FastQCFastQC Report
Thu 26 May 2016
SRR1512064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483676
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53150.35823185115887834No Hit
TATCAACGCAGAGTACTTTTTTTTT34610.2332719542541633No Hit
GGTATCAACGCAGAGTACTTTTTTT31800.214332509254042No Hit
CTGTAGGACGTGGAATATGGCAAGA26520.17874522469865387No Hit
GTCCTACAGTGGACATTTCTAAATT26020.17537521669151485No Hit
GTCCTAAAGTGTGTATTTCTCATTT25560.17227480932494696No Hit
CTTTAGGACGTGAAATATGGCGAGG23240.15663797217182188No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15210.10251564357716915No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14980.10096543989388518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1007.003109E-1014.19189318
CGCTGGA450.00911217713.121791
CCAACGA655.665194E-513.10020819
CGGTAGG550.002354729512.5253461
AGGACGG951.0922358E-611.9510675
GGTATCA10550.011.8469251
GGCGAGG7800.011.28073419
TCTAGAC1355.0386006E-911.21334653
AAATGTC5100.011.1308967
AGGACGT16750.010.9581185
GGACGGG951.4229592E-510.9551446
GGACGTG16950.010.8846376
GACGTGG8450.010.8608567
TAGGACC4400.010.7514334
AATGTCC5200.010.7352548
TGTCCAC4950.010.70400910
GAAATGT5600.010.6439186
TTAGACA909.915773E-510.5125114
AAGTCGT1803.8198777E-1110.5125117
TTAGGAC10500.010.3623333
Produced by FastQC (version 0.11.5)