FastQCFastQC Report
Thu 26 May 2016
SRR1512065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663422
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77370.46512550633573435No Hit
TATCAACGCAGAGTACTTTTTTTTT52710.316876895941018No Hit
GGTATCAACGCAGAGTACTTTTTTT52710.316876895941018No Hit
GTCCTAAAGTGTGTATTTCTCATTT25050.15059317479268639No Hit
GTCCTACAGTGGACATTTCTAAATT24900.14969141925500565No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24560.1476474400362626No Hit
CTGTAGGACGTGGAATATGGCAAGA23400.14067386387819808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23320.140192927591435No Hit
CTTTAGGACGTGAAATATGGCGAGG22550.13556391583134045No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17150.1031007164748332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.013.4210791
TAGGACC3400.013.1335794
TCTATAC1207.385097E-1012.667853
CTGTAGG14650.09.957461
GGCGAGG7250.09.95625819
AGGACGG1151.0251393E-59.9139695
TGTAGGA15550.09.8980312
AGAACAG6250.09.8809225
AGGACGT15900.09.7996565
TCGTCAA1651.0264557E-89.78496710
GGACGTG15750.09.7688166
CTAGGAC1856.475602E-109.7576683
GAACAGT4700.09.6995336
TATACAC6850.09.5702363
TAGAACA7950.09.560644
AGGACCT7950.09.560645
CTATACA2303.6379788E-129.5008874
AGGACTG3400.09.5008875
ACTGTTC5100.09.497468
GACTATC800.0045257029.497467
Produced by FastQC (version 0.11.5)