##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512062_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628580 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.70054885615196 31.0 30.0 34.0 25.0 34.0 2 27.3046517547488 31.0 27.0 34.0 10.0 34.0 3 28.922751280664354 31.0 28.0 34.0 16.0 34.0 4 33.18500270450858 35.0 33.0 37.0 28.0 37.0 5 33.570304495847786 37.0 35.0 37.0 28.0 37.0 6 33.83450316586592 37.0 35.0 37.0 30.0 37.0 7 33.912937096312326 37.0 35.0 37.0 30.0 37.0 8 33.954226987813804 37.0 35.0 37.0 30.0 37.0 9 35.59485984282033 39.0 37.0 39.0 30.0 39.0 10 35.47560851442935 39.0 37.0 39.0 30.0 39.0 11 35.497548442521236 39.0 37.0 39.0 30.0 39.0 12 35.413212319832 39.0 37.0 39.0 30.0 39.0 13 35.40293359636005 39.0 37.0 39.0 30.0 39.0 14 36.59062012790735 40.0 37.0 41.0 30.0 41.0 15 36.54218556110599 40.0 37.0 41.0 30.0 41.0 16 36.520508129434596 40.0 37.0 41.0 30.0 41.0 17 36.48206115371154 40.0 37.0 41.0 30.0 41.0 18 36.44321327436444 40.0 37.0 41.0 30.0 41.0 19 36.46915746603455 40.0 37.0 41.0 30.0 41.0 20 36.4246826179643 40.0 37.0 41.0 30.0 41.0 21 36.36365458652836 40.0 37.0 41.0 30.0 41.0 22 36.27763371408572 40.0 37.0 41.0 30.0 41.0 23 36.18565815011613 40.0 37.0 41.0 29.0 41.0 24 36.10366381367527 40.0 37.0 41.0 29.0 41.0 25 36.00276973495816 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6927270547175368 1101 2 3.165532766909454 1101 3 1.6830670066074234 1101 4 0.9310400286603198 1101 5 0.44400256878184763 1101 6 0.22427527812466508 1101 7 0.1242355814859124 1101 8 -0.12302384824185708 1101 9 -0.08090630747096128 1101 10 -0.1187452847156294 1101 11 -0.061435385079626315 1101 12 -0.3189899885012366 1101 13 -0.4302681003521016 1101 14 -0.39892599978080057 1101 15 -0.11945809785768091 1101 16 -0.3354780928683567 1101 17 -0.5354364295304066 1101 18 -0.28821138595187534 1101 19 -0.41877787603314687 1101 20 -0.47627197283078715 1101 21 -0.4048777532603083 1101 22 -0.24083723189291106 1101 23 -0.4552333641543882 1101 24 -0.4339379689488041 1101 25 -0.3489512799737895 1102 1 1.098763063186226 1102 2 3.5255802378810372 1102 3 2.2447275808903946 1102 4 1.2241484675765975 1102 5 1.0012695971321008 1102 6 0.9726005617926603 1102 7 0.7941345195917009 1102 8 0.583230907125504 1102 9 0.6951262275119987 1102 10 0.4807866217318235 1102 11 0.8643643436576767 1102 12 0.5744091480894582 1102 13 0.3715529985926196 1102 14 0.696791275021539 1102 15 0.49151977107612765 1102 16 0.774308866673806 1102 17 0.613272178098029 1102 18 0.738478630257724 1102 19 0.7539039857552012 1102 20 0.7082466185880492 1102 21 0.7087300405800434 1102 22 0.6723793812931049 1102 23 0.5402262937899707 1102 24 0.594021523591465 1102 25 0.7099925322594984 1103 1 1.1922357262684287 1103 2 3.138077808652561 1103 3 1.9457097989379442 1103 4 1.4900113295316402 1103 5 1.1467884803347985 1103 6 1.0629273739971055 1103 7 0.9865051226881576 1103 8 0.9286429042752076 1103 9 1.1267393971693167 1103 10 0.8388063146773419 1103 11 1.0416980224812846 1103 12 0.8555801981639561 1103 13 1.0298253312997474 1103 14 0.9369326561188984 1103 15 1.0123625514090904 1103 16 1.0495728428349764 1103 17 1.057444458981621 1103 18 0.90905500520104 1103 19 1.010462902643198 1103 20 1.1714316610185733 1103 21 1.1770690960960053 1103 22 1.1710109159177051 1103 23 1.2677802226621608 1103 24 1.200830651012602 1103 25 1.2213086882483566 1104 1 1.3854481158596492 1104 2 4.071868252192306 1104 3 2.53837778297083 1104 4 1.6817198582875221 1104 5 1.4281810792761434 1104 6 0.9876986204953724 1104 7 0.6184322072614847 1104 8 0.7725984947489053 1104 9 0.8327603956694247 1104 10 0.9086505321538709 1104 11 0.9689492505681656 1104 12 0.9402368496729707 1104 13 0.7888822240613749 1104 14 0.8775083292851065 1104 15 0.7997586897801625 1104 16 0.8226922231966967 1104 17 0.819009109125659 1104 18 0.939743289371215 1104 19 0.7719918410523618 1104 20 0.9621847024679937 1104 21 1.0677872194046643 1104 22 0.8929037965849602 1104 23 1.1154101538575532 1104 24 1.2890664119503086 1104 25 1.0933614781284433 1105 1 0.6782997087112435 1105 2 3.602870583194637 1105 3 1.8155080601011093 1105 4 1.3280420546097176 1105 5 0.9612942623893304 1105 6 0.5162404490372055 1105 7 0.24668913551840888 1105 8 0.22281864496905257 1105 9 0.09779536070438866 1105 10 0.08423975774309866 1105 11 0.22521705683597304 1105 12 0.12801203744815837 1105 13 0.07165870683785869 1105 14 -0.15582500404823207 1105 15 0.234296374317843 1105 16 0.1153236658281358 1105 17 0.09228028239682828 1105 18 0.040636840264767216 1105 19 0.12148679054730849 1105 20 0.2286571189404114 1105 21 0.20552355714099946 1105 22 0.3820516857328471 1105 23 0.4211988096462065 1105 24 0.5760775489614502 1105 25 0.4687046534716188 1106 1 0.41464500738634413 1106 2 2.9785364705095425 1106 3 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1107 14 -0.35940198694091663 1107 15 -0.3412137816744263 1107 16 -0.2046325055277194 1107 17 -0.18979710108023795 1107 18 -0.349718501645782 1107 19 -0.1151690367772602 1107 20 -0.12977871686711495 1107 21 -0.10837907729040808 1107 22 -0.013508896763973155 1107 23 0.04321916875831988 1107 24 -0.09965410663023988 1107 25 0.18481757113974595 1108 1 0.36248049967169393 1108 2 2.081350295572655 1108 3 1.1039004586629986 1108 4 0.4792233591808497 1108 5 0.38119667635953647 1108 6 0.20093485068075978 1108 7 0.42316576623233004 1108 8 0.36888952324332536 1108 9 0.4187506765749589 1108 10 0.46449142697781554 1108 11 0.26648567522323674 1108 12 0.5743397210639785 1108 13 0.4966201860366297 1108 14 0.39471428716903034 1108 15 0.4039163024123482 1108 16 0.3562327567372279 1108 17 0.3464148483958027 1108 18 0.3424601581897804 1108 19 0.3927759533618982 1108 20 0.4590371908459119 1108 21 0.6240649028009884 1108 22 0.586366015470908 1108 23 0.530829009954374 1108 24 0.3652146830138321 1108 25 0.3046574012887717 1109 1 0.6856471557847179 1109 2 3.006302159228955 1109 3 1.6049781029958083 1109 4 0.9431451402607465 1109 5 0.634402052620402 1109 6 0.41524418502724103 1109 7 0.4447796294993154 1109 8 0.137914812120016 1109 9 0.17499198447635678 1109 10 0.20951303849582814 1109 11 0.13518661543567134 1109 12 0.11625058733054772 1109 13 0.14325134555378582 1109 14 0.09212020143122146 1109 15 -0.05652277609034684 1109 16 -0.09097908773488683 1109 17 0.24957929996982386 1109 18 0.08182267117662434 1109 19 0.05966307392907311 1109 20 0.2355133525089741 1109 21 0.2668076307538385 1109 22 0.4939653428519861 1109 23 0.27873997472161705 1109 24 0.4005482837061578 1109 25 0.4168989249535642 1110 1 0.03506245815691855 1110 2 1.4253391715168853 1110 3 0.7670971214462661 1110 4 0.22046629703395837 1110 5 0.2840215351166009 1110 6 0.006779990796253799 1110 7 -0.08309652597456818 1110 8 -0.040152530197239855 1110 9 -0.10582622096597305 1110 10 -0.24329165393465502 1110 11 -0.2418561138957358 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-2.130878681544253 2315 18 -2.1541066843670222 2315 19 -2.0486164767626605 2315 20 -1.954422278384797 2315 21 -1.95870532384766 2315 22 -2.285326001756573 2315 23 -2.0860891537870074 2315 24 -2.042603676703017 2315 25 -2.077833352284266 2316 1 -1.5993235386881253 2316 2 -1.5539928270269812 2316 3 -1.232038299012416 2316 4 -1.4976929069461384 2316 5 -1.4959535892615108 2316 6 -1.6074931556665533 2316 7 -1.5611240711753922 2316 8 -1.7790131053701614 2316 9 -2.0495587776945214 2316 10 -2.272250518150166 2316 11 -2.247792893412786 2316 12 -2.3764542673912814 2316 13 -2.0816316595122046 2316 14 -2.827093660764646 2316 15 -2.9469564524573713 2316 16 -2.4400698212817673 2316 17 -2.6509806854909996 2316 18 -2.9448561493327006 2316 19 -2.910014792446809 2316 20 -2.4631565400374242 2316 21 -2.5776288625785853 2316 22 -2.4572631039550785 2316 23 -2.6029531577893437 2316 24 -2.5408491810249814 2316 25 -2.886089557292742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22357.0 3 0.0 4 196.0 5 838.0 6 999.0 7 916.0 8 812.0 9 800.0 10 727.0 11 688.0 12 688.0 13 670.0 14 689.0 15 694.0 16 727.0 17 803.0 18 872.0 19 1153.0 20 1436.0 21 1761.0 22 2083.0 23 2463.0 24 2976.0 25 3349.0 26 3857.0 27 4349.0 28 5222.0 29 6349.0 30 7937.0 31 10762.0 32 14708.0 33 20759.0 34 30035.0 35 44510.0 36 71739.0 37 107741.0 38 251915.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86181552069744 19.72525374653982 12.919278373476725 25.493652359286013 2 15.985828839467839 21.724818524911015 40.1848196810663 22.10453295455484 3 18.74272169015877 24.765662286423368 30.319132011836203 26.17248401158166 4 11.955836965859557 17.34067262719145 38.90403767221356 31.799452734735432 5 12.2043335772694 38.25002386331096 36.459639186738364 13.086003372681281 6 29.62264150943396 38.55531515479335 18.62945050749308 13.192592828279615 7 26.18648382067517 32.486557001495434 23.371408571701295 17.9555506061281 8 23.50551086901186 37.64445349488367 21.52802525072545 17.322010385379016 9 25.33869992681918 15.701740430812308 21.4061853701995 37.553374272169016 10 14.39148557065131 28.97276400776353 35.423016958859655 21.21273346272551 11 32.31490025136021 23.084412485284293 25.95723694676891 18.643450316586595 12 22.45362563237774 25.134907251264753 32.84896115052977 19.56250596582774 13 27.83050685672468 22.703554042444875 26.645932100925897 22.820006999904546 14 19.830570492220563 22.997231855929236 28.790130134589077 28.382067517261127 15 21.46822997868211 31.981768430430495 24.575869419962455 21.974132170924943 16 20.391676477138947 28.872856279232558 29.341372617646122 21.394094625982373 17 20.037385853829264 29.36571319482007 29.31798657291037 21.278914378440295 18 19.94097807757167 27.56594228260524 31.932769098603202 20.56031054121989 19 21.734897284191803 27.298398451109566 29.553325834938803 21.413378429759824 20 21.7866012068491 27.785369858044888 30.1136372675511 20.31439166755491 21 20.985747217135795 27.23985370176223 29.319942282513416 22.454456798588563 22 21.33119729166037 28.694177483426852 29.449693514080217 20.524931710832565 23 20.28849243848814 28.380248751944865 30.015368021152504 21.315890788414485 24 21.269721069268936 27.169218188962724 30.791674554829225 20.769386186939112 25 20.858761016895226 27.791689204238125 30.3035413153457 21.046008463520952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 941.0 1 941.0 2 692.0 3 443.0 4 443.0 5 443.0 6 954.5 7 1466.0 8 1466.0 9 1466.0 10 2044.0 11 2622.0 12 2622.0 13 2622.0 14 3578.5 15 4535.0 16 4535.0 17 4535.0 18 7471.5 19 10408.0 20 10408.0 21 10408.0 22 15540.5 23 20673.0 24 20673.0 25 20673.0 26 28492.0 27 36311.0 28 36311.0 29 36311.0 30 45688.5 31 55066.0 32 55066.0 33 55066.0 34 60909.5 35 66753.0 36 66753.0 37 66753.0 38 70124.0 39 73495.0 40 73495.0 41 73495.0 42 75875.0 43 78255.0 44 78255.0 45 78255.0 46 77895.5 47 77536.0 48 77536.0 49 77536.0 50 73477.0 51 69418.0 52 69418.0 53 69418.0 54 61055.0 55 52692.0 56 52692.0 57 52692.0 58 44628.5 59 36565.0 60 36565.0 61 36565.0 62 29227.5 63 21890.0 64 21890.0 65 21890.0 66 16745.5 67 11601.0 68 11601.0 69 11601.0 70 8333.5 71 5066.0 72 5066.0 73 5066.0 74 3491.5 75 1917.0 76 1917.0 77 1917.0 78 1274.0 79 631.0 80 631.0 81 631.0 82 422.0 83 213.0 84 213.0 85 213.0 86 137.5 87 62.0 88 62.0 89 62.0 90 38.0 91 14.0 92 14.0 93 14.0 94 7.5 95 1.0 96 1.0 97 1.0 98 3.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.6789271055394703 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 6.363549587960164E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.590887396990041E-4 20 1.590887396990041E-4 21 1.590887396990041E-4 22 4.7726621909701235E-4 23 3.181774793980082E-4 24 1.590887396990041E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628580.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.93375458005548 #Duplication Level Percentage of deduplicated Percentage of total 1 89.83817933844297 72.70941158496555 2 7.668113969377781 12.412185081790328 3 1.4081754619356541 3.4190678172586946 4 0.43070623723779466 1.394346916028113 5 0.19515619985792304 0.78973619920387 6 0.10826775128738705 0.5257509366976723 7 0.06732121201386997 0.3813990915813704 8 0.04366518845334781 0.28271901167800995 9 0.03548152252059414 0.258448755222763 >10 0.17272248787823619 2.5960393356685887 >50 0.01908783999148498 1.1132559084386653 >100 0.01073682900270003 1.7767256601446548 >500 0.001192981000136058 0.736373210782206 >1k 0.001192981000136058 1.6045404905396365 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2963 0.4713799357281492 No Hit GGTATCAACGCAGAGTACTTTTTTT 1958 0.31149575233065 No Hit TATCAACGCAGAGTACTTTTTTTTT 1795 0.2855642877597124 No Hit GTCCTACAGTGGACATTTCTAAATT 1188 0.18899742276241688 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1047 0.1665659104648573 No Hit CTGTAGGACGTGGAATATGGCAAGA 1020 0.16227051449298419 No Hit CTTTAGGACGTGAAATATGGCGAGG 876 0.1393617359763276 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 856 0.13617996118234751 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 801 0.12743008049890228 No Hit GTCCTACAGTGTGCATTTCTCATTT 778 0.1237710394858252 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 707 0.11247573896719591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.590887396990041E-4 2 0.0 0.0 0.0 0.0 1.590887396990041E-4 3 0.0 0.0 0.0 0.0 1.590887396990041E-4 4 0.0 0.0 0.0 0.0 1.590887396990041E-4 5 0.0 0.0 0.0 0.0 1.590887396990041E-4 6 0.0 0.0 0.0 0.0 1.590887396990041E-4 7 0.0 0.0 0.0 0.0 1.590887396990041E-4 8 0.0 0.0 0.0 0.0 1.590887396990041E-4 9 0.0 0.0 0.0 0.0 4.772662190970123E-4 10 0.0 0.0 0.0 0.0 4.772662190970123E-4 11 0.0 0.0 0.0 0.0 4.772662190970123E-4 12 0.0 0.0 0.0 0.0 4.772662190970123E-4 13 0.0 0.0 0.0 0.0 4.772662190970123E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 280 0.0 14.870819 4 TTAGACC 40 0.005411846 14.194873 3 AGGACTG 100 1.0588337E-8 13.2485485 5 GCGCCAC 50 0.0015422024 13.2485485 13 GTGCGCC 50 0.0015422024 13.2485485 11 CTATTAG 45 0.009209011 13.099072 1 CTCTTAG 70 7.9560836E-5 12.6312475 1 GTCCTAA 285 0.0 12.409648 1 TCCAACG 70 1.12902264E-4 12.167033 18 GTGTAGG 105 1.7515958E-7 12.1634245 1 ATAGGAC 95 1.0861586E-6 11.953577 3 GTCCTAT 75 1.5148535E-4 11.789166 1 GGTATCA 460 0.0 11.746451 1 TTAGACT 65 8.276506E-4 11.647076 4 CACTGTT 140 7.203198E-10 11.491087 7 TCCTAAA 370 0.0 11.417435 2 AGGACCT 510 0.0 11.318788 5 TGTAGGA 695 0.0 11.308552 2 GTAGGAC 720 0.0 11.303326 3 GTGTAGC 115 5.6956196E-7 11.105736 1 >>END_MODULE