234 1102 4 -0.5152459528738866 1102 5 -1.1057757231537373 1102 6 -0.8434612272898079 1102 7 -0.6435134515133463 1102 8 -0.9813501539525298 1102 9 -0.8719646616859151 1102 10 -0.8054055852398498 1102 11 -0.6780850513811743 1102 12 -0.8108356850123535 1102 13 -0.6124944898895386 1102 14 -0.6809109908346827 1102 15 -0.819882278317273 1102 16 -1.0952695346261194 1102 17 -0.7404185490410278 1102 18 -0.8191923759234996 1102 19 -0.9255054332996941 1102 20 -0.7794043134307387 1102 21 -0.9741107722636109 1102 22 -0.7149189850767037 1102 23 -0.773049891632354 1102 24 -0.7982877252345268 1102 25 -0.6279323429720911 1103 1 -0.36669231053346607 1103 2 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1107 11 0.7979118255358983 1107 12 1.1275168887425941 1107 13 0.7209475936529302 1107 14 0.8165208311495924 1107 15 0.7787931999048006 1107 16 0.8206749191132161 1107 17 0.7965732528662812 1107 18 0.7808215664110065 1107 19 0.9099248271431151 1107 20 0.9888852409722517 1107 21 0.5284936558218263 1107 22 0.7815034118894424 1107 23 0.5131471972082018 1107 24 0.45980719417877935 1107 25 0.4893589402803791 1108 1 -0.06712781800173673 1108 2 0.033413280641010346 1108 3 -0.1058401741101278 1108 4 0.036820590989801616 1108 5 -0.33281348506946173 1108 6 -0.3322017292469823 1108 7 -0.4222148100212877 1108 8 -0.329203020634548 1108 9 -0.18368467089613816 1108 10 -0.7346538007590766 1108 11 -0.37120040942860655 1108 12 -0.24750388671624535 1108 13 -0.49222048574493726 1108 14 -0.25551955634239576 1108 15 -0.040685870304379534 1108 16 -0.17225548903551058 1108 17 -0.25068794807395633 1108 18 0.20162848523238353 1108 19 -3.84549118251698E-4 1108 20 0.13211422214374835 1108 21 0.05849341838801081 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-1.0660941335609628 2315 11 -1.0446017143764195 2315 12 -0.9062915178474213 2315 13 -1.3751168267998821 2315 14 -1.3084828195158025 2315 15 -1.2077901991405113 2315 16 -1.3276199767698067 2315 17 -1.3985329084833928 2315 18 -1.484030151673256 2315 19 -1.4722625217599017 2315 20 -1.4889300710541775 2315 21 -1.440394562426306 2315 22 -1.6174916004903679 2315 23 -1.3222175286024864 2315 24 -1.3212194053805462 2315 25 -1.2233142229481722 2316 1 -0.985364596401439 2316 2 -1.247264738648628 2316 3 -1.2646610853086244 2316 4 -1.4797680504099127 2316 5 -1.4634940660641718 2316 6 -1.9260690073072588 2316 7 -2.034686471158942 2316 8 -1.8520005946998452 2316 9 -1.8214787866595898 2316 10 -1.9136609479162239 2316 11 -1.9721251849592 2316 12 -2.012348967602083 2316 13 -2.440044957713482 2316 14 -2.417468949858719 2316 15 -2.4201064126173435 2316 16 -2.148454046795557 2316 17 -2.2732053356471233 2316 18 -2.412196076395837 2316 19 -2.2061727217259417 2316 20 -2.2485279332748966 2316 21 -2.7063740774171947 2316 22 -2.725098292516762 2316 23 -2.6109000074601454 2316 24 -2.9309451457345403 2316 25 -2.7345886804070325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49744.0 3 3.0 4 788.0 5 5576.0 6 6628.0 7 5472.0 8 4460.0 9 3979.0 10 3624.0 11 3484.0 12 3151.0 13 3043.0 14 3131.0 15 3061.0 16 3080.0 17 3218.0 18 3501.0 19 4034.0 20 4810.0 21 6114.0 22 7654.0 23 9139.0 24 10909.0 25 12649.0 26 14252.0 27 16331.0 28 18420.0 29 22395.0 30 27067.0 31 33399.0 32 40958.0 33 50293.0 34 61702.0 35 76017.0 36 94433.0 37 110589.0 38 86033.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.66921922366719 24.090929288740114 16.091724185601013 19.148127301991686 2 18.378485404130405 25.29622783748452 37.54699369482046 18.77829306356462 3 21.26002747370997 28.615332396226485 30.91210503467418 19.21253509538936 4 16.95191854795171 21.5153337490054 36.48457032667151 25.048177376371378 5 13.461323135491762 40.396273752000994 34.88245043502013 11.259952677487108 6 30.343445970625023 40.31896067225733 17.528448325690132 11.809145031427514 7 27.02999848734721 34.121511521372064 22.910417750427047 15.938072240853677 8 25.646648202716467 38.254074449402054 20.388480856359976 15.710796491521506 9 27.433495543823273 15.963529080442385 20.880880437710065 35.72209493802428 10 16.22867374784939 31.19348304906529 33.32546261964407 19.252380583441248 11 34.16848984370484 23.758241190642213 24.83874260429618 17.234526361356764 12 23.668834802328256 26.970353235170773 31.29346570889919 18.067346253601773 13 29.855707681693584 23.37085450124886 25.888990962207046 20.88444685485051 14 21.725752360291757 23.986245927828016 28.19424921385098 26.09375249802925 15 23.657397671498547 32.46952250593685 23.71384544623872 20.159234376325877 16 22.28580283124329 29.795324550108777 27.695688693596804 20.22318392505113 17 21.79363726586164 30.200297365401575 27.876223188844246 20.12984217989254 18 21.667705846833453 28.413522378037758 30.422645027123217 19.496126748005572 19 23.84534028580564 27.832722532405246 28.921587918439627 19.400349263349483 20 23.943448922142654 28.27160357182826 29.01932137230812 18.765626133720968 21 23.543272323004253 27.484655182803476 28.58286570225828 20.389206791933994 22 23.79673389977876 28.527032827025078 28.67645340721966 18.999779865976503 23 22.723365320405687 28.571551551329964 28.971851130369764 19.733231997894585 24 23.299216820646024 27.97381858968683 29.25005165441719 19.476912935249956 25 23.13784617730722 28.281789773111587 29.09691058390346 19.483453465677734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 962.0 1 962.0 2 815.0 3 668.0 4 668.0 5 668.0 6 1366.5 7 2065.0 8 2065.0 9 2065.0 10 2799.5 11 3534.0 12 3534.0 13 3534.0 14 4911.5 15 6289.0 16 6289.0 17 6289.0 18 10577.0 19 14865.0 20 14865.0 21 14865.0 22 22589.5 23 30314.0 24 30314.0 25 30314.0 26 41027.0 27 51740.0 28 51740.0 29 51740.0 30 62738.5 31 73737.0 32 73737.0 33 73737.0 34 80727.5 35 87718.0 36 87718.0 37 87718.0 38 90406.0 39 93094.0 40 93094.0 41 93094.0 42 95581.5 43 98069.0 44 98069.0 45 98069.0 46 97092.5 47 96116.0 48 96116.0 49 96116.0 50 91765.0 51 87414.0 52 87414.0 53 87414.0 54 77195.0 55 66976.0 56 66976.0 57 66976.0 58 56047.0 59 45118.0 60 45118.0 61 45118.0 62 36260.5 63 27403.0 64 27403.0 65 27403.0 66 21404.0 67 15405.0 68 15405.0 69 15405.0 70 11311.0 71 7217.0 72 7217.0 73 7217.0 74 5062.0 75 2907.0 76 2907.0 77 2907.0 78 1974.5 79 1042.0 80 1042.0 81 1042.0 82 712.5 83 383.0 84 383.0 85 383.0 86 233.0 87 83.0 88 83.0 89 83.0 90 50.0 91 17.0 92 17.0 93 17.0 94 11.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3656192468464879 2 0.0 3 0.0 4 0.0 5 0.05165155858578033 6 0.0 7 0.0 8 0.0030744975348678764 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.2297990139471506E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005534095562762177 25 0.0012297990139471505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 813141.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.29425869328739 #Duplication Level Percentage of deduplicated Percentage of total 1 89.26382605581627 69.88845089166686 2 7.469199521888556 11.695908791970421 3 1.7298714635509658 4.063170116201849 4 0.6012393408142357 1.882943539451654 5 0.2968068707216997 1.1619136959114933 6 0.1657421696844336 0.7785996185795866 7 0.10420366687052698 0.5710984195525127 8 0.06970950441480864 0.43662831776271094 9 0.046515790584289514 0.3277727407196231 >10 0.21881614841249217 2.963047681894023 >50 0.017585242785167248 0.9844027427519028 >100 0.013501364877707868 2.1273107074705564 >500 0.0017269187035339599 0.8569717446048006 >1k 0.0012559408752974253 2.261780991461982 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4675 0.574931039020293 No Hit TATCAACGCAGAGTACTTTTTTTTT 3743 0.46031377092041853 No Hit GGTATCAACGCAGAGTACTTTTTTT 3628 0.4461710822600262 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1703 0.20943477207519975 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1362 0.16749862569960192 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1179 0.14499330374436906 No Hit CTGTAGGACGTGGAATATGGCAAGA 1101 0.1354008714355813 No Hit CTTTAGGACGTGAAATATGGCGAGG 1010 0.12420970040866221 No Hit GTCCTAAAGTGTGTATTTCTCATTT 893 0.10982105194548054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2297990139471506E-4 0.0 3 0.0 0.0 0.0 1.2297990139471506E-4 0.0 4 0.0 0.0 0.0 1.2297990139471506E-4 0.0 5 0.0 1.2297990139471506E-4 0.0 1.2297990139471506E-4 0.0 6 0.0 1.2297990139471506E-4 0.0 1.2297990139471506E-4 0.0 7 0.0 1.2297990139471506E-4 0.0 1.2297990139471506E-4 0.0 8 0.0 1.2297990139471506E-4 0.0 1.2297990139471506E-4 0.0 9 0.0 1.2297990139471506E-4 0.0 2.459598027894301E-4 0.0 10 0.0 1.2297990139471506E-4 0.0 2.459598027894301E-4 0.0 11 0.0 1.2297990139471506E-4 0.0 2.459598027894301E-4 0.0 12 0.0 1.2297990139471506E-4 0.0 2.459598027894301E-4 0.0 13 0.0 1.2297990139471506E-4 0.0 2.459598027894301E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 950 0.0 14.045746 1 AGGACCG 50 0.0014957351 13.303229 5 TAGGACC 250 0.0 12.543046 4 GACAGTC 55 0.0030734274 12.0871525 7 AAATGTC 205 0.0 12.045036 7 ACAGTCC 110 4.976391E-7 11.223784 8 AATGTCC 205 0.0 11.118495 8 ATCAGAT 95 1.3625589E-5 10.996582 15 AGGACCT 520 0.0 10.9642 5 CAAGAGT 70 0.0014870609 10.859779 4 GAACAGT 145 1.4448233E-8 10.479502 6 GTCCTAA 355 0.0 10.470722 1 GTAGAAC 200 1.8189894E-12 10.452538 3 TAGAAAT 255 0.0 10.433907 4 ATGTCCA 240 0.0 10.288469 9 GGACCTG 530 0.0 10.213807 6 ACGAAAT 75 0.0026520772 10.130185 12 GAAATGT 245 0.0 10.078501 6 TGTCCAC 245 0.0 10.078501 10 GTATAAC 85 6.57105E-4 10.061267 3 >>END_MODULE