FastQCFastQC Report
Thu 26 May 2016
SRR1512063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499437
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24610.49275484195203795No Hit
TATCAACGCAGAGTACTTTTTTTTT15080.3019399844224597No Hit
GGTATCAACGCAGAGTACTTTTTTT14970.29973750442998814No Hit
GTCCTACAGTGGACATTTCTAAATT9930.19882387568402018No Hit
GTCCTAAAGTGTGTATTTCTCATTT9740.1950195920606603No Hit
CTGTAGGACGTGGAATATGGCAAGA9640.19301733752204983No Hit
CTTTAGGACGTGAAATATGGCGAGG7930.1587787849118107No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6650.1331499268175966No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6420.12854474137879252No Hit
GTCCTACAGTGTGCATTTCTCATTT6150.12313865412454425No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5400.10812174508496566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5250.10511836327704996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGC250.006167766618.92390819
CTAGGAC500.001543017613.2467363
GGTATCA4350.013.1149051
TAGGACT655.6466582E-513.1011674
TAGGACC1600.013.0101874
GGACTGC751.5358988E-512.6159396
AAAATAG550.003156884812.0424885
TAAGACT550.003156884812.0424884
CCACCTT1353.929017E-1011.91505313
TTATACA1302.763045E-911.6454824
TTAAGCT658.280781E-411.6454824
GATATAC959.20891E-611.3892591
ACTGTTC1002.0157458E-611.3543458
AGGACCT3350.011.2978565
TCCTAGA700.001112987511.2413462
AGGACTG1354.993126E-911.2141685
TTTATAC855.5272016E-511.1317113
AGCTTTG600.00604403111.0389467
ACCAGTG600.00604403111.0389467
ATAACTC600.00604403111.0389465