##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628580 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30906487638805 31.0 28.0 31.0 22.0 34.0 2 28.493924401030895 31.0 28.0 31.0 22.0 34.0 3 28.474211715294793 31.0 28.0 33.0 22.0 34.0 4 31.95934964523211 35.0 32.0 37.0 24.0 37.0 5 31.37660122816507 35.0 32.0 37.0 18.0 37.0 6 31.37183492952369 35.0 32.0 37.0 17.0 37.0 7 31.070918578383022 35.0 32.0 37.0 16.0 37.0 8 30.912994368258616 35.0 31.0 37.0 11.0 37.0 9 31.990330586401093 37.0 32.0 39.0 11.0 39.0 10 31.641211938019026 37.0 30.0 39.0 10.0 39.0 11 31.70723694676891 37.0 31.0 39.0 10.0 39.0 12 31.370552674281715 37.0 30.0 39.0 10.0 39.0 13 31.355235610423495 37.0 30.0 39.0 10.0 39.0 14 31.862127334627257 37.0 31.0 40.0 8.0 41.0 15 31.767319991091032 37.0 30.0 40.0 2.0 41.0 16 31.58677177129403 37.0 30.0 40.0 2.0 41.0 17 31.516618409748958 37.0 30.0 40.0 2.0 41.0 18 31.383203410862578 37.0 30.0 40.0 2.0 41.0 19 31.399600687263355 37.0 30.0 40.0 2.0 41.0 20 31.22503738585383 37.0 30.0 40.0 2.0 41.0 21 31.04115784784753 37.0 30.0 40.0 2.0 41.0 22 30.839005377199403 36.0 30.0 40.0 2.0 41.0 23 30.816425912373923 37.0 30.0 40.0 2.0 41.0 24 30.549013649813865 37.0 30.0 40.0 2.0 41.0 25 29.98960991441026 36.0 28.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.313826086036034 1101 2 -0.17451598848435168 1101 3 -0.0533276418218982 1101 4 -0.10304014091488511 1101 5 0.10505930692378485 1101 6 -0.3594610688176303 1101 7 -0.29498275466254853 1101 8 -0.547918209903667 1101 9 -0.4713598826546992 1101 10 -0.1803763574097701 1101 11 -0.16309067042200454 1101 12 -0.5089896170412587 1101 13 -0.33904090558663924 1101 14 -0.5571197649526844 1101 15 -0.44593355775788623 1101 16 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0.2554776321704644 1103 3 0.1616127053571219 1103 4 0.8040472719634657 1103 5 0.0016782539752213665 1103 6 0.08050968921499546 1103 7 0.23443355506456598 1103 8 0.17686455556228253 1103 9 0.20851982112179357 1103 10 0.15884631343583067 1103 11 0.2285476325929281 1103 12 -0.011997022628928988 1103 13 0.3791544925677073 1103 14 0.46553879743612114 1103 15 0.4664594099055712 1103 16 0.5051596110052792 1103 17 0.42164686642754745 1103 18 0.1281994202718657 1103 19 0.4857745943155223 1103 20 0.4238565021335141 1103 21 0.13079764586242604 1103 22 0.189068852878016 1103 23 0.008601355446081982 1103 24 0.5449721007587698 1103 25 0.3091084189815767 1104 1 -0.006275620590830755 1104 2 0.087902300249727 1104 3 0.12090529556893514 1104 4 0.1301708028223736 1104 5 -0.022649373416474106 1104 6 -0.03343058489240747 1104 7 -0.20857448404375134 1104 8 -0.23276094737798303 1104 9 0.14885344808494594 1104 10 -0.05174975509895674 1104 11 0.30897045953386026 1104 12 0.4023265574328114 1104 13 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16 -0.6945918773337887 2313 17 -0.4873277726388494 2313 18 -0.6918071338818308 2313 19 -0.7114280564755262 2313 20 -0.6839320171739303 2313 21 -0.5916111876802752 2313 22 -0.26347725377262776 2313 23 -0.2008493700623184 2313 24 -0.42856550369805646 2313 25 -0.7846796997942498 2314 1 -0.2978765219478312 2314 2 -0.2823985822462767 2314 3 -0.2951632790362275 2314 4 -0.3293177664222924 2314 5 -0.3619139931688977 2314 6 -0.3896552094097139 2314 7 -0.4901740949116551 2314 8 -0.08099942976280872 2314 9 -0.2792365287855141 2314 10 -0.08252295660386721 2314 11 -0.20679832452760394 2314 12 -0.6240242372106657 2314 13 -0.24550945005261937 2314 14 -0.3541791570056603 2314 15 -0.26134139177932525 2314 16 0.014894356184008473 2314 17 0.13195327311278504 2314 18 0.13787107886668437 2314 19 -0.12728626967950873 2314 20 -0.3698649269231957 2314 21 -0.3671059384261035 2314 22 -0.33923275922684226 2314 23 -0.057115453731558574 2314 24 -0.19685818662488686 2314 25 -0.3158563981158551 2315 1 -0.2179772233538877 2315 2 -0.17986417701395752 2315 3 -0.28663739968325785 2315 4 -0.36088428320477917 2315 5 -0.3551789377896135 2315 6 -0.39673415489988884 2315 7 -0.37072094507289677 2315 8 -0.7529347524679899 2315 9 -0.31735642315285517 2315 10 -0.5507390753173276 2315 11 -0.5369685176900312 2315 12 -0.2529440978378439 2315 13 -0.5409370092763339 2315 14 -0.67029527507027 2315 15 -0.7529842132354752 2315 16 -1.2258384541768166 2315 17 -0.984434063944942 2315 18 -0.7124556167386622 2315 19 -0.29050754674463164 2315 20 -0.5309579783964082 2315 21 -0.5498121589440146 2315 22 -0.5789371435013315 2315 23 -0.3172001934456752 2315 24 -0.4490496750202162 2315 25 -0.6131202007618626 2316 1 -1.9661185002098307 2316 2 -1.789542739508743 2316 3 -1.820073570783066 2316 4 -1.9048092116352535 2316 5 -1.9113575419753879 2316 6 -2.3168160082032045 2316 7 -2.108439475326655 2316 8 -1.9170478462171232 2316 9 -2.5103717932298437 2316 10 -2.392295955719785 2316 11 -2.4063471830225858 2316 12 -2.3760784261960524 2316 13 -2.3176400860711333 2316 14 -2.7653159815935204 2316 15 -2.672972261819371 2316 16 -3.104125093605372 2316 17 -3.121778070170798 2316 18 -2.836451558815984 2316 19 -2.4192736325168873 2316 20 -2.272459965666762 2316 21 -2.4260663627851464 2316 22 -2.6458789706782966 2316 23 -2.842628220906139 2316 24 -2.8912520708622367 2316 25 -2.840259643214406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33001.0 3 2.0 4 543.0 5 3904.0 6 4645.0 7 3714.0 8 3065.0 9 2740.0 10 2506.0 11 2386.0 12 2338.0 13 2182.0 14 2017.0 15 2097.0 16 2208.0 17 2280.0 18 2504.0 19 2740.0 20 3355.0 21 4327.0 22 5419.0 23 6715.0 24 7761.0 25 9087.0 26 10243.0 27 11840.0 28 13536.0 29 16105.0 30 19761.0 31 24614.0 32 30499.0 33 37776.0 34 47278.0 35 59291.0 36 75922.0 37 93828.0 38 76351.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.51952737728237 23.688928552612882 15.394562699460549 20.396981370644195 2 17.48560246905724 24.761048712972094 38.563110503038594 19.19023831493207 3 21.054440166725 27.76957586941996 31.197301854974707 19.97868210888033 4 15.850647491170575 20.798943650768397 37.36803589041968 25.98237296764135 5 12.83876697415917 40.04485061466669 35.68345461143261 11.432927799741528 6 30.046135734512713 40.31610932578192 17.589805593560087 12.04794934614528 7 26.624296032326832 33.98899105921283 22.88809698049572 16.498615927964618 8 24.83394663701172 39.03665980975654 20.350863795673625 15.778529757558113 9 26.698431385026566 15.938623564224125 21.11043940309905 36.25250564765026 10 15.244042126698274 30.990168315886603 34.484393394635525 19.2813961627796 11 33.91040122180152 23.47258901014986 25.23815584332941 17.37885392471921 12 23.23666040917624 26.750294314168443 32.05669922682873 17.956346049826593 13 29.489484234305895 23.31286391549206 26.223710585764742 20.973941264437304 14 20.779057558306025 24.152693372363103 28.58808743517134 26.480161634159533 15 22.77323491043304 32.85977918482929 24.228737789939228 20.138248114798436 16 21.21082439784912 30.103407680804352 28.70231315027522 19.983454771071305 17 21.042826688726972 30.465175474879885 28.585701104075856 19.906296732317287 18 20.77969391326482 28.3702949505234 31.53759266919087 19.312418467020905 19 22.807952540571673 27.988844679825448 29.72227834528357 19.480924434319313 20 22.8432339559006 28.154411530751855 30.225428744153486 18.776925769194055 21 22.302014063444588 27.70339495370518 29.41725794648255 20.577333036367687 22 22.602850870215406 28.90721944700754 29.737344490756946 18.752585192020106 23 21.44579846638455 28.83228865060931 30.173406726271914 19.548506156734227 24 22.303274254621822 27.981035415407135 30.599643618544565 19.116046711426478 25 21.88496801485269 28.561519505292143 30.388196756780832 19.16531572307433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 982.0 1 982.0 2 771.5 3 561.0 4 561.0 5 561.0 6 1158.5 7 1756.0 8 1756.0 9 1756.0 10 2469.5 11 3183.0 12 3183.0 13 3183.0 14 4281.0 15 5379.0 16 5379.0 17 5379.0 18 8645.5 19 11912.0 20 11912.0 21 11912.0 22 17918.5 23 23925.0 24 23925.0 25 23925.0 26 32889.0 27 41853.0 28 41853.0 29 41853.0 30 50963.5 31 60074.0 32 60074.0 33 60074.0 34 65566.0 35 71058.0 36 71058.0 37 71058.0 38 72877.5 39 74697.0 40 74697.0 41 74697.0 42 75868.5 43 77040.0 44 77040.0 45 77040.0 46 75245.5 47 73451.0 48 73451.0 49 73451.0 50 69034.5 51 64618.0 52 64618.0 53 64618.0 54 56552.0 55 48486.0 56 48486.0 57 48486.0 58 40570.5 59 32655.0 60 32655.0 61 32655.0 62 26037.5 63 19420.0 64 19420.0 65 19420.0 66 14835.5 67 10251.0 68 10251.0 69 10251.0 70 7422.0 71 4593.0 72 4593.0 73 4593.0 74 3187.0 75 1781.0 76 1781.0 77 1781.0 78 1217.0 79 653.0 80 653.0 81 653.0 82 415.0 83 177.0 84 177.0 85 177.0 86 116.0 87 55.0 88 55.0 89 55.0 90 34.5 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.350472493556906 2 0.0 3 0.0 4 0.0 5 0.04327213719812911 6 0.0 7 0.0 8 0.0027045085748830695 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.590887396990041E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0063635495879601645 25 0.0011136211778930288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628580.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.01054499207063 #Duplication Level Percentage of deduplicated Percentage of total 1 91.11910338189404 73.81608224156064 2 6.606687151969518 10.704226535463231 3 1.2369547915851449 3.0061914539059718 4 0.3835323952001013 1.242806734290977 5 0.18723902566976036 0.7584167756645795 6 0.10302263269592407 0.5007551772728834 7 0.07072777221956315 0.4010786761507291 8 0.049834731576033534 0.3229711011606422 9 0.0329195829768995 0.24001500220832878 >10 0.17524323109062134 2.568359140123835 >50 0.019624537688765504 1.1341570605825697 >100 0.01236335353688012 2.122675544345873 >500 0.0015699496552903612 0.8870977783666695 >1k 0.0011774622414677707 2.2951667789031553 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4359 0.6934678163479588 No Hit GGTATCAACGCAGAGTACTTTTTTT 3387 0.538833561360527 No Hit TATCAACGCAGAGTACTTTTTTTTT 3100 0.4931750930669127 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1527 0.24292850552037926 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1055 0.16783862038244934 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1009 0.16052053835629512 No Hit CTGTAGGACGTGGAATATGGCAAGA 899 0.14302077698940469 No Hit GTCCTACAGTGGACATTTCTAAATT 884 0.14063444589391963 No Hit CTTTAGGACGTGAAATATGGCGAGG 810 0.12886187915619332 No Hit GTCCTAAAGTGTGTATTTCTCATTT 781 0.12424830570492221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.590887396990041E-4 2 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 3 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 4 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 5 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 6 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 7 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 8 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 9 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 10 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 11 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 12 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 13 0.0 0.0 0.0 1.590887396990041E-4 1.590887396990041E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAAAG 25 0.006038676 18.994272 14 GGTATCA 725 0.0 14.463525 1 TATACCT 40 0.005271103 14.251372 5 TAACCTG 55 1.9556304E-4 13.819512 5 TAGGACC 315 0.0 13.572737 4 GTGTAGA 95 7.0351234E-8 13.044853 1 TAGATTG 70 1.0879282E-4 12.215463 5 CCAGTAC 70 1.0879282E-4 12.215463 3 TCTAGAT 55 0.0030627393 12.092073 3 TAGACCT 95 1.0352851E-6 12.001157 4 CTGTAGG 665 0.0 11.7547035 1 TGTAGGA 755 0.0 11.451434 2 ACAATAC 75 2.0682185E-4 11.401098 3 AACAGGT 75 2.0758764E-4 11.396563 7 GTAGGAC 735 0.0 11.375245 3 AGTAGAG 60 0.0058648535 11.084401 5 CACCTTT 180 3.6379788E-12 11.079992 14 GTTCTAA 105 3.3151573E-6 10.894603 1 AAGGCGT 70 0.0014930125 10.85387 6 CATACTG 80 3.7605915E-4 10.68853 5 >>END_MODULE