##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512065_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663422 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.660096475819124 31.0 30.0 34.0 26.0 34.0 2 29.87278393576615 31.0 30.0 34.0 26.0 34.0 3 29.93167278056921 31.0 30.0 34.0 26.0 34.0 4 33.52230883083186 35.0 35.0 37.0 28.0 37.0 5 33.02692341450336 35.0 33.0 37.0 26.0 37.0 6 32.97111737129844 35.0 33.0 37.0 26.0 37.0 7 32.73210526252508 35.0 33.0 37.0 26.0 37.0 8 32.602004181741016 35.0 33.0 37.0 25.0 37.0 9 33.916059184019446 37.0 33.0 39.0 25.0 39.0 10 33.64197539770425 37.0 33.0 39.0 25.0 39.0 11 33.656469615046575 37.0 33.0 39.0 24.0 39.0 12 33.406818594439656 37.0 33.0 39.0 23.0 39.0 13 33.38503759118252 37.0 33.0 39.0 22.0 39.0 14 34.15621832583674 38.0 33.0 40.0 20.0 41.0 15 34.0600719480685 38.0 33.0 40.0 19.0 41.0 16 33.935076607138775 38.0 33.0 40.0 18.0 41.0 17 33.85461356168188 38.0 33.0 40.0 18.0 41.0 18 33.758740716426736 38.0 33.0 40.0 17.0 41.0 19 33.77914744424446 38.0 33.0 40.0 18.0 41.0 20 33.63627089217288 38.0 33.0 40.0 18.0 41.0 21 33.49170384905334 38.0 33.0 40.0 17.0 41.0 22 33.32507325260818 38.0 32.0 40.0 11.0 41.0 23 33.358777267584536 38.0 33.0 40.0 10.0 41.0 24 33.17307995205065 38.0 33.0 40.0 2.0 41.0 25 32.67760075314622 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.004881782120136791 1101 2 -0.005892454889654175 1101 3 0.04341069924610963 1101 4 0.10523136022664659 1101 5 0.11735702266752668 1101 6 -0.1386629835834583 1101 7 -0.23779487025031187 1101 8 -0.32602157782864793 1101 9 -0.1246853606735101 1101 10 -0.11987345856422849 1101 11 -0.24347233856761363 1101 12 -0.02334553524794103 1101 13 -0.16642959862594608 1101 14 -0.15628953121859723 1101 15 0.0939591010954004 1101 16 -0.19507374335575633 1101 17 -0.2612760181451108 1101 18 -0.1299921932417618 1101 19 -0.08294317440392973 1101 20 -0.13337877591536795 1101 21 -0.09692350175779296 1101 22 -0.5558183908170378 1101 23 -0.2034745514575178 1101 24 -0.7715990220395028 1101 25 -0.8624430238997931 1102 1 0.20638038973255135 1102 2 0.2092509649392973 1102 3 0.22882390882323378 1102 4 0.1900693144458856 1102 5 0.16482454447812955 1102 6 0.2045371112395742 1102 7 0.3793750661609536 1102 8 0.25398697968907413 1102 9 0.2916176874700156 1102 10 0.3143850722519588 1102 11 0.27504876085849617 1102 12 0.43193166580552855 1102 13 0.38902200906722584 1102 14 0.3079756684698438 1102 15 0.506808858697525 1102 16 0.5641115861672859 1102 17 0.6485085981212109 1102 18 0.5982545185613617 1102 19 0.4312281791426855 1102 20 0.6654495475145197 1102 21 0.8085683536214603 1102 22 0.6361930296267033 1102 23 0.7310207112986546 1102 24 0.770421385751618 1102 25 0.7438688292391333 1103 1 0.19831678124942087 1103 2 0.41162933249458433 1103 3 0.3509334873035286 1103 4 0.3188036075306826 1103 5 0.29078148223903355 1103 6 0.2904757097193098 1103 7 0.456467958509819 1103 8 0.369449206413222 1103 9 0.3645775460611489 1103 10 0.38226336854803833 1103 11 0.2617279130302137 1103 12 0.413754033033058 1103 13 0.3032925651153775 1103 14 0.47558847242541447 1103 15 0.3211934613533387 1103 16 0.39726174987215046 1103 17 0.5143334178131056 1103 18 0.5151723460197175 1103 19 0.6456807570200667 1103 20 0.502945725502471 1103 21 0.7019135730535737 1103 22 0.5049367665485676 1103 23 0.5425221060341485 1103 24 0.8569173045282454 1103 25 0.42660532291014164 1104 1 0.05620969868574477 1104 2 0.04008943531973941 1104 3 0.08918036534684504 1104 4 0.11784353582694251 1104 5 0.056923681249472224 1104 6 -0.03127758460212959 1104 7 0.004251778629956959 1104 8 0.10664274426691378 1104 9 0.23633816588482404 1104 10 0.09187567232751803 1104 11 0.2607923554254299 1104 12 0.2755165392932497 1104 13 0.229711964960444 1104 14 0.14606918816049586 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2315 4 -0.7569325629861225 2315 5 -0.7885119932565914 2315 6 -0.6563141094167406 2315 7 -0.7177544629041641 2315 8 -0.9371826883009433 2315 9 -0.9405820617475982 2315 10 -0.8173700753150683 2315 11 -0.5654375124769402 2315 12 -0.6133078993651537 2315 13 -0.6043160187503034 2315 14 -0.9083655489205782 2315 15 -0.7600183173749286 2315 16 -0.8370559588163928 2315 17 -1.1746080836813704 2315 18 -0.9985518578357215 2315 19 -0.9942725628245199 2315 20 -0.892680499824543 2315 21 -0.7419148925311774 2315 22 -0.8164981069484298 2315 23 -0.9198344407169117 2315 24 -0.9233720214999508 2315 25 -0.9665136844220648 2316 1 -0.9527864121476775 2316 2 -1.1297152437153208 2316 3 -1.1911269417713974 2316 4 -1.1001762345379618 2316 5 -1.1448980111936642 2316 6 -1.3514581537958001 2316 7 -1.514811955213446 2316 8 -1.580288215982609 2316 9 -1.8222132526122508 2316 10 -1.980244175639843 2316 11 -1.8187225092189827 2316 12 -1.9395327022653532 2316 13 -1.8891342315626503 2316 14 -1.9761677243817743 2316 15 -1.6207808573817957 2316 16 -1.885932070150993 2316 17 -1.865205531342621 2316 18 -1.8668244224159203 2316 19 -1.912635945300579 2316 20 -1.7028760129210774 2316 21 -2.02854789031608 2316 22 -1.9790705951228347 2316 23 -1.920863146564848 2316 24 -2.073882776312864 2316 25 -1.7438134180831995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50701.0 3 1.0 4 819.0 5 7139.0 6 8425.0 7 7088.0 8 5604.0 9 5027.0 10 4738.0 11 4447.0 12 4195.0 13 4073.0 14 3995.0 15 3918.0 16 4141.0 17 4305.0 18 4592.0 19 5270.0 20 6328.0 21 8127.0 22 10156.0 23 12786.0 24 15601.0 25 17950.0 26 20677.0 27 23627.0 28 27298.0 29 32740.0 30 41052.0 31 52005.0 32 65664.0 33 82685.0 34 106150.0 35 138544.0 36 194171.0 37 296887.0 38 382496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.59231430420212 22.194945578165594 14.878480438078867 22.334259679553423 2 15.438595858417168 23.425144070476403 40.093133311931666 21.04312675917476 3 18.732408252385742 27.83773450152757 31.255989159696096 22.173868086390584 4 13.545029463359267 19.583184543669617 38.65585521894023 28.215930774030884 5 12.989349422780458 38.88047137540834 35.896120331613275 12.234058870197932 6 30.052566336143204 39.49009932536662 18.27611995031928 12.181214388170892 7 26.032600266198237 33.331890524473046 23.730117793320034 16.905391416008687 8 24.29762818000149 38.050420713686236 21.32617995820518 16.325771148107087 9 26.314188462098016 15.493843414359073 21.316418804127878 36.875549319415036 10 15.086851081685825 30.165586363532526 34.563868940052494 20.183693614729155 11 33.552459928989755 23.521211093757326 25.122668811642505 17.80366016561041 12 22.459724591835382 26.171650970108608 32.79143837222304 18.57718606583296 13 28.431690815679968 23.306713509860995 26.678978635607802 21.582617038851236 14 20.666673880710967 23.00835266096036 28.852389832525958 27.47258362580271 15 22.717746909683772 31.70193733159715 24.310668008478906 21.269647750240168 16 21.78455016225588 29.412500255497402 28.186894245717564 20.616055336529154 17 20.789432867907244 29.63102568079537 28.88785888367474 20.69168256762265 18 20.584554009746174 28.635788152375042 30.908693043617312 19.870964794261468 19 22.677241660409482 27.3811620750249 29.173071639711175 20.768524624854443 20 22.979256015611192 27.95165628445457 29.325751372772512 19.743336327161717 21 22.861366508318394 27.630511078968535 28.173668497831578 21.33445391488149 22 22.336123966137276 28.748808179764367 29.00857389165227 19.90649396244609 23 21.721307040546538 28.59508891910772 29.05877161658316 20.62483242376258 24 22.1909463872603 27.486689400097152 29.797046417560853 20.52531779508169 25 22.34457492638598 27.915373636983393 29.223893625488905 20.516157811141717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1046.0 1 1046.0 2 1027.0 3 1008.0 4 1008.0 5 1008.0 6 2268.0 7 3528.0 8 3528.0 9 3528.0 10 4705.0 11 5882.0 12 5882.0 13 5882.0 14 8893.5 15 11905.0 16 11905.0 17 11905.0 18 20007.5 19 28110.0 20 28110.0 21 28110.0 22 42288.0 23 56466.0 24 56466.0 25 56466.0 26 78832.5 27 101199.0 28 101199.0 29 101199.0 30 126509.0 31 151819.0 32 151819.0 33 151819.0 34 167181.0 35 182543.0 36 182543.0 37 182543.0 38 192778.5 39 203014.0 40 203014.0 41 203014.0 42 206480.0 43 209946.0 44 209946.0 45 209946.0 46 206117.5 47 202289.0 48 202289.0 49 202289.0 50 190570.5 51 178852.0 52 178852.0 53 178852.0 54 156118.0 55 133384.0 56 133384.0 57 133384.0 58 112015.0 59 90646.0 60 90646.0 61 90646.0 62 72585.0 63 54524.0 64 54524.0 65 54524.0 66 41227.0 67 27930.0 68 27930.0 69 27930.0 70 20170.0 71 12410.0 72 12410.0 73 12410.0 74 8566.0 75 4722.0 76 4722.0 77 4722.0 78 3178.0 79 1634.0 80 1634.0 81 1634.0 82 1030.5 83 427.0 84 427.0 85 427.0 86 271.0 87 115.0 88 115.0 89 115.0 90 67.0 91 19.0 92 19.0 93 19.0 94 11.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40482811938281443 2 0.0 3 0.0 4 0.0 5 0.05308334265147389 6 0.0 7 0.0 8 0.0034867880790322597 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.011703584538379E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0051700650827030065 25 9.618725735261407E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1663422.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.3355566815302 #Duplication Level Percentage of deduplicated Percentage of total 1 86.67803492573424 65.29938013191308 2 9.521528204762484 14.346192555293454 3 2.0801158524243295 4.701200571133878 4 0.6924209490381543 2.0865567061497123 5 0.3140976645638061 1.183136120614144 6 0.18521740064742334 0.8372073590927792 7 0.10826534903468926 0.5709361237193941 8 0.08094664629967799 0.4878528528391335 9 0.04999128128329215 0.33895089042297993 >10 0.2528513204876666 3.356406713108398 >50 0.018255207303877707 0.9554703434087501 >100 0.014779487230562424 2.2401681480180162 >500 0.002303991466093385 1.2062090286706932 >1k 9.533757790731247E-4 1.2962871603706583 >5k 2.3834394476828118E-4 1.0940452952449564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7737 0.46512550633573435 No Hit TATCAACGCAGAGTACTTTTTTTTT 5271 0.316876895941018 No Hit GGTATCAACGCAGAGTACTTTTTTT 5271 0.316876895941018 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2505 0.15059317479268639 No Hit GTCCTACAGTGGACATTTCTAAATT 2490 0.14969141925500565 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2456 0.1476474400362626 No Hit CTGTAGGACGTGGAATATGGCAAGA 2340 0.14067386387819808 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2332 0.140192927591435 No Hit CTTTAGGACGTGAAATATGGCGAGG 2255 0.13556391583134045 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1715 0.1031007164748332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.011703584538379E-5 0.0 8 0.0 0.0 0.0 6.011703584538379E-5 0.0 9 0.0 0.0 0.0 1.2023407169076758E-4 0.0 10 0.0 0.0 0.0 1.8035110753615138E-4 0.0 11 0.0 0.0 0.0 2.4046814338153516E-4 0.0 12 0.0 0.0 0.0 2.4046814338153516E-4 0.0 13 0.0 0.0 0.0 2.4046814338153516E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1570 0.0 13.421079 1 TAGGACC 340 0.0 13.133579 4 TCTATAC 120 7.385097E-10 12.66785 3 CTGTAGG 1465 0.0 9.95746 1 GGCGAGG 725 0.0 9.956258 19 AGGACGG 115 1.0251393E-5 9.913969 5 TGTAGGA 1555 0.0 9.898031 2 AGAACAG 625 0.0 9.880922 5 AGGACGT 1590 0.0 9.799656 5 TCGTCAA 165 1.0264557E-8 9.784967 10 GGACGTG 1575 0.0 9.768816 6 CTAGGAC 185 6.475602E-10 9.757668 3 GAACAGT 470 0.0 9.699533 6 TATACAC 685 0.0 9.570236 3 TAGAACA 795 0.0 9.56064 4 AGGACCT 795 0.0 9.56064 5 CTATACA 230 3.6379788E-12 9.500887 4 AGGACTG 340 0.0 9.500887 5 ACTGTTC 510 0.0 9.49746 8 GACTATC 80 0.004525702 9.49746 7 >>END_MODULE