##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 499437 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.738930035219656 31.0 28.0 31.0 16.0 34.0 2 27.91711266886514 31.0 28.0 31.0 16.0 34.0 3 27.859986344624048 31.0 27.0 31.0 16.0 34.0 4 31.29248533849114 35.0 31.0 37.0 19.0 37.0 5 30.657982888732715 35.0 30.0 37.0 16.0 37.0 6 30.677248581903225 35.0 31.0 37.0 11.0 37.0 7 30.37816981921644 35.0 30.0 37.0 10.0 37.0 8 30.188239958192923 35.0 30.0 37.0 10.0 37.0 9 31.176178376852334 35.0 30.0 39.0 10.0 39.0 10 30.80691058131456 35.0 30.0 39.0 8.0 39.0 11 30.887829696237965 35.0 30.0 39.0 2.0 39.0 12 30.542254578655566 35.0 30.0 39.0 2.0 39.0 13 30.52842901106646 35.0 30.0 39.0 2.0 39.0 14 30.90815257980486 36.0 29.0 39.0 2.0 41.0 15 30.806730378406087 36.0 29.0 39.0 2.0 41.0 16 30.602186061505254 36.0 28.0 39.0 2.0 40.0 17 30.528521114775238 36.0 28.0 39.0 2.0 40.0 18 30.389628721940905 36.0 27.0 39.0 2.0 40.0 19 30.418278982133884 36.0 27.0 39.0 2.0 40.0 20 30.262073094304185 36.0 27.0 39.0 2.0 40.0 21 30.05022255059197 36.0 27.0 39.0 2.0 40.0 22 29.844108466132866 36.0 27.0 39.0 2.0 40.0 23 29.77250383932308 36.0 27.0 39.0 2.0 40.0 24 29.48772317629651 36.0 27.0 39.0 2.0 40.0 25 28.912992029024682 36.0 26.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5641105222145875 1101 2 0.28604736621628746 1101 3 0.7152276985563013 1101 4 0.23979805218431594 1101 5 0.6738350813671694 1101 6 0.08750301806093574 1101 7 0.2606040792973623 1101 8 0.18673784669328697 1101 9 -0.04357005027962302 1101 10 0.6544704967270683 1101 11 0.7244135094663484 1101 12 0.85086419313728 1101 13 0.7265909271669386 1101 14 0.8027721127423533 1101 15 0.9980709141852735 1101 16 0.5607002525837856 1101 17 0.5222933208536986 1101 18 0.6196552358405256 1101 19 0.18082968120916476 1101 20 -0.2688239277737523 1101 21 0.19325877727367313 1101 22 0.2546913154081274 1101 23 0.058110619460936874 1101 24 -0.28791473423788716 1101 25 -0.2763208170064466 1102 1 -0.10680501171464485 1102 2 -0.204062117043879 1102 3 0.13306236296631013 1102 4 0.14271760578879977 1102 5 -0.08464061546469281 1102 6 -0.13569473938339627 1102 7 0.11999478115381734 1102 8 -0.06787684627757073 1102 9 0.5334384650850588 1102 10 0.17679027247150003 1102 11 0.14071435042472302 1102 12 0.2582001004731893 1102 13 0.255336894269071 1102 14 0.5787281078764295 1102 15 0.32432574044009854 1102 16 0.18821916092461066 1102 17 0.2977997470450582 1102 18 -0.12977619167309484 1102 19 -0.19612065464528072 1102 20 0.049867562150613054 1102 21 0.685563214783592 1102 22 0.5554529380875586 1102 23 0.4896703592398559 1102 24 0.478362306011757 1102 25 0.30848904177601355 1103 1 -0.18902982412813785 1103 2 0.17023671008513475 1103 3 0.5649120023631191 1103 4 0.5927722426358883 1103 5 0.4547197691787126 1103 6 -0.07010138322400294 1103 7 0.35794189268583665 1103 8 0.714792345825586 1103 9 0.26341588185492526 1103 10 0.37594522256984675 1103 11 0.3959048508982441 1103 12 0.9840178319512987 1103 13 0.636002735312271 1103 14 0.457478016815017 1103 15 0.5517097282014447 1103 16 0.8534337016108502 1103 17 0.7787715544319589 1103 18 0.7557996806952332 1103 19 0.7811139924006874 1103 20 0.8216239546617246 1103 21 0.8353774495034862 1103 22 0.04797861740568976 1103 23 0.1603777242868425 1103 24 0.7669905569041582 1103 25 0.15189983801699825 1104 1 -0.057388643149341334 1104 2 0.056354005074368274 1104 3 -0.30034838959338117 1104 4 -0.17571065944797581 1104 5 -0.34477295006511 1104 6 -0.07488952964201445 1104 7 -0.4633566000699041 1104 8 -0.2980210551451812 1104 9 -0.2700573490490541 1104 10 -0.29928195891988096 1104 11 -0.3469147542256579 1104 12 -0.13504351617468302 1104 13 0.047768729254098474 1104 14 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0.4089930752251867 1107 12 0.8418297493202296 1107 13 0.6530584441645324 1107 14 0.03576871709095286 1107 15 0.41817255167821443 1107 16 -0.004594488410777586 1107 17 0.3394695930627307 1107 18 0.09481825031265245 1107 19 0.17950313402198503 1107 20 0.3778697597180276 1107 21 -0.06621849351985531 1107 22 0.04154264156856868 1107 23 0.3978227239139578 1107 24 0.640471055077029 1107 25 0.4230982868200428 1108 1 0.01225527466847609 1108 2 -0.16400040178531583 1108 3 -0.313263603044696 1108 4 -0.27514485734925387 1108 5 -0.15134173728392497 1108 6 -0.28261809693667317 1108 7 -0.20466396082775873 1108 8 0.09187598867211477 1108 9 -0.2442649976577762 1108 10 -0.5068582241691217 1108 11 0.1583472814434792 1108 12 0.05924439561197303 1108 13 -0.022472974091979125 1108 14 -0.19183857804458881 1108 15 -0.03904944576973435 1108 16 -0.2956451871129637 1108 17 0.009881344953427629 1108 18 -0.028652852360778525 1108 19 -0.2317741371491593 1108 20 -0.39768966887118395 1108 21 -0.02638157590135748 1108 22 0.07966781663314748 1108 23 -0.7030378157517845 1108 24 -0.6669345656630661 1108 25 -0.5032034155857339 1109 1 -0.3329331726254168 1109 2 -0.5684462640367229 1109 3 -0.7006931881805478 1109 4 -0.18441472134352566 1109 5 -0.7108481329765581 1109 6 -0.63720556698118 1109 7 -0.5897126676840649 1109 8 -0.49438805539747577 1109 9 -0.9426248370651997 1109 10 -0.7116526594496904 1109 11 -1.1127460552095947 1109 12 -0.8123204483082311 1109 13 -0.9754000538591825 1109 14 -0.7330059864663596 1109 15 -0.5581120333020237 1109 16 -0.577717018055047 1109 17 -0.6368149919175075 1109 18 -0.7786995362486131 1109 19 -0.6505363916697142 1109 20 -0.5964385406772301 1109 21 -0.6689852919388279 1109 22 -0.5315798880757008 1109 23 -0.5863669994061986 1109 24 0.02387026456319319 1109 25 -0.11840368946454305 1110 1 -0.397193544229161 1110 2 -0.43368544115539365 1110 3 -0.3939722644620183 1110 4 -0.37750706207366136 1110 5 -0.6474047294099066 1110 6 -0.6408858134721065 1110 7 -0.1908844332687032 1110 8 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2315 10 -0.5837339395945627 2315 11 -0.40261563783918675 2315 12 -0.6595196961012348 2315 13 -0.9032874317561372 2315 14 -0.5452067142962775 2315 15 -1.0223822009815002 2315 16 -0.4601063199999871 2315 17 -1.204139259299236 2315 18 -1.150101740633005 2315 19 -0.947919338162027 2315 20 -0.8895554984178204 2315 21 -1.0709894360421863 2315 22 -0.7812708215436892 2315 23 -0.4476265978808911 2315 24 -0.6343892120261039 2315 25 -0.25621253834406943 2316 1 -0.8029842266438614 2316 2 -1.0287805855123509 2316 3 -1.1914952303412853 2316 4 -1.6652859334647374 2316 5 -1.6128902937143685 2316 6 -1.221988175676831 2316 7 -1.2123411261820891 2316 8 -1.7749875283882517 2316 9 -1.6196422942457005 2316 10 -1.5813660981848692 2316 11 -1.6967052119948391 2316 12 -1.793183425910339 2316 13 -1.6027551263229505 2316 14 -1.9181509140025916 2316 15 -1.447464800100441 2316 16 -1.439805292099841 2316 17 -1.6009454267001182 2316 18 -1.3980918287387318 2316 19 -1.2724237434483712 2316 20 -1.62672510194205 2316 21 -1.305974751754377 2316 22 -1.962162892028271 2316 23 -1.9790876845181877 2316 24 -1.925973279573828 2316 25 -1.5554507909138202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31365.0 3 1.0 4 477.0 5 3404.0 6 4194.0 7 3340.0 8 2741.0 9 2459.0 10 2286.0 11 2108.0 12 2029.0 13 2004.0 14 1882.0 15 1878.0 16 1960.0 17 2040.0 18 2170.0 19 2478.0 20 3054.0 21 3817.0 22 4870.0 23 5872.0 24 6910.0 25 7943.0 26 9088.0 27 10170.0 28 11963.0 29 14089.0 30 17100.0 31 21078.0 32 25885.0 33 31775.0 34 38624.0 35 47592.0 36 57889.0 37 66009.0 38 46893.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.751783622617396 24.412574865939487 16.034420752105085 18.801220759338026 2 18.18868045419142 25.855913758892513 38.30453090179542 17.650874885120647 3 21.584504151674786 28.81564641786652 31.359110358263404 18.240739072195293 4 16.911041833104075 21.94511019407853 37.08375630960462 24.060091663212777 5 12.798729161575167 40.95248779041582 35.396175465446575 10.852607582562431 6 29.423330670334796 41.22521959726652 17.711342972186685 11.640106760212 7 26.2199236340119 34.95455883324624 23.227954676966263 15.597562855775603 8 24.747655766623883 39.41455737389796 20.776625545385922 15.061161314092232 9 27.1914175361457 16.29855216974313 21.463968428450436 35.04606186566073 10 15.82822257862353 31.66685688084783 33.985868087466486 18.519052453062148 11 33.63106858322471 23.910323023724715 25.55417399992391 16.90443439312666 12 23.32246109118868 27.623704291031704 32.035071490498304 17.018763127281318 13 29.860823287021187 23.795994289570054 26.267377066576962 20.07580535683179 14 21.243720429203282 24.743461137240534 28.8234552105671 25.189363222989087 15 23.12203541187377 33.33313310787947 24.421498607431968 19.12333287281479 16 21.272352669105413 30.545193888318245 28.82966219963679 19.35279124293955 17 21.0863432224685 30.80208314562197 28.77199726892481 19.33957636298472 18 20.768184976283294 28.713931887305105 31.85506880747722 18.66281432893438 19 23.060566197538428 28.060195780448787 30.265078478366643 18.614159543646146 20 23.021722459489386 28.648257938438682 30.41184373604679 17.918175866025145 21 22.511147552143715 27.96408756259548 29.879444254230265 19.64532063103054 22 22.839717521929693 29.14401616219864 30.12532111157163 17.890945204300042 23 21.75209285655648 29.102169042341675 30.432667183248334 18.713070917853504 24 22.457969900120943 28.28731848363249 30.958302296337237 18.296409319909333 25 22.125199729289225 28.796458334167625 30.664602964202903 18.413738972340248 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1170.0 1 1170.0 2 865.0 3 560.0 4 560.0 5 560.0 6 1093.5 7 1627.0 8 1627.0 9 1627.0 10 2188.0 11 2749.0 12 2749.0 13 2749.0 14 3699.5 15 4650.0 16 4650.0 17 4650.0 18 7528.0 19 10406.0 20 10406.0 21 10406.0 22 15610.0 23 20814.0 24 20814.0 25 20814.0 26 28554.0 27 36294.0 28 36294.0 29 36294.0 30 43808.5 31 51323.0 32 51323.0 33 51323.0 34 54888.5 35 58454.0 36 58454.0 37 58454.0 38 59048.5 39 59643.0 40 59643.0 41 59643.0 42 59940.0 43 60237.0 44 60237.0 45 60237.0 46 58424.0 47 56611.0 48 56611.0 49 56611.0 50 52763.5 51 48916.0 52 48916.0 53 48916.0 54 42277.5 55 35639.0 56 35639.0 57 35639.0 58 29620.5 59 23602.0 60 23602.0 61 23602.0 62 18747.5 63 13893.0 64 13893.0 65 13893.0 66 10654.0 67 7415.0 68 7415.0 69 7415.0 70 5420.0 71 3425.0 72 3425.0 73 3425.0 74 2382.0 75 1339.0 76 1339.0 77 1339.0 78 903.0 79 467.0 80 467.0 81 467.0 82 297.0 83 127.0 84 127.0 85 127.0 86 93.5 87 60.0 88 60.0 89 60.0 90 35.0 91 10.0 92 10.0 93 10.0 94 6.5 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3431864279178355 2 0.0 3 0.0 4 0.0 5 0.04785388347279036 6 0.0 7 0.0 8 0.004004509077220951 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.006607439977414569 25 0.002202479992471523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 499437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.56698424026563 #Duplication Level Percentage of deduplicated Percentage of total 1 90.9912763129345 72.39901448392915 2 6.455924832748705 10.273569388473115 3 1.3347989768910526 3.1861778747463925 4 0.4720999287273397 1.5025427035551504 5 0.20722648103054445 0.8244193075161518 6 0.12247907226630257 0.5847174247665151 7 0.07947096615218893 0.44262855779729304 8 0.05542971344265223 0.35283001087471605 9 0.0425538143359762 0.30472908071704274 >10 0.19574543134588301 2.810360331070612 >50 0.024388679319987814 1.31556170514166 >100 0.015588635539222215 2.522492922056069 >500 0.0017600072382989943 0.9588633972094317 >1k 0.0012571480273564245 2.522092812146736 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3979 0.7966970809131082 No Hit GGTATCAACGCAGAGTACTTTTTTT 3188 0.6383187469090196 No Hit TATCAACGCAGAGTACTTTTTTTTT 3015 0.6036797433910583 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1422 0.2847205953904096 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1003 0.20082613022263068 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 949 0.19001395571413413 No Hit CTGTAGGACGTGGAATATGGCAAGA 787 0.15757743218864442 No Hit CTTTAGGACGTGAAATATGGCGAGG 656 0.1313478977328472 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 637 0.12754361410948728 No Hit GTCCTAAAGTGTGTATTTCTCATTT 634 0.12694293774790413 No Hit GTCCTACAGTGGACATTTCTAAATT 598 0.11973482140890644 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 532 0.1065199414540773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.0022545386104754E-4 0.0 5 0.0 0.0 0.0 2.0022545386104754E-4 0.0 6 0.0 0.0 0.0 2.0022545386104754E-4 0.0 7 0.0 0.0 0.0 2.0022545386104754E-4 0.0 8 0.0 0.0 0.0 2.0022545386104754E-4 0.0 9 0.0 0.0 0.0 2.0022545386104754E-4 0.0 10 0.0 2.0022545386104754E-4 0.0 2.0022545386104754E-4 0.0 11 0.0 2.0022545386104754E-4 0.0 2.0022545386104754E-4 0.0 12 0.0 2.0022545386104754E-4 0.0 2.0022545386104754E-4 0.0 13 0.0 2.0022545386104754E-4 0.0 2.0022545386104754E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 735 0.0 14.914103 1 TTTATAC 55 1.9500198E-4 13.823137 3 AAAGGCG 55 1.9500198E-4 13.823137 5 TAGGACC 160 0.0 13.661147 4 GGCGTGC 50 0.0015009333 13.2954445 8 ACTGTGC 95 7.3803676E-8 12.995547 8 ACACCTC 55 0.0030713049 12.086768 6 GTGGTAT 175 1.8189894E-12 11.438469 1 CAGCACC 60 0.0058505605 11.087307 4 GATATAC 95 1.3052788E-5 11.037119 1 TATAGTG 95 1.3495914E-5 11.003943 5 TTAGGGT 70 0.0014838867 10.861036 4 GCTGTGC 70 0.0014925938 10.853424 8 TGTAGGA 590 0.0 10.792004 2 AATGTCC 180 3.45608E-11 10.551941 8 TGTCCAC 180 3.45608E-11 10.551941 10 TAGAAAT 200 1.8189894E-12 10.453747 4 ACCTTTT 195 1.4551915E-11 10.227264 15 TGGTATC 160 6.0790626E-9 10.09737 2 TTCCACC 170 1.551598E-9 10.055379 11 >>END_MODULE