FastQCFastQC Report
Thu 26 May 2016
SRR1512067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106810
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39690.3585981333742919No Hit
TATCAACGCAGAGTACTTTTTTTTT30760.27791581210867267No Hit
GGTATCAACGCAGAGTACTTTTTTT29940.27050713311227764No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14130.1276641880720268No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12120.10950388955647311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC655.4653057E-513.1498439
GGTATCA8350.013.0084251
TAGACCT701.08747554E-412.2177434
GGCTAAT657.823898E-411.7268931
TAGACCA1552.5465852E-1111.6484584
GTAGACC1658.0035534E-1110.9424913
GCTAATA803.7589797E-410.6905252
CTAATAC803.7589797E-410.6905253
TTTAGCC1106.0185994E-610.366573
TAGGACC1856.002665E-1110.2731784
GTATAAA2151.8189894E-1210.1928521
GTAGAAC2250.010.1362023
TGTACCG750.002640332610.1362025
GGTATAG856.4131804E-410.0885761
GCCTCGA856.608215E-410.05576216
AATCCCG951.6471547E-49.99740819
GCACTGT1856.493792E-109.7542296
AGGACGT5100.09.6890165
ATAGGGG800.00450318739.5026893
TAAGGTG1106.80877E-59.5026895
Produced by FastQC (version 0.11.5)