FastQCFastQC Report
Thu 26 May 2016
SRR1512065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663422
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61600.37032094080756417No Hit
GGTATCAACGCAGAGTACTTTTTTT38660.23241246057825374No Hit
TATCAACGCAGAGTACTTTTTTTTT34390.20674248627227487No Hit
GTCCTACAGTGGACATTTCTAAATT24830.14927060000408796No Hit
GTCCTAAAGTGTGTATTTCTCATTT24180.14536299267413802No Hit
CTGTAGGACGTGGAATATGGCAAGA23080.1387501187311458No Hit
CTTTAGGACGTGAAATATGGCGAGG22870.13748766097839274No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17530.10538516383695778No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17310.10406258904835934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.5428721
ACTCGCG500.001547297113.244878
TCCAACG2050.012.921823518
GGACCGT550.003165543812.0407916
AATACCG550.003165543812.0407915
TGCCGGT1751.8189894E-1211.35240610
GTGCGCC752.156139E-411.35240511
CCGGTTT1604.9112714E-1111.2341512
CTCGAAC1451.3042154E-911.091763518
TGGACGG600.006060417711.0373925
TCGAACT1303.4715413E-810.91610119
GACCGTG700.001544073210.81213957
TAGGACC3500.010.81213954
GGCGAGG6000.010.72203719
GCCGGTT1605.857146E-1010.642879511
GCCTCGA1252.3716166E-710.59589716
GCGTACA854.7281044E-410.4287361
ACTGTTC5000.010.4066848
CCTCGAA1553.919922E-910.37616517
GTCTTAG2001.2732926E-1110.341831
Produced by FastQC (version 0.11.5)