FastQCFastQC Report
Thu 26 May 2016
SRR1512064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483676
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66540.44848066559006144No Hit
TATCAACGCAGAGTACTTTTTTTTT50810.3424602136854677No Hit
GGTATCAACGCAGAGTACTTTTTTT46750.31509574866749884No Hit
CTGTAGGACGTGGAATATGGCAAGA24960.16823079971638014No Hit
CTTTAGGACGTGAAATATGGCGAGG23050.15535736912910905No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22260.15003275647782938No Hit
GTCCTACAGTGGACATTTCTAAATT20330.13702452557027275No Hit
GTCCTAAAGTGTGTATTTCTCATTT20290.13675492492970165No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18580.12522949754528617No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15270.10292004453802582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG250.006022559519.0051275
ACACCGA604.1023153E-412.6628266
GGTATCA13400.012.3068291
AATCCCG907.479428E-611.60837219
GGATAAG951.30950575E-511.0375821
TGCCGGT803.782217E-410.68389910
CACCTTT5500.010.18747514
AGAACCG750.002641531610.1360675
CCACCTT5550.09.924582513
CTTATAC1151.022753E-59.9157183
TAGGACC4150.09.8460294
TGTAGGA15650.09.7757992
CTAGGAC1856.4392225E-109.7593893
ACCTTTT5900.09.65776115
GGCGAGG6950.09.56608819
GTATCAA25750.09.51394751
CTAGACC800.0045052079.5025633
TACACTA1606.601658E-89.5025635
CCTTATA1401.0539861E-69.50256252
CAAGTCG1304.25267E-69.4971196
Produced by FastQC (version 0.11.5)