FastQCFastQC Report
Thu 26 May 2016
SRR1512061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262980
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54710.4331818397757684No Hit
TATCAACGCAGAGTACTTTTTTTTT38570.3053888422619519No Hit
GGTATCAACGCAGAGTACTTTTTTT37340.2956499707042075No Hit
CTGTAGGACGTGGAATATGGCAAGA19280.15265483222220463No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18440.1460038955486231No Hit
CTTTAGGACGTGAAATATGGCGAGG17140.13571077926808026No Hit
GTCCTACAGTGGACATTTCTAAATT16960.1342855785523128No Hit
GTCCTAAAGTGTGTATTTCTCATTT16360.12953490949975455No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12700.1005558282791493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG400.00527334214.2520494
GGTATCA11500.013.7569711
TCCAACG1205.2750693E-1113.45547318
CCAACGA802.8704395E-511.87294619
TAGGTTG907.44542E-611.6127825
CCATGCG855.336632E-511.173219
CCAACGT700.001493220310.85526519
CTATAGT1502.1245796E-910.7682154
ACTTATC803.7784126E-410.6848058
CTGTGCG909.539093E-510.5524769
TAGGACC4000.010.4515034
ATAGGAC2250.010.1347913
CCCGTTT750.00265339910.13037712
TAGGACT2350.010.1078374
TTAGACT856.582409E-410.060274
TAAGCCC856.582409E-410.060274
GGCGAGG5700.09.9982719
TGGCGAG12100.09.89045918
CTACTCT1451.6116246E-79.8294
TACACTG6100.09.8128875
Produced by FastQC (version 0.11.5)