##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512068_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 914758 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.144397753285567 31.0 28.0 31.0 20.0 34.0 2 28.334149578358428 31.0 28.0 31.0 22.0 34.0 3 28.31365016758531 31.0 28.0 31.0 20.0 34.0 4 31.790945802059124 35.0 32.0 37.0 23.0 37.0 5 31.182181516860197 35.0 32.0 37.0 17.0 37.0 6 31.169427323947975 35.0 32.0 37.0 17.0 37.0 7 30.872878947218826 35.0 31.0 37.0 13.0 37.0 8 30.698132183593913 35.0 31.0 37.0 11.0 37.0 9 31.757995010702285 37.0 31.0 39.0 10.0 39.0 10 31.394005846354993 35.0 30.0 39.0 10.0 39.0 11 31.474156006288002 37.0 30.0 39.0 10.0 39.0 12 31.13316855386889 35.0 30.0 39.0 8.0 39.0 13 31.10978204071459 35.0 30.0 39.0 8.0 39.0 14 31.58592983062187 37.0 30.0 40.0 2.0 41.0 15 31.48107477606099 37.0 30.0 40.0 2.0 41.0 16 31.299868380489702 36.0 30.0 40.0 2.0 41.0 17 31.21215556464114 36.0 30.0 40.0 2.0 41.0 18 31.066725844430987 36.0 30.0 39.0 2.0 41.0 19 31.074828533885466 37.0 30.0 40.0 2.0 41.0 20 30.88629670360904 36.0 29.0 40.0 2.0 41.0 21 30.685980335782798 36.0 29.0 39.0 2.0 41.0 22 30.46711589294655 36.0 29.0 39.0 2.0 40.0 23 30.44833168991143 36.0 29.0 39.0 2.0 41.0 24 30.174627606427055 36.0 29.0 39.0 2.0 41.0 25 29.599943373001384 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06177656443204427 1101 2 -0.22809005454789144 1101 3 -0.29587652869884096 1101 4 -0.3061518062077475 1101 5 -0.0321761659240245 1101 6 -0.159446660323038 1101 7 -0.2705943672543789 1101 8 -0.3372199599943819 1101 9 -0.2100718029373354 1101 10 -0.03818464806558097 1101 11 -0.34396086032237605 1101 12 -0.4749032474062247 1101 13 -0.30544958415080004 1101 14 0.025183682685316455 1101 15 0.05636842695018984 1101 16 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0.48747975467228954 1103 3 0.3664592167931211 1103 4 0.5349240502772439 1103 5 0.6850309170508595 1103 6 0.5512788006016791 1103 7 0.3194377667469368 1103 8 0.6391012450728404 1103 9 0.5925349730355371 1103 10 0.5347135689612195 1103 11 0.48406213952580757 1103 12 0.4759669430362301 1103 13 0.2643585817652294 1103 14 0.6099634677839134 1103 15 0.5576641188011386 1103 16 0.8057197473178945 1103 17 0.8027127917206762 1103 18 0.9887925200863883 1103 19 0.49588169347082456 1103 20 0.7247512744070228 1103 21 0.9366964142321379 1103 22 0.7760557495165763 1103 23 0.48480681753867216 1103 24 0.8641751776784758 1103 25 0.6932690447676606 1104 1 0.49687966953466045 1104 2 0.619952816889878 1104 3 0.6954767118690093 1104 4 0.694291446870924 1104 5 0.3470124371528165 1104 6 0.6366469502946472 1104 7 0.8147019308067236 1104 8 0.6804348101290323 1104 9 0.9710325399455151 1104 10 0.6620887282706853 1104 11 0.8079020769606338 1104 12 0.9348144114639894 1104 13 1.2630623820201237 1104 14 1.1227082250542182 1104 15 1.1703321178742456 1104 16 1.2346966908933794 1104 17 1.2943490447620398 1104 18 1.4840856495036086 1104 19 1.4202893519113289 1104 20 1.0443043389418385 1104 21 1.0333755360024988 1104 22 1.2690750983753425 1104 23 1.1836829616702893 1104 24 1.1630759423639105 1104 25 1.2196202786918846 1105 1 0.34523725065828614 1105 2 0.5072978306805886 1105 3 0.28541996439361483 1105 4 0.2198843255669587 1105 5 0.3535836640440948 1105 6 0.4717966978066137 1105 7 0.38084027674137033 1105 8 0.49699895605237643 1105 9 0.07685699621250563 1105 10 -0.1423291751644129 1105 11 -0.10379082844140086 1105 12 -0.14440377875585142 1105 13 -0.019583484257069017 1105 14 0.18140047333356435 1105 15 0.1126914875240459 1105 16 0.025200880189039765 1105 17 0.21378447331573724 1105 18 0.04498696031377136 1105 19 0.4988304730535056 1105 20 0.061024953201954446 1105 21 0.21529546455010262 1105 22 -0.11416151344245051 1105 23 -0.29376376762064993 1105 24 -0.11944470491856052 1105 25 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-0.49962709037943753 2315 11 -0.2896710717074029 2315 12 -0.08622780665852048 2315 13 -0.27158464167799323 2315 14 -0.29296693514649164 2315 15 -0.42928643064940886 2315 16 -0.6564292148310642 2315 17 -0.8095430080351242 2315 18 -0.7286983224044548 2315 19 -0.7314534713643397 2315 20 -0.6045374923817448 2315 21 -0.33902092398520267 2315 22 -0.41777819431736773 2315 23 -0.555486222754368 2315 24 -0.3286667026634049 2315 25 -0.6477718587732433 2316 1 -1.1880318461490411 2316 2 -1.226606597571955 2316 3 -1.178805460050505 2316 4 -1.2592773623452196 2316 5 -1.6148048466006948 2316 6 -1.8669590288203608 2316 7 -2.110700077864589 2316 8 -1.8819931576005118 2316 9 -2.2465086587712477 2316 10 -2.279946186488008 2316 11 -1.9682191318344096 2316 12 -1.6150892960463246 2316 13 -1.6431896448619447 2316 14 -1.9312380207859974 2316 15 -1.8922343014920173 2316 16 -2.258436856312393 2316 17 -2.021293246101152 2316 18 -1.8372593520181155 2316 19 -2.146458057421558 2316 20 -2.2622949396128753 2316 21 -2.561789383595766 2316 22 -2.164268546070879 2316 23 -1.802635272534996 2316 24 -1.8066068911299098 2316 25 -2.0527864348404528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50275.0 3 3.0 4 803.0 5 5804.0 6 6914.0 7 5653.0 8 4713.0 9 4220.0 10 3860.0 11 3685.0 12 3427.0 13 3340.0 14 3259.0 15 3265.0 16 3334.0 17 3495.0 18 3740.0 19 4287.0 20 5111.0 21 6542.0 22 8150.0 23 10135.0 24 11682.0 25 13596.0 26 15534.0 27 17666.0 28 20649.0 29 24338.0 30 29951.0 31 36809.0 32 45768.0 33 56611.0 34 70085.0 35 86922.0 36 108367.0 37 129433.0 38 103332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.20192356221076 24.074480434129075 16.03999850784588 19.68359749581429 2 17.945183316243206 25.397427516348586 37.479311468169726 19.17807769923849 3 20.744721554771864 28.41735191165314 31.13402670433054 19.703899829244456 4 16.47976841962574 21.50732762107574 36.70402445236882 25.3088795069297 5 13.417533789917382 40.089858540608056 35.182309154502875 11.310298514971684 6 30.16174769720516 40.35384221837907 17.700419127244583 11.783990957171186 7 26.693835965359142 33.98997330441494 23.153774003616256 16.16241672660966 8 25.22416450755961 38.11310441332415 20.833797951308036 15.828933127808206 9 26.984076662898822 15.983134337168956 20.963358615065406 36.06943038486682 10 15.549795683667156 31.098170226442402 34.02462727847146 19.327406811418975 11 34.04987985893537 23.836686861443134 24.798799245264867 17.314634034356626 12 22.936886039805064 26.93225967960925 31.730577923341475 18.40027635724421 13 29.282280122174388 23.276210757380642 26.365661737858538 21.07584738258643 14 21.482293677672125 23.63411962508117 28.453208389541278 26.430378307705425 15 23.368475596824513 32.00070401133415 24.10495453442331 20.525865857418026 16 22.272229376512694 29.729392910474683 27.737499972670363 20.260877740342252 17 21.71678192483695 30.002361280251172 28.024570432835787 20.25628636207609 18 21.542309550722706 28.756567310698568 30.019087015363628 19.682036123215102 19 23.265960249552613 28.234055601651587 28.928119708294115 19.571864440501685 20 23.455493146821347 28.24517522667197 29.087474501452842 19.21185712505384 21 23.170718375789008 27.653215385927204 28.64298535787607 20.533080880407713 22 23.30397766403792 28.504369461649965 28.741699990598608 19.449952883713507 23 22.65768651381021 28.379746337282647 28.928743995679735 20.033823153227413 24 22.8370490788894 28.070068032416795 29.20211957656571 19.890763312128094 25 22.8699801584048 28.29815959639025 29.04754877042236 19.78431147478259 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 891.0 1 891.0 2 777.0 3 663.0 4 663.0 5 663.0 6 1429.0 7 2195.0 8 2195.0 9 2195.0 10 3067.5 11 3940.0 12 3940.0 13 3940.0 14 5661.5 15 7383.0 16 7383.0 17 7383.0 18 12380.5 19 17378.0 20 17378.0 21 17378.0 22 25755.0 23 34132.0 24 34132.0 25 34132.0 26 46078.0 27 58024.0 28 58024.0 29 58024.0 30 70243.5 31 82463.0 32 82463.0 33 82463.0 34 90633.0 35 98803.0 36 98803.0 37 98803.0 38 102574.0 39 106345.0 40 106345.0 41 106345.0 42 109209.5 43 112074.0 44 112074.0 45 112074.0 46 110504.0 47 108934.0 48 108934.0 49 108934.0 50 103597.0 51 98260.0 52 98260.0 53 98260.0 54 86242.0 55 74224.0 56 74224.0 57 74224.0 58 62362.5 59 50501.0 60 50501.0 61 50501.0 62 40529.5 63 30558.0 64 30558.0 65 30558.0 66 23331.5 67 16105.0 68 16105.0 69 16105.0 70 11746.0 71 7387.0 72 7387.0 73 7387.0 74 5157.0 75 2927.0 76 2927.0 77 2927.0 78 2020.0 79 1113.0 80 1113.0 81 1113.0 82 718.5 83 324.0 84 324.0 85 324.0 86 214.5 87 105.0 88 105.0 89 105.0 90 63.0 91 21.0 92 21.0 93 21.0 94 13.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.363374794207867 2 0.0 3 0.0 4 0.0 5 0.047225605023405094 6 0.0 7 0.0 8 0.0030609188441095897 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0931853014677106E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00513797091689824 25 0.0014211408919080238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 914758.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.77944948810911 #Duplication Level Percentage of deduplicated Percentage of total 1 89.91768446677989 72.6350105047198 2 7.28746036563293 11.773540730044852 3 1.5353767079624057 3.7208065567820525 4 0.5171519412793075 1.6710099647299759 5 0.23031300897488954 0.9302279037470755 6 0.12469833408635209 0.60438376677479 7 0.08927343466466803 0.5048021234287192 8 0.052540607816151295 0.3395361100127476 9 0.03869310798393268 0.2813047165733437 >10 0.18004373670729934 2.514825701180071 >50 0.013925177241065956 0.796748377279548 >100 0.010676734812832387 1.7674984571168406 >500 0.0013514850364407232 0.7095102398109064 >1k 8.108910218644339E-4 1.7507948477992779 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4647 0.5080032095920451 No Hit GGTATCAACGCAGAGTACTTTTTTT 3637 0.39759149414380635 No Hit TATCAACGCAGAGTACTTTTTTTTT 3620 0.3957330791313112 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1612 0.17622147059659496 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1328 0.14517500803491196 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1193 0.13041700646509788 No Hit CTGTAGGACGTGGAATATGGCAAGA 934 0.10210350715708418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0931853014677106E-4 0.0 4 0.0 0.0 0.0 1.0931853014677106E-4 0.0 5 0.0 0.0 0.0 1.0931853014677106E-4 0.0 6 0.0 0.0 0.0 1.0931853014677106E-4 0.0 7 0.0 0.0 0.0 1.0931853014677106E-4 0.0 8 0.0 0.0 0.0 1.0931853014677106E-4 0.0 9 0.0 0.0 0.0 2.1863706029354212E-4 0.0 10 0.0 0.0 0.0 3.279555904403132E-4 0.0 11 0.0 0.0 0.0 4.3727412058708424E-4 0.0 12 0.0 0.0 0.0 4.3727412058708424E-4 0.0 13 0.0 0.0 0.0 4.3727412058708424E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1000 0.0 12.298566 1 TAGGACC 255 0.0 11.176953 4 AGTACTG 85 5.3147363E-5 11.176953 5 GCTAGAC 95 1.3577384E-5 11.000475 3 ATAGGAC 140 8.3200575E-9 10.857611 3 CTAATAC 70 0.0014898495 10.857611 3 TGTAGGA 675 0.0 10.696757 2 ACTTACC 80 3.775499E-4 10.68504 8 TTCCACC 280 0.0 10.514861 11 TAATACC 100 2.3942706E-5 10.450451 4 TAGGACT 165 9.240466E-10 10.364084 4 GGACGTG 595 0.0 10.216135 6 GTAGTAC 75 0.0026448434 10.133771 3 CTGTAGG 680 0.0 10.094583 1 CCACCTT 285 0.0 9.997151 13 GGCGAGG 220 1.8189894E-12 9.929532 19 GTAGGAC 670 0.0 9.925801 3 AGGACGT 615 0.0 9.886605 5 CTAGGAC 145 1.6116064E-7 9.82801 3 TAGGACG 625 0.0 9.72842 4 >>END_MODULE