FastQCFastQC Report
Thu 26 May 2016
SRR1512068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914758
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46470.5080032095920451No Hit
GGTATCAACGCAGAGTACTTTTTTT36370.39759149414380635No Hit
TATCAACGCAGAGTACTTTTTTTTT36200.3957330791313112No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16120.17622147059659496No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13280.14517500803491196No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11930.13041700646509788No Hit
CTGTAGGACGTGGAATATGGCAAGA9340.10210350715708418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.012.2985661
TAGGACC2550.011.1769534
AGTACTG855.3147363E-511.1769535
GCTAGAC951.3577384E-511.0004753
ATAGGAC1408.3200575E-910.8576113
CTAATAC700.001489849510.8576113
TGTAGGA6750.010.6967572
ACTTACC803.775499E-410.685048
TTCCACC2800.010.51486111
TAATACC1002.3942706E-510.4504514
TAGGACT1659.240466E-1010.3640844
GGACGTG5950.010.2161356
GTAGTAC750.002644843410.1337713
CTGTAGG6800.010.0945831
CCACCTT2850.09.99715113
GGCGAGG2201.8189894E-129.92953219
GTAGGAC6700.09.9258013
AGGACGT6150.09.8866055
CTAGGAC1451.6116064E-79.828013
TAGGACG6250.09.728424
Produced by FastQC (version 0.11.5)