FastQCFastQC Report
Thu 26 May 2016
SRR1512066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650192
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37420.5755223072569333No Hit
TATCAACGCAGAGTACTTTTTTTTT30890.4750904348254054No Hit
GGTATCAACGCAGAGTACTTTTTTT29480.4534045328149223No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13880.2134754041883013No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10440.1605679553116618No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9690.14903290105076653No Hit
CTGTAGGACGTGGAATATGGCAAGA8040.123655781676797No Hit
CTTTAGGACGTGAAATATGGCGAGG6710.10320028545414278No Hit
GGTATCAACGCAGAGTACATGGGGG6580.10120087604892093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG453.5397956E-516.8855419
GGTATCA7250.014.1892971
TAGGACC1900.014.0036854
GATATAC1301.8735591E-1012.45605851
AGGACCT3650.011.9757545
CTACACT657.994734E-411.6953854
CACCTTT1800.011.60791614
ACTGAGC752.07558E-411.3968628
GGACCTG3750.011.1435996
GACGTGG2650.011.1101497
ATAGGCT951.35276005E-511.0028954
ATGTCCA1658.0035534E-1110.9363829
TGTAGGA6400.010.838792
ACTGTTC1158.815932E-710.7361768
TGTCCAC1951.8189894E-1210.71499910
CTTTGAC803.7551718E-410.6903133
AGTACTA803.7551718E-410.6903135
ACCTTTT2050.010.65560215
GGACAGC1252.2391032E-710.6370726
CTGTAGG6400.010.5670421
Produced by FastQC (version 0.11.5)