##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 650192 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.662911878337475 31.0 28.0 31.0 16.0 34.0 2 27.829471909835863 31.0 27.0 31.0 16.0 34.0 3 27.77861616261043 31.0 27.0 31.0 16.0 34.0 4 31.193447473976917 35.0 31.0 37.0 19.0 37.0 5 30.549757610059796 35.0 30.0 37.0 10.0 37.0 6 30.579411927553707 35.0 30.0 37.0 11.0 37.0 7 30.26449879420233 35.0 30.0 37.0 10.0 37.0 8 30.090267182616827 35.0 30.0 37.0 10.0 37.0 9 31.06609586091493 35.0 30.0 39.0 8.0 39.0 10 30.662968784605162 35.0 30.0 39.0 2.0 39.0 11 30.772174988311146 35.0 30.0 39.0 2.0 39.0 12 30.383320619140193 35.0 29.0 39.0 2.0 39.0 13 30.38306530993922 35.0 29.0 39.0 2.0 39.0 14 30.762653185520584 36.0 28.0 39.0 2.0 41.0 15 30.660097325097816 36.0 28.0 39.0 2.0 40.0 16 30.458887528606933 36.0 27.0 39.0 2.0 40.0 17 30.365595085759285 36.0 27.0 39.0 2.0 40.0 18 30.210640856854592 36.0 27.0 39.0 2.0 40.0 19 30.229406083126214 36.0 27.0 39.0 2.0 40.0 20 30.03783959199744 36.0 27.0 39.0 2.0 40.0 21 29.82497785269582 36.0 27.0 39.0 2.0 40.0 22 29.588373895710806 36.0 27.0 39.0 2.0 40.0 23 29.541366242586804 36.0 27.0 39.0 2.0 40.0 24 29.252133216034647 36.0 27.0 39.0 2.0 40.0 25 28.662581206781997 35.0 25.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.024835563538260885 1101 2 -0.11726327177903073 1101 3 0.17556002563968676 1101 4 0.12867708799291222 1101 5 0.20421851587591178 1101 6 -0.14923934714378362 1101 7 -0.44210585516337275 1101 8 -0.1160501462186545 1101 9 -0.42348172719915667 1101 10 -0.12834404825377987 1101 11 -0.013494509736620586 1101 12 -0.29979604793352266 1101 13 -0.077823141211244 1101 14 -0.17647045322802413 1101 15 0.05561678635915612 1101 16 0.12281827399537804 1101 17 -0.5015055337231367 1101 18 -0.25584686006755675 1101 19 -0.17304077285741215 1101 20 -0.07987197355712539 1101 21 -0.17390604631628648 1101 22 -0.16951684207085904 1101 23 0.09760114396750552 1101 24 -0.4391759367826076 1101 25 -0.6694204139705207 1102 1 0.4573135113020257 1102 2 0.5357327312531517 1102 3 0.6132473835061347 1102 4 0.52222120969369 1102 5 0.5911125100871999 1102 6 0.69909122966002 1102 7 0.5585445135224525 1102 8 0.3801531650624419 1102 9 0.7291029499423232 1102 10 0.5427912112045625 1102 11 0.4371162338372194 1102 12 0.5434327957023406 1102 13 0.6643272102462738 1102 14 0.6845466200267474 1102 15 0.86456560105146 1102 16 0.3156891995001665 1102 17 0.40184149611629394 1102 18 0.08345823606640934 1102 19 0.17944182654993313 1102 20 0.41507367112621907 1102 21 0.5161545111918109 1102 22 0.6514659468478925 1102 23 0.7566012128807422 1102 24 0.6536545021947191 1102 25 0.3221269466524568 1103 1 -0.00535877915608296 1103 2 0.07194104242528354 1103 3 0.09190872323838661 1103 4 0.15290633097215434 1103 5 0.15925768966508613 1103 6 0.18648511358067665 1103 7 0.1169346413771244 1103 8 0.32609220217369383 1103 9 0.09751695004952055 1103 10 -0.12207369823342873 1103 11 -0.14813533187744454 1103 12 -0.034978689366162996 1103 13 0.08432836469026483 1103 14 0.28528422727665514 1103 15 0.38756377455614555 1103 16 0.6294320306091343 1103 17 0.653269089801487 1103 18 0.37361742564672795 1103 19 0.17153594671930605 1103 20 0.38475714732199506 1103 21 0.7482678446076036 1103 22 0.8225339312299127 1103 23 0.49534229170865274 1103 24 0.7404893053826349 1103 25 0.3873179865178784 1104 1 -0.6223983300191627 1104 2 -0.9222352605745492 1104 3 -0.7279588819233389 1104 4 -0.9818096066849584 1104 5 -1.1468844253005592 1104 6 -0.9261301034463045 1104 7 -1.0487935302334002 1104 8 -1.0081544879553483 1104 9 -0.7571126795801106 1104 10 -1.2178923675815092 1104 11 -1.1204622968514677 1104 12 -1.4388541711828218 1104 13 -1.3810269227238479 1104 14 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25 -1.013100591320054 1106 1 -0.7562043784415025 1106 2 -0.6249491841826256 1106 3 -0.33695722122934413 1106 4 -0.7216444113349034 1106 5 -0.4892714544064063 1106 6 -0.6268184174048734 1106 7 -0.4180619508260044 1106 8 -0.7281658505984758 1106 9 -0.6338957405013588 1106 10 -0.7219348463858104 1106 11 -0.8451694902786144 1106 12 -0.8689935820734753 1106 13 -1.1599514048495365 1106 14 -0.8325302954412841 1106 15 -1.5901966467406794 1106 16 -1.1617462204502473 1106 17 -1.269378154539666 1106 18 -1.4711076857945784 1106 19 -1.1076176496705443 1106 20 -1.20007159501046 1106 21 -1.6086576029567752 1106 22 -1.2190750568872488 1106 23 -0.7903030518861698 1106 24 -1.0732460209827082 1106 25 -1.2770563546766702 1107 1 0.07420350659177188 1107 2 0.07167879989500037 1107 3 -0.17270028363866174 1107 4 -0.2643488079324676 1107 5 -0.1964166093081836 1107 6 -0.035653191031851605 1107 7 -0.4799985895767236 1107 8 -0.2171945988450652 1107 9 -0.2942629004932158 1107 10 -0.18932527063964244 1107 11 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22 0.3291559378519189 1108 23 -0.16162236525600449 1108 24 0.08090664579997409 1108 25 0.11381371301360588 1109 1 -0.2129527132074145 1109 2 -0.4012425585610089 1109 3 -0.40624517401865035 1109 4 -0.5547323183705295 1109 5 -0.5220053256786485 1109 6 0.13361886340501172 1109 7 -0.25009485793937003 1109 8 -0.31805073344906276 1109 9 -0.11133540005843656 1109 10 -0.4908854213052436 1109 11 -0.4407713621816356 1109 12 -0.1888695585036686 1109 13 -0.21113111686787178 1109 14 -0.5821732612570649 1109 15 -0.6022347511168178 1109 16 -0.6461866373862151 1109 17 -0.3874026616325956 1109 18 -0.2848280153099232 1109 19 -0.44522206689969224 1109 20 -0.2731708738347258 1109 21 -0.6002686877854302 1109 22 -0.48183666910481193 1109 23 -0.3468255056160956 1109 24 -0.1847850548693053 1109 25 -0.3049705420935176 1110 1 -0.5995655126816999 1110 2 -0.6583033720296569 1110 3 -0.8089186342453196 1110 4 -0.5200449008026062 1110 5 -0.9897636822665028 1110 6 -0.6853406297620914 1110 7 -0.6723229419981038 1110 8 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-0.4690445419489784 2315 7 -0.18816766372518146 2315 8 -0.6204242443427539 2315 9 -0.8132574607248912 2315 10 -0.33212290828263846 2315 11 -0.357019040761152 2315 12 -0.6828678372553085 2315 13 -0.8669795866176884 2315 14 -0.9151819731895436 2315 15 -0.7754768884845156 2315 16 -1.0608036596265578 2315 17 -0.48853355200115445 2315 18 -0.6145980625687599 2315 19 -0.5692047940214344 2315 20 -0.6324663699015218 2315 21 -0.6270235306837719 2315 22 -0.606370497674515 2315 23 -0.6959293995630382 2315 24 -0.5016759367826076 2315 25 -0.47709537789548406 2316 1 -0.8265207986787573 2316 2 -1.089882319398079 2316 3 -1.1370717073813914 2316 4 -1.0934296645441677 2316 5 -1.5571544349437616 2316 6 -1.2074946164646256 2316 7 -1.5917057770993601 2316 8 -1.6977880462967185 2316 9 -2.035391368104701 2316 10 -1.9838036501273173 2316 11 -1.9963301849903274 2316 12 -1.977933245435473 2316 13 -1.771456045192508 2316 14 -1.6375311479977377 2316 15 -1.1981351652411547 2316 16 -2.25793894692578 2316 17 -1.8928940973453088 2316 18 -1.8427760169762237 2316 19 -1.9072132943250146 2316 20 -1.8594631132917065 2316 21 -2.0469495670032494 2316 22 -2.159900331532217 2316 23 -1.8766767639169615 2316 24 -1.6896540788591103 2316 25 -1.67726096822501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41961.0 3 2.0 4 601.0 5 4505.0 6 5414.0 7 4403.0 8 3664.0 9 3368.0 10 2938.0 11 2834.0 12 2771.0 13 2571.0 14 2540.0 15 2525.0 16 2692.0 17 2709.0 18 2929.0 19 3458.0 20 4108.0 21 5145.0 22 6450.0 23 7801.0 24 9079.0 25 10491.0 26 12147.0 27 13704.0 28 15664.0 29 18689.0 30 22676.0 31 27984.0 32 34322.0 33 41555.0 34 50247.0 35 61834.0 36 73780.0 37 82478.0 38 60153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.428084236970875 24.126852347657284 16.053911167555775 18.39115224781607 2 18.831975785614098 25.727016019883358 37.24392179540813 18.197086399094424 3 21.827244875359895 28.861936166547725 30.686935551344835 18.623883406747545 4 17.63463715333317 21.7711076112902 36.19915348081798 24.395101754558652 5 13.576086588559699 40.97169007105949 34.515709102323825 10.93651423805699 6 30.3385769126658 40.767803971749885 17.376713340059553 11.516905775524767 7 27.21442281664493 34.37369269385043 22.65115535103477 15.760729138469868 8 25.73680729655321 38.84291800605995 20.15918913514927 15.26108556223757 9 27.635375396805866 16.168762457858602 20.635904471294634 35.5599576740409 10 16.24151020006398 31.69033147131924 33.22895390899919 18.839204419617587 11 34.78557103132613 23.780667864261634 24.561975539532938 16.871785564879296 12 23.610871865541256 27.101686886335113 31.406107734330785 17.88133351379285 13 30.303356546989196 23.299425400497086 25.982479021581316 20.414739030932402 14 21.98504441764894 23.98891404385166 28.113388045377363 25.912653493122033 15 23.96369072520117 32.31614661515368 23.749600118118956 19.970562541526196 16 22.620856608509488 29.953921303245814 27.44635430765066 19.97886778059404 17 22.04148928316559 30.289360681152644 27.787484312326207 19.881665723355564 18 21.884766345940893 28.728744740015255 30.117872874474 19.26861603956985 19 24.005290761162122 28.04072655685261 28.846952429289903 19.107030252695367 20 24.09595934739277 28.36808204345793 28.927916676920045 18.60804193222925 21 23.788204099712086 27.583544552993576 28.456978861628563 20.171272485665774 22 23.88233014248099 28.485893397642542 28.709212048133477 18.922564411742993 23 23.098407854910548 28.521113763319146 28.830099416787657 19.550378964982652 24 23.289369458052313 28.275411248086996 29.206420007536664 19.22879928632403 25 23.31093967246195 28.36135616994574 29.08115241224023 19.246551745352086 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 683.0 1 683.0 2 642.0 3 601.0 4 601.0 5 601.0 6 1127.5 7 1654.0 8 1654.0 9 1654.0 10 2204.5 11 2755.0 12 2755.0 13 2755.0 14 3792.5 15 4830.0 16 4830.0 17 4830.0 18 8265.0 19 11700.0 20 11700.0 21 11700.0 22 17750.5 23 23801.0 24 23801.0 25 23801.0 26 33121.5 27 42442.0 28 42442.0 29 42442.0 30 51122.5 31 59803.0 32 59803.0 33 59803.0 34 65286.5 35 70770.0 36 70770.0 37 70770.0 38 72994.5 39 75219.0 40 75219.0 41 75219.0 42 76840.5 43 78462.0 44 78462.0 45 78462.0 46 77513.5 47 76565.0 48 76565.0 49 76565.0 50 72878.0 51 69191.0 52 69191.0 53 69191.0 54 60639.5 55 52088.0 56 52088.0 57 52088.0 58 43906.0 59 35724.0 60 35724.0 61 35724.0 62 28898.0 63 22072.0 64 22072.0 65 22072.0 66 17170.5 67 12269.0 68 12269.0 69 12269.0 70 9040.0 71 5811.0 72 5811.0 73 5811.0 74 4137.5 75 2464.0 76 2464.0 77 2464.0 78 1688.0 79 912.0 80 912.0 81 912.0 82 595.5 83 279.0 84 279.0 85 279.0 86 178.5 87 78.0 88 78.0 89 78.0 90 47.0 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3326709648842188 2 0.0 3 0.0 4 0.0 5 0.04829342717228142 6 0.0 7 0.0 8 0.0033836159165292713 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.076014469572065E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00569062676870832 25 0.001230405787828826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 650192.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.61049614826602 #Duplication Level Percentage of deduplicated Percentage of total 1 90.89186566657054 73.26838387223796 2 6.410660831426209 10.335331005190444 3 1.422533506356688 3.4401339520483547 4 0.5001496349284235 1.6126924087981733 5 0.23339234552973923 0.9406936385179907 6 0.13364328590957136 0.6463830950433072 7 0.08861235558808724 0.5000160164175599 8 0.05420340398310001 0.3495490630402073 9 0.04022239960778189 0.29181128297914066 >10 0.19013985853714369 2.704692834995538 >50 0.01891224689437729 1.0377524964063458 >100 0.013372103985543287 2.26858475598198 >500 0.0013372103983166606 0.723600310031559 >1k 9.551502845119004E-4 1.880375268311421 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3742 0.5755223072569333 No Hit TATCAACGCAGAGTACTTTTTTTTT 3089 0.4750904348254054 No Hit GGTATCAACGCAGAGTACTTTTTTT 2948 0.4534045328149223 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1388 0.2134754041883013 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1044 0.1605679553116618 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 969 0.14903290105076653 No Hit CTGTAGGACGTGGAATATGGCAAGA 804 0.123655781676797 No Hit CTTTAGGACGTGAAATATGGCGAGG 671 0.10320028545414278 No Hit GGTATCAACGCAGAGTACATGGGGG 658 0.10120087604892093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5380072347860325E-4 0.0 10 0.0 0.0 0.0 4.6140217043580975E-4 0.0 11 0.0 0.0 0.0 4.6140217043580975E-4 0.0 12 0.0 0.0 0.0 4.6140217043580975E-4 0.0 13 0.0 0.0 0.0 4.6140217043580975E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 45 3.5397956E-5 16.88554 19 GGTATCA 725 0.0 14.189297 1 TAGGACC 190 0.0 14.003685 4 GATATAC 130 1.8735591E-10 12.4560585 1 AGGACCT 365 0.0 11.975754 5 CTACACT 65 7.994734E-4 11.695385 4 CACCTTT 180 0.0 11.607916 14 ACTGAGC 75 2.07558E-4 11.396862 8 GGACCTG 375 0.0 11.143599 6 GACGTGG 265 0.0 11.110149 7 ATAGGCT 95 1.35276005E-5 11.002895 4 ATGTCCA 165 8.0035534E-11 10.936382 9 TGTAGGA 640 0.0 10.83879 2 ACTGTTC 115 8.815932E-7 10.736176 8 TGTCCAC 195 1.8189894E-12 10.714999 10 CTTTGAC 80 3.7551718E-4 10.690313 3 AGTACTA 80 3.7551718E-4 10.690313 5 ACCTTTT 205 0.0 10.655602 15 GGACAGC 125 2.2391032E-7 10.637072 6 CTGTAGG 640 0.0 10.567042 1 >>END_MODULE