##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512064_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1483676 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.76982103909479 31.0 28.0 31.0 23.0 34.0 2 28.977217397868536 31.0 28.0 33.0 24.0 34.0 3 28.992801662896753 31.0 28.0 34.0 24.0 34.0 4 32.54564338844869 35.0 32.0 37.0 26.0 37.0 5 31.97848519488082 35.0 32.0 37.0 22.0 37.0 6 31.93050099887037 35.0 32.0 37.0 22.0 37.0 7 31.65082538236111 35.0 32.0 37.0 19.0 37.0 8 31.490891542358305 35.0 32.0 37.0 17.0 37.0 9 32.648934807869104 37.0 32.0 39.0 17.0 39.0 10 32.32088340041896 37.0 32.0 39.0 17.0 39.0 11 32.36445625594806 37.0 32.0 39.0 17.0 39.0 12 32.062256853922285 37.0 32.0 39.0 11.0 39.0 13 32.03405325691054 37.0 32.0 39.0 11.0 39.0 14 32.62345080731912 37.0 32.0 40.0 10.0 41.0 15 32.52390616280104 37.0 31.0 40.0 10.0 41.0 16 32.356692431501216 37.0 31.0 40.0 10.0 41.0 17 32.27702139820284 37.0 31.0 40.0 10.0 41.0 18 32.14143788805642 37.0 31.0 40.0 8.0 41.0 19 32.16108907874765 37.0 31.0 40.0 2.0 41.0 20 31.989160032244236 37.0 31.0 40.0 2.0 41.0 21 31.808989294158565 37.0 31.0 40.0 2.0 41.0 22 31.619527443997207 37.0 31.0 40.0 2.0 41.0 23 31.619577994117314 37.0 31.0 40.0 2.0 41.0 24 31.39025231923951 37.0 31.0 40.0 2.0 41.0 25 30.828565670672035 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17762553164292427 1101 2 0.126243751222205 1101 3 0.0554529851662835 1101 4 0.06690112506939982 1101 5 0.2559879741034159 1101 6 0.1463703492304944 1101 7 0.023443126167766337 1101 8 0.04969384169388391 1101 9 0.31294955416571213 1101 10 0.5665747277250333 1101 11 0.507489606916586 1101 12 0.3952554047835264 1101 13 0.34694894829102907 1101 14 0.13740737919454915 1101 15 0.5442524486940243 1101 16 0.2235312773518956 1101 17 0.5364416349749774 1101 18 0.43630112330799875 1101 19 0.526606670286526 1101 20 0.4250976105179838 1101 21 0.41510946259063886 1101 22 -0.02306539327875612 1101 23 0.22712159992886782 1101 24 -0.34838336287072735 1101 25 -0.31643756202116435 1102 1 0.5220950226679015 1102 2 0.5497907950129921 1102 3 0.7512608392037698 1102 4 0.6612646262962798 1102 5 0.17030830561425958 1102 6 0.315096333530569 1102 7 0.7506251822273065 1102 8 0.5984965947814374 1102 9 0.8645465282129763 1102 10 0.6159531449033224 1102 11 0.5914013533555931 1102 12 0.7284820356111865 1102 13 1.0797854226284684 1102 14 0.9897628777334972 1102 15 0.8054263798872014 1102 16 0.9419751312672062 1102 17 0.8593478528395053 1102 18 0.7136184919916246 1102 19 0.5739842118526539 1102 20 0.9260968265396521 1102 21 1.0208359661490896 1102 22 0.8570812208511782 1102 23 1.0042726023011674 1102 24 0.9894697582793839 1102 25 0.7050234885098021 1103 1 -0.003727867891832659 1103 2 0.1443438443028846 1103 3 0.08287320344771487 1103 4 0.28085526957112705 1103 5 0.2122464843945373 1103 6 -0.13658286446441892 1103 7 0.189230027674256 1103 8 0.30993352042888844 1103 9 0.3117478648879981 1103 10 0.13261981156999525 1103 11 0.033999515427439064 1103 12 0.2102531300138608 1103 13 0.08062084451652396 1103 14 0.34456655358980015 1103 15 0.2803470148078375 1103 16 0.14005366092468563 1103 17 0.3106176941093466 1103 18 0.46843887628569547 1103 19 0.3125138693296776 1103 20 0.22873675970589957 1103 21 0.2944449887054752 1103 22 0.3215674363900298 1103 23 0.06250986211736986 1103 24 0.31489164633284616 1103 25 0.199016805134697 1104 1 -0.01919692026961073 1104 2 0.007130557922376823 1104 3 0.06676013596888808 1104 4 0.08421017582410428 1104 5 0.03202218948003832 1104 6 0.11197999838891448 1104 7 0.3634963897820569 1104 8 0.16297117786361426 1104 9 0.3258083325127501 1104 10 0.03596520035841877 1104 11 0.370874931816509 1104 12 0.40722023131140617 1104 13 0.2682925554394018 1104 14 0.20143657674896787 1104 15 0.3325169967246566 1104 16 0.5325316914440208 1104 17 0.3104971395584073 1104 18 0.5885270714571647 1104 19 0.6308397473157825 1104 20 0.27163831739759914 1104 21 0.4862488654860364 1104 22 0.3306074414660074 1104 23 0.5488094719013255 1104 24 0.47153325034287263 1104 25 0.7936029540512948 1105 1 0.09455302852396485 1105 2 0.05944713651399525 1105 3 -0.14966688463657363 1105 4 -0.10865369702010241 1105 5 0.1081415618941115 1105 6 -0.04248110521191961 1105 7 0.07294295075866586 1105 8 -0.14468725481018296 1105 9 -0.00649677584004138 1105 10 0.03943904411737975 1105 11 -0.1985077235437771 1105 12 -0.31063647263353644 1105 13 -0.18626481623730484 1105 14 0.2682957795673673 1105 15 0.06120693159008539 1105 16 0.10089021833763212 1105 17 0.09177811944452685 1105 18 -0.12789175612056525 1105 19 -0.17681868535801115 1105 20 0.09002347163134772 1105 21 -0.25884965567245644 1105 22 -0.348115265510355 1105 23 -0.3550863127859074 1105 24 0.047099593384942295 1105 25 0.13561680026816347 1106 1 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-1.3451840688612506 2315 13 -1.3310110989442414 2315 14 -1.685628564952598 2315 15 -1.6645340272849616 2315 16 -1.623463081194366 2315 17 -1.723822683398101 2315 18 -1.6369734865732255 2315 19 -1.6534872298765109 2315 20 -1.5519706606913601 2315 21 -1.5413931937891903 2315 22 -1.5161688598894791 2315 23 -1.7142393772206113 2315 24 -1.6379241724152287 2315 25 -1.7784697711402018 2316 1 -1.5922832059410972 2316 2 -1.6246311078704494 2316 3 -1.5892749924859082 2316 4 -1.780101486223682 2316 5 -1.8038440377688545 2316 6 -1.8008785889828154 2316 7 -1.752191573666373 2316 8 -2.1368546778775297 2316 9 -2.267551715423732 2316 10 -2.28316632536999 2316 11 -2.4724626458930246 2316 12 -2.4895084781485615 2316 13 -2.223410313259574 2316 14 -2.3393955706557215 2316 15 -2.5951813030281734 2316 16 -2.753986172386405 2316 17 -2.711501212624949 2316 18 -2.6587615211576185 2316 19 -2.439844096591038 2316 20 -2.1483246067279325 2316 21 -2.5067647664516457 2316 22 -2.549313538235886 2316 23 -2.7047689117403486 2316 24 -2.59947266681689 2316 25 -2.6875932110299736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64914.0 3 5.0 4 1023.0 5 8341.0 6 9789.0 7 8005.0 8 6470.0 9 5846.0 10 5461.0 11 5078.0 12 4919.0 13 4599.0 14 4529.0 15 4626.0 16 4600.0 17 4894.0 18 5247.0 19 5954.0 20 7043.0 21 9104.0 22 11522.0 23 14016.0 24 16878.0 25 19408.0 26 22563.0 27 25550.0 28 29523.0 29 35520.0 30 43311.0 31 54397.0 32 68073.0 33 85761.0 34 107539.0 35 140026.0 36 188304.0 37 248640.0 38 202198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.28488318604185 23.152339796840586 15.710557362499392 21.852219654618168 2 16.3825525249448 24.040221719566805 38.807394606369584 20.76983114911881 3 19.59754016375543 27.681650171600808 31.321932820912384 21.398876843731383 4 14.791032543493325 20.59849994203586 37.59021511435111 27.020252400119706 5 12.918063076124264 39.507205135639964 35.7153549970667 11.859376791169073 6 30.028321547292 39.66566824562775 18.142303306112655 12.163706900967597 7 26.476265707607322 33.32540258115653 23.366826719580285 16.83150499165586 8 24.748909599367497 38.03100370780027 20.92342492631244 16.296661766519797 9 26.403136533852408 15.698036498534721 21.0920039146013 36.80682305301157 10 15.15964401931419 30.46359178149407 34.49358215675121 19.883182042440534 11 33.42151520952014 23.22097277303131 25.51217381692499 17.845338200523564 12 23.04627155794122 26.11230484283631 32.09959586863979 18.74182773058269 13 28.766792749899572 23.079162836090898 26.498642560774726 21.655401853234803 14 20.824425278834465 23.466848557232172 28.504538726784013 27.20418743714935 15 22.61181012566086 32.31264777485111 24.10088186369531 20.97466023579272 16 21.583216281721885 29.387885225615296 28.340756337637057 20.68814215502576 17 21.14693504511767 29.910506067362412 28.410717703865263 20.531841183654652 18 20.928962927215917 28.382544436925585 30.81986902800881 19.868623607849692 19 23.017828690360968 27.80748627058738 28.953086792534222 20.221598246517434 20 23.132408962603694 28.22866987131961 29.194446766005516 19.444474400071172 21 22.60143050099887 27.510453764838143 28.547742229435535 21.34037350472745 22 22.88168036687255 28.650662273973566 28.746505301696594 19.72115205745729 23 21.80368220554892 28.6694669186534 28.9607704107905 20.566080465007186 24 22.5777707078496 27.83817922986913 29.419139493877022 20.164910568404252 25 22.252035841047356 28.33772112516193 29.176321830713466 20.23392120307725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 868.0 1 868.0 2 907.0 3 946.0 4 946.0 5 946.0 6 1949.5 7 2953.0 8 2953.0 9 2953.0 10 4131.0 11 5309.0 12 5309.0 13 5309.0 14 7901.0 15 10493.0 16 10493.0 17 10493.0 18 17917.0 19 25341.0 20 25341.0 21 25341.0 22 38209.0 23 51077.0 24 51077.0 25 51077.0 26 71454.5 27 91832.0 28 91832.0 29 91832.0 30 113742.0 31 135652.0 32 135652.0 33 135652.0 34 148665.5 35 161679.0 36 161679.0 37 161679.0 38 169172.5 39 176666.0 40 176666.0 41 176666.0 42 181954.5 43 187243.0 44 187243.0 45 187243.0 46 184859.5 47 182476.0 48 182476.0 49 182476.0 50 172189.5 51 161903.0 52 161903.0 53 161903.0 54 141120.0 55 120337.0 56 120337.0 57 120337.0 58 100116.5 59 79896.0 60 79896.0 61 79896.0 62 63858.5 63 47821.0 64 47821.0 65 47821.0 66 36224.0 67 24627.0 68 24627.0 69 24627.0 70 17579.0 71 10531.0 72 10531.0 73 10531.0 74 7310.0 75 4089.0 76 4089.0 77 4089.0 78 2737.5 79 1386.0 80 1386.0 81 1386.0 82 900.0 83 414.0 84 414.0 85 414.0 86 260.0 87 106.0 88 106.0 89 106.0 90 67.0 91 28.0 92 28.0 93 28.0 94 15.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3658480692550125 2 0.0 3 0.0 4 0.0 5 0.04758451306080303 6 0.0 7 0.0 8 0.0031678075267106837 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005661613451993562 25 9.436022419989271E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1483676.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.76724869545167 #Duplication Level Percentage of deduplicated Percentage of total 1 87.60366354107728 67.25092225690553 2 8.811563411499813 13.528789596126975 3 2.0095951488318797 4.628132717026504 4 0.6605264177160884 2.02827183114907 5 0.29575793227453373 1.1352261370285843 6 0.16585866112941453 0.7639507852329845 7 0.09592918729841364 0.5154953844942919 8 0.06041371073784585 0.3710235485461833 9 0.04750790592026865 0.3282346105903246 >10 0.21539483696660497 2.8756173292280898 >50 0.015944237899026846 0.8642755122067334 >100 0.014346146748078038 2.1770564761925204 >500 0.0022742602355495874 1.1409371616924973 >1k 0.0010496585702536557 1.6040672661120814 >5k 1.7494309504227594E-4 0.7879993874675685 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6654 0.44848066559006144 No Hit TATCAACGCAGAGTACTTTTTTTTT 5081 0.3424602136854677 No Hit GGTATCAACGCAGAGTACTTTTTTT 4675 0.31509574866749884 No Hit CTGTAGGACGTGGAATATGGCAAGA 2496 0.16823079971638014 No Hit CTTTAGGACGTGAAATATGGCGAGG 2305 0.15535736912910905 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2226 0.15003275647782938 No Hit GTCCTACAGTGGACATTTCTAAATT 2033 0.13702452557027275 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2029 0.13675492492970165 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1858 0.12522949754528617 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1527 0.10292004453802582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 6.74001601427805E-5 0.0 12 0.0 0.0 0.0 6.74001601427805E-5 0.0 13 0.0 0.0 0.0 6.74001601427805E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATCG 25 0.0060225595 19.005127 5 ACACCGA 60 4.1023153E-4 12.662826 6 GGTATCA 1340 0.0 12.306829 1 AATCCCG 90 7.479428E-6 11.608372 19 GGATAAG 95 1.30950575E-5 11.037582 1 TGCCGGT 80 3.782217E-4 10.683899 10 CACCTTT 550 0.0 10.187475 14 AGAACCG 75 0.0026415316 10.136067 5 CCACCTT 555 0.0 9.9245825 13 CTTATAC 115 1.022753E-5 9.915718 3 TAGGACC 415 0.0 9.846029 4 TGTAGGA 1565 0.0 9.775799 2 CTAGGAC 185 6.4392225E-10 9.759389 3 ACCTTTT 590 0.0 9.657761 15 GGCGAGG 695 0.0 9.566088 19 GTATCAA 2575 0.0 9.5139475 1 CTAGACC 80 0.004505207 9.502563 3 TACACTA 160 6.601658E-8 9.502563 5 CCTTATA 140 1.0539861E-6 9.5025625 2 CAAGTCG 130 4.25267E-6 9.497119 6 >>END_MODULE