FastQCFastQC Report
Thu 26 May 2016
SRR1512068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914758
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30740.33604516167117426No Hit
GGTATCAACGCAGAGTACTTTTTTT18900.2066120219773973No Hit
TATCAACGCAGAGTACTTTTTTTTT18280.19983427310829752No Hit
GTCCTACAGTGGACATTTCTAAATT10660.11653355313645795No Hit
GTCCTAAAGTGTGTATTTCTCATTT10370.11336331576220159No Hit
CTGTAGGACGTGGAATATGGCAAGA9510.10396192216957927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC350.002226656516.22218510
GGTATCA4300.012.3410441
GTGCGCC701.1304497E-412.16663811
TAGGACC2500.011.7340464
GTATCAA10500.011.6989611
TTAGGAC4750.011.5547493
GCCCTAA600.00456282511.4649821
AGGACGT6500.011.355535
GACGTGA3450.011.2458137
GGTCTAG700.001123209711.2310031
ATTACTC855.53299E-511.1328723
GGACGTG6300.010.9649946
GGCGTGC700.001539773410.814798
TAGGGTG700.001539773410.814795
ACGTGAA3800.010.7080648
GGCGAGG3550.010.66246819
TGTAGGA7100.010.6575892
GCTCTAA750.00199706610.4822691
GTAGGAC7100.010.2626263
TAGGACA3050.010.2385924
Produced by FastQC (version 0.11.5)