FastQCFastQC Report
Thu 26 May 2016
SRR1512063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499437
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39790.7966970809131082No Hit
GGTATCAACGCAGAGTACTTTTTTT31880.6383187469090196No Hit
TATCAACGCAGAGTACTTTTTTTTT30150.6036797433910583No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14220.2847205953904096No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10030.20082613022263068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9490.19001395571413413No Hit
CTGTAGGACGTGGAATATGGCAAGA7870.15757743218864442No Hit
CTTTAGGACGTGAAATATGGCGAGG6560.1313478977328472No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT6370.12754361410948728No Hit
GTCCTAAAGTGTGTATTTCTCATTT6340.12694293774790413No Hit
GTCCTACAGTGGACATTTCTAAATT5980.11973482140890644No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5320.1065199414540773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.014.9141031
TTTATAC551.9500198E-413.8231373
AAAGGCG551.9500198E-413.8231375
TAGGACC1600.013.6611474
GGCGTGC500.001500933313.29544458
ACTGTGC957.3803676E-812.9955478
ACACCTC550.003071304912.0867686
GTGGTAT1751.8189894E-1211.4384691
CAGCACC600.005850560511.0873074
GATATAC951.3052788E-511.0371191
TATAGTG951.3495914E-511.0039435
TTAGGGT700.001483886710.8610364
GCTGTGC700.001492593810.8534248
TGTAGGA5900.010.7920042
AATGTCC1803.45608E-1110.5519418
TGTCCAC1803.45608E-1110.55194110
TAGAAAT2001.8189894E-1210.4537474
ACCTTTT1951.4551915E-1110.22726415
TGGTATC1606.0790626E-910.097372
TTCCACC1701.551598E-910.05537911