FastQCFastQC Report
Thu 26 May 2016
SRR1512061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262980
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43020.3406229710684255No Hit
GGTATCAACGCAGAGTACTTTTTTT26010.2059415034283995No Hit
TATCAACGCAGAGTACTTTTTTTTT25680.20332863544949248No Hit
GTCCTACAGTGGACATTTCTAAATT22560.17862515637618964No Hit
GTCCTAAAGTGTGTATTTCTCATTT20130.1593849467133288No Hit
CTGTAGGACGTGGAATATGGCAAGA18380.14552882864336728No Hit
CTTTAGGACGTGAAATATGGCGAGG16680.13206859966111895No Hit
GTCCTACAGTGTGCATTTCTCATTT13410.10617745332467657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA551.454919E-414.3114042
ACCGTCC655.6617835E-513.1007288
GGTATCA7850.013.0352281
TAGGACC4600.012.7526424
GGCGAGG5250.012.43529519
CCTAGAC1052.864599E-711.7144083
AGGGCCG658.298502E-411.6450925
CGGTAGG600.004523273611.4789381
GACGTGG5300.011.4253747
GGACGTG11050.011.3882156
TCTATAC1105.231286E-711.1819353
TGTACCG600.006054212311.0385775
AGGACGT11700.010.9981425
GTCCAAG1209.699961E-710.6590141
CTAGGAC1701.4915713E-1010.5747723
GTCCCGG750.001977885110.4950291
TAGCCCT2800.010.4753854
GACGTGA6550.010.40057857
GTAGGAC13000.010.3350193
TGTAGGA12700.010.3039282
Produced by FastQC (version 0.11.5)