FastQCFastQC Report
Thu 26 May 2016
SRR1512067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106810
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28860.2607493607755622No Hit
GGTATCAACGCAGAGTACTTTTTTT18500.1671470261381809No Hit
TATCAACGCAGAGTACTTTTTTTTT17760.16046114509265366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA350.002227242316.2219811
GTACCGA653.5099347E-614.5581856
ACCGATC400.00541691214.1942318
TAAGGGG400.00541691214.1942314
TCCAACG905.663169E-712.61709418
GGTATCA4850.011.7533441
GCATAAG600.004560088311.4662651
CTATTCC752.1496518E-411.3553864
TTAGACT855.536137E-511.13273054
CGATCTT600.00604778211.03995710
GTAGAAC1900.010.9569513
CGGTTTC700.001540378810.81465213
TGCGTTG700.001540378810.81465212
GTATCAA10950.010.770661
GTCTTAA1209.834548E-710.6472461
CTACACT1701.4915713E-1010.5760944
GGATAAG750.001995798410.4834421
TCTGCGT750.002734027610.09367610
ATAGTAG750.002734027610.0936763
GTCCTAA3450.09.9706651
Produced by FastQC (version 0.11.5)