FastQCFastQC Report
Thu 26 May 2016
SRR1512062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628580
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29630.4713799357281492No Hit
GGTATCAACGCAGAGTACTTTTTTT19580.31149575233065No Hit
TATCAACGCAGAGTACTTTTTTTTT17950.2855642877597124No Hit
GTCCTACAGTGGACATTTCTAAATT11880.18899742276241688No Hit
GTCCTAAAGTGTGTATTTCTCATTT10470.1665659104648573No Hit
CTGTAGGACGTGGAATATGGCAAGA10200.16227051449298419No Hit
CTTTAGGACGTGAAATATGGCGAGG8760.1393617359763276No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8560.13617996118234751No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8010.12743008049890228No Hit
GTCCTACAGTGTGCATTTCTCATTT7780.1237710394858252No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7070.11247573896719591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.014.8708194
TTAGACC400.00541184614.1948733
AGGACTG1001.0588337E-813.24854855
GCGCCAC500.001542202413.248548513
GTGCGCC500.001542202413.248548511
CTATTAG450.00920901113.0990721
CTCTTAG707.9560836E-512.63124751
GTCCTAA2850.012.4096481
TCCAACG701.12902264E-412.16703318
GTGTAGG1051.7515958E-712.16342451
ATAGGAC951.0861586E-611.9535773
GTCCTAT751.5148535E-411.7891661
GGTATCA4600.011.7464511
TTAGACT658.276506E-411.6470764
CACTGTT1407.203198E-1011.4910877
TCCTAAA3700.011.4174352
AGGACCT5100.011.3187885
TGTAGGA6950.011.3085522
GTAGGAC7200.011.3033263
GTGTAGC1155.6956196E-711.1057361
Produced by FastQC (version 0.11.5)