FastQCFastQC Report
Thu 26 May 2016
SRR1512062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628580
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43590.6934678163479588No Hit
GGTATCAACGCAGAGTACTTTTTTT33870.538833561360527No Hit
TATCAACGCAGAGTACTTTTTTTTT31000.4931750930669127No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15270.24292850552037926No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10550.16783862038244934No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10090.16052053835629512No Hit
CTGTAGGACGTGGAATATGGCAAGA8990.14302077698940469No Hit
GTCCTACAGTGGACATTTCTAAATT8840.14063444589391963No Hit
CTTTAGGACGTGAAATATGGCGAGG8100.12886187915619332No Hit
GTCCTAAAGTGTGTATTTCTCATTT7810.12424830570492221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAG250.00603867618.99427214
GGTATCA7250.014.4635251
TATACCT400.00527110314.2513725
TAACCTG551.9556304E-413.8195125
TAGGACC3150.013.5727374
GTGTAGA957.0351234E-813.0448531
TAGATTG701.0879282E-412.2154635
CCAGTAC701.0879282E-412.2154633
TCTAGAT550.003062739312.0920733
TAGACCT951.0352851E-612.0011574
CTGTAGG6650.011.75470351
TGTAGGA7550.011.4514342
ACAATAC752.0682185E-411.4010983
AACAGGT752.0758764E-411.3965637
GTAGGAC7350.011.3752453
AGTAGAG600.005864853511.0844015
CACCTTT1803.6379788E-1211.07999214
GTTCTAA1053.3151573E-610.8946031
AAGGCGT700.001493012510.853876
CATACTG803.7605915E-410.688535
Produced by FastQC (version 0.11.5)