FastQCFastQC Report
Thu 26 May 2016
SRR1512066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650192
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22750.34989664591382236No Hit
TATCAACGCAGAGTACTTTTTTTTT14450.22224204542658169No Hit
GGTATCAACGCAGAGTACTTTTTTT14350.22070403819179565No Hit
GTCCTACAGTGGACATTTCTAAATT9940.15287791913773163No Hit
CTGTAGGACGTGGAATATGGCAAGA9420.14488028151684426No Hit
GTCCTAAAGTGTGTATTTCTCATTT9000.13842065113074292No Hit
CTTTAGGACGTGAAATATGGCGAGG7590.11673474912025986No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7000.10766050643502226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG801.3475983E-714.19416618
CCAACGA707.546867E-613.51825319
GTTTAGA603.066009E-413.1048271
GTATAAC450.009182801513.1048271
GTCTATA550.0023751312.5091531
TAGGACC2000.012.301614
GGTATCA3450.011.6803891
TCTAGGA853.754357E-511.5630832
CCCTTAG600.00455514411.4667231
GTTCAAG600.00455514411.4667231
GGCGAGG2650.011.42675119
AGGACGT5500.011.1832835
GGACGTG5450.011.1122536
CCACCTT2050.011.07837513
GTGTAGG802.7477863E-411.0571991
TAATGCT951.4170397E-510.9569014
GCACAGT700.001538957810.8146036
TGTAATA1001.6427197E-510.8114812
ACAGATA909.8800774E-510.5141978
CCAAGAC909.8800774E-510.5141973
Produced by FastQC (version 0.11.5)