##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512067_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1106810 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.81643732890017 31.0 30.0 34.0 25.0 34.0 2 27.41766427842177 31.0 27.0 34.0 10.0 34.0 3 29.044920085651558 31.0 28.0 34.0 16.0 34.0 4 33.33682565209928 35.0 33.0 37.0 28.0 37.0 5 33.70670937197893 37.0 35.0 37.0 28.0 37.0 6 33.9599940369169 37.0 35.0 37.0 30.0 37.0 7 34.018824369132915 37.0 35.0 37.0 30.0 37.0 8 34.06031026102041 37.0 35.0 37.0 30.0 37.0 9 35.7087341097388 39.0 37.0 39.0 30.0 39.0 10 35.60352092951816 39.0 37.0 39.0 30.0 39.0 11 35.61889935942032 39.0 37.0 39.0 30.0 39.0 12 35.53465635474923 39.0 37.0 39.0 30.0 39.0 13 35.52883512075243 39.0 37.0 39.0 30.0 39.0 14 36.73614080104083 40.0 37.0 41.0 31.0 41.0 15 36.6735681824342 40.0 37.0 41.0 30.0 41.0 16 36.63565381592143 40.0 37.0 41.0 30.0 41.0 17 36.585108555217246 40.0 37.0 41.0 30.0 41.0 18 36.54383859921757 40.0 37.0 41.0 30.0 41.0 19 36.562001608225444 40.0 37.0 41.0 30.0 41.0 20 36.50268248389516 40.0 37.0 41.0 30.0 41.0 21 36.445772083736145 40.0 37.0 41.0 30.0 41.0 22 36.355538891047246 40.0 37.0 41.0 30.0 41.0 23 36.266750390762645 40.0 37.0 41.0 30.0 41.0 24 36.178033266775685 40.0 37.0 41.0 30.0 41.0 25 36.0600780621787 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8848226716397818 1101 2 3.2678166118432657 1101 3 1.682843611604799 1101 4 1.0753612535348367 1101 5 0.6107787650069127 1101 6 0.19675689764181925 1101 7 0.07020639597242706 1101 8 0.15471392488881008 1101 9 0.09239617976015779 1101 10 -0.16331134813757586 1101 11 0.19259266168283773 1101 12 -0.11484546346268587 1101 13 -0.14615806540710707 1101 14 0.0023406852205027917 1101 15 0.13581764279234676 1101 16 -0.008649797864386244 1101 17 -0.07817848930545779 1101 18 -0.11042981088105819 1101 19 -0.097940057909355 1101 20 -0.15219610100584902 1101 21 0.029073435720931684 1101 22 0.02844119487495078 1101 23 -0.00880742564568493 1101 24 -0.005514621644678641 1101 25 -0.13290364187076875 1102 1 1.5426622159704095 1102 2 4.186860236004339 1102 3 2.5701294646602584 1102 4 1.723177837780682 1102 5 1.3440499147773153 1102 6 1.1448317828414005 1102 7 0.9704956047325837 1102 8 1.0330641103356513 1102 9 1.1263450935715298 1102 10 0.9126280284078092 1102 11 1.094664588563795 1102 12 1.0302887647252348 1102 13 1.0637666520072386 1102 14 1.2720388925677355 1102 15 1.220753398708986 1102 16 1.3014272508816447 1102 17 0.9784435082219218 1102 18 1.1128625328137502 1102 19 0.9973056591517562 1102 20 1.3432449975459022 1102 21 1.2368346066852496 1102 22 1.2434325848431556 1102 23 1.1386504072755415 1102 24 1.1921187234630537 1102 25 1.2344356375358032 1103 1 0.7781159420616817 1103 2 2.8461608922799755 1103 3 1.6755073088811443 1103 4 0.8938297418435468 1103 5 0.5753931480142214 1103 6 0.42027325773453583 1103 7 0.2142553193390384 1103 8 0.2700404660629516 1103 9 0.259801523561606 1103 10 0.2421522015868618 1103 11 0.2653239135065917 1103 12 0.2833650567678916 1103 13 0.3011774665230149 1103 14 0.13355245523774073 1103 15 0.1153640858554823 1103 16 0.03682633231163379 1103 17 0.17414627904356195 1103 18 0.1024200055049107 1103 19 0.21926350315676046 1103 20 0.3544802097560833 1103 21 0.38958802298851936 1103 22 0.37010093357594087 1103 23 0.3625808560903607 1103 24 0.32671459583471574 1103 25 0.24621459785703337 1104 1 1.289678329230437 1104 2 3.8187243978540515 1104 3 2.3922384763513342 1104 4 1.7107574262026048 1104 5 1.1838415452262723 1104 6 0.8908224821329824 1104 7 0.7894563918159037 1104 8 0.7607352118611388 1104 9 0.938781938936387 1104 10 0.8533244678445513 1104 11 0.8083533896850952 1104 12 0.8316925632388603 1104 13 0.6489527476654544 1104 14 0.7944927421483357 1104 15 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-1.261919216543724 2315 19 -1.130171922142246 2315 20 -1.42909339221832 2315 21 -1.3822586579253198 2315 22 -1.5725576794321796 2315 23 -1.5004555682524483 2315 24 -1.4525482784365948 2315 25 -1.6195075404729167 2316 1 -1.2493282354652422 2316 2 -1.2792989246916697 2316 3 -0.8484602702963784 2316 4 -1.1969505762687689 2316 5 -0.9554277087210394 2316 6 -1.2647194750371753 2316 7 -1.3748469449630463 2316 8 -1.3875870169067142 2316 9 -1.514845547757993 2316 10 -1.6673454066511795 2316 11 -1.8688490204972368 2316 12 -1.7747265883965682 2316 13 -2.007712062370828 2316 14 -2.390950824880953 2316 15 -2.548189693732972 2316 16 -2.036308601351294 2316 17 -1.9421436476796003 2316 18 -2.004047617754587 2316 19 -2.012731949262836 2316 20 -2.034877813960861 2316 21 -2.3006382896677096 2316 22 -2.212276520635484 2316 23 -2.116252659308522 2316 24 -1.9635605848384046 2316 25 -1.9711918203104517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36342.0 3 2.0 4 294.0 5 1436.0 6 1642.0 7 1493.0 8 1383.0 9 1295.0 10 1243.0 11 1160.0 12 1121.0 13 1211.0 14 1183.0 15 1154.0 16 1206.0 17 1398.0 18 1580.0 19 1977.0 20 2481.0 21 3048.0 22 3793.0 23 4342.0 24 5102.0 25 5845.0 26 6670.0 27 7723.0 28 9069.0 29 11249.0 30 14438.0 31 19137.0 32 26311.0 33 36815.0 34 53338.0 35 79541.0 36 126774.0 37 191795.0 38 442219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38333589324274 18.51103622121231 12.940251714386388 26.165376171158556 2 16.041620527989128 21.25357066443449 39.059878306500956 23.64493050107542 3 17.694906984938697 24.21924268844698 30.213406094993722 27.8724442316206 4 12.33960661721524 16.857455209114484 37.724089952204984 33.07884822146529 5 13.574958664992185 36.609535511966826 35.55813554268574 14.257370280355255 6 31.041009748737363 36.46931270949847 18.49522501603708 13.99445252572709 7 27.16708378131748 31.370153865613794 22.947027945175773 18.515734407892953 8 24.715938865417904 35.52625397089277 21.31678237519922 18.441024788490104 9 25.56446002475583 15.62490400339715 20.449670675183636 38.36096529666339 10 15.312926337853833 27.82817285712995 33.910879012658 22.94802179235822 11 33.18744861358318 22.577136093819174 24.807148471734084 19.428266820863563 12 22.73452534762064 24.239932779790568 31.5450709697238 21.48047090286499 13 28.020346762316926 21.488602379812253 26.272350267886992 24.218700589983825 14 21.228219966480093 21.09956135001197 27.763698212422245 29.90852047108569 15 22.973771469357885 28.94878073020663 24.11317208915713 23.964275711278358 16 22.582557078450684 27.180907292127827 27.03535385477182 23.20118177464967 17 21.77464966886819 27.424851600545715 27.841544619221004 22.95895411136509 18 21.64002855051906 26.872272567107274 28.95591835997145 22.53178052240222 19 23.02029443280936 26.46886626924682 27.771262533813513 22.739576764130298 20 23.053772650721715 26.52266698469834 28.158994152554012 22.264566212025937 21 23.053772650721715 25.970358002471972 27.55834797001829 23.417521376788024 22 22.900983371943003 26.474244762396953 27.67445726614649 22.950314599513554 23 22.508883694749652 26.404247187620562 27.829162009860838 23.25770710776895 24 22.53870819393083 26.164904992469328 28.13354089737416 23.162845916225685 25 22.725761422466366 26.17676023888472 27.89394747065892 23.20353086798999 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 550.0 1 550.0 2 521.5 3 493.0 4 493.0 5 493.0 6 1030.0 7 1567.0 8 1567.0 9 1567.0 10 2142.0 11 2717.0 12 2717.0 13 2717.0 14 4111.0 15 5505.0 16 5505.0 17 5505.0 18 9411.0 19 13317.0 20 13317.0 21 13317.0 22 19908.0 23 26499.0 24 26499.0 25 26499.0 26 37312.5 27 48126.0 28 48126.0 29 48126.0 30 61069.5 31 74013.0 32 74013.0 33 74013.0 34 84816.0 35 95619.0 36 95619.0 37 95619.0 38 105662.5 39 115706.0 40 115706.0 41 115706.0 42 124605.5 43 133505.0 44 133505.0 45 133505.0 46 137888.0 47 142271.0 48 142271.0 49 142271.0 50 140846.5 51 139422.0 52 139422.0 53 139422.0 54 127614.5 55 115807.0 56 115807.0 57 115807.0 58 99946.0 59 84085.0 60 84085.0 61 84085.0 62 69359.0 63 54633.0 64 54633.0 65 54633.0 66 42626.5 67 30620.0 68 30620.0 69 30620.0 70 22285.5 71 13951.0 72 13951.0 73 13951.0 74 9796.5 75 5642.0 76 5642.0 77 5642.0 78 3788.0 79 1934.0 80 1934.0 81 1934.0 82 1262.5 83 591.0 84 591.0 85 591.0 86 380.5 87 170.0 88 170.0 89 170.0 90 106.0 91 42.0 92 42.0 93 42.0 94 32.0 95 22.0 96 22.0 97 22.0 98 12.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.720783151579765 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.420984631508569E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.517487192923808E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 3.613989754339046E-4 20 1.806994877169523E-4 21 1.806994877169523E-4 22 5.420984631508569E-4 23 6.32448207009333E-4 24 2.7104923157542847E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1106810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.06108720945622 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1909981388515 66.41972024248113 2 9.483579038908594 14.616298227502122 3 2.25043631951438 5.20263208432276 4 0.8089893372319553 2.4936639147180757 5 0.416804531202875 1.605970516416063 6 0.23195228379971092 1.0724697102193237 7 0.13619078954988278 0.7346507217439749 8 0.10515350639918034 0.6482594821605879 9 0.06533101368673139 0.45310310488758454 >10 0.2889017069935669 3.7397549282329674 >50 0.011964584857831093 0.6300159473747491 >100 0.008279423004699605 1.2025778130205111 >500 0.0010644944993007301 0.5873427006633359 >1k 3.5483149976691004E-4 0.593540606256928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2886 0.2607493607755622 No Hit GGTATCAACGCAGAGTACTTTTTTT 1850 0.1671470261381809 No Hit TATCAACGCAGAGTACTTTTTTTTT 1776 0.16046114509265366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.034974385847616E-5 2 0.0 0.0 0.0 0.0 9.034974385847616E-5 3 0.0 0.0 0.0 0.0 9.034974385847616E-5 4 0.0 0.0 0.0 0.0 9.034974385847616E-5 5 0.0 9.034974385847616E-5 0.0 0.0 9.034974385847616E-5 6 0.0 9.034974385847616E-5 0.0 0.0 9.034974385847616E-5 7 0.0 9.034974385847616E-5 0.0 9.034974385847616E-5 9.034974385847616E-5 8 0.0 9.034974385847616E-5 0.0 9.034974385847616E-5 9.034974385847616E-5 9 0.0 9.034974385847616E-5 0.0 1.806994877169523E-4 2.7104923157542847E-4 10 0.0 2.7104923157542847E-4 0.0 1.806994877169523E-4 2.7104923157542847E-4 11 0.0 2.7104923157542847E-4 0.0 1.806994877169523E-4 2.7104923157542847E-4 12 0.0 2.7104923157542847E-4 0.0 1.806994877169523E-4 2.7104923157542847E-4 13 0.0 2.7104923157542847E-4 9.034974385847616E-5 1.806994877169523E-4 2.7104923157542847E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGCGA 35 0.0022272423 16.22198 11 GTACCGA 65 3.5099347E-6 14.558185 6 ACCGATC 40 0.005416912 14.194231 8 TAAGGGG 40 0.005416912 14.194231 4 TCCAACG 90 5.663169E-7 12.617094 18 GGTATCA 485 0.0 11.753344 1 GCATAAG 60 0.0045600883 11.466265 1 CTATTCC 75 2.1496518E-4 11.355386 4 TTAGACT 85 5.536137E-5 11.1327305 4 CGATCTT 60 0.006047782 11.039957 10 GTAGAAC 190 0.0 10.956951 3 CGGTTTC 70 0.0015403788 10.814652 13 TGCGTTG 70 0.0015403788 10.814652 12 GTATCAA 1095 0.0 10.77066 1 GTCTTAA 120 9.834548E-7 10.647246 1 CTACACT 170 1.4915713E-10 10.576094 4 GGATAAG 75 0.0019957984 10.483442 1 TCTGCGT 75 0.0027340276 10.093676 10 ATAGTAG 75 0.0027340276 10.093676 3 GTCCTAA 345 0.0 9.970665 1 >>END_MODULE