##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512061_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1262980 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.887545329300544 31.0 29.0 33.0 24.0 34.0 2 29.095333259434035 31.0 29.0 34.0 25.0 34.0 3 29.125035234128806 31.0 28.0 34.0 25.0 34.0 4 32.671191151087115 35.0 32.0 37.0 26.0 37.0 5 32.10168252862278 35.0 32.0 37.0 23.0 37.0 6 32.047170184801026 35.0 32.0 37.0 23.0 37.0 7 31.774081933205593 35.0 32.0 37.0 19.0 37.0 8 31.616014505376175 35.0 32.0 37.0 18.0 37.0 9 32.79864922643272 37.0 32.0 39.0 17.0 39.0 10 32.48523492058465 37.0 32.0 39.0 17.0 39.0 11 32.509430869847506 37.0 32.0 39.0 17.0 39.0 12 32.21643098069645 37.0 32.0 39.0 15.0 39.0 13 32.19104023816688 37.0 32.0 39.0 11.0 39.0 14 32.81724334510444 38.0 32.0 40.0 10.0 41.0 15 32.718579866664555 38.0 32.0 40.0 10.0 41.0 16 32.55405469603636 37.0 32.0 40.0 10.0 41.0 17 32.46624807993793 37.0 31.0 40.0 10.0 41.0 18 32.344075123913285 37.0 31.0 40.0 10.0 41.0 19 32.35322095361764 37.0 31.0 40.0 8.0 41.0 20 32.192665758761024 37.0 31.0 40.0 2.0 41.0 21 32.00286940410775 37.0 31.0 40.0 2.0 41.0 22 31.82595923925953 37.0 31.0 40.0 2.0 41.0 23 31.827656811667644 37.0 31.0 40.0 2.0 41.0 24 31.606457742798778 37.0 31.0 40.0 2.0 41.0 25 31.06033349696749 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38805580150076935 1101 2 0.33080064392475705 1101 3 0.2159942378461004 1101 4 0.2912311555604532 1101 5 0.40785867618364335 1101 6 -0.07934479788606197 1101 7 0.1663686270313498 1101 8 0.00402867768532289 1101 9 -0.0959041343292526 1101 10 0.2908801726406267 1101 11 0.06803067629572013 1101 12 -0.34520274210761315 1101 13 0.26463574283014424 1101 14 -0.17448232453530466 1101 15 0.04940979046339322 1101 16 -0.02298784034945811 1101 17 -0.3178467001648997 1101 18 -0.07807794429127313 1101 19 -0.29223842553901136 1101 20 -0.4103745963792029 1101 21 -0.30805916906409436 1101 22 -0.6098134799194028 1101 23 -0.47286649903639244 1101 24 -0.6376794573833742 1101 25 -0.41821114493614076 1102 1 0.4410011474806481 1102 2 0.4825159912617032 1102 3 0.5061177984830039 1102 4 0.66787744375047 1102 5 0.42103515020249205 1102 6 0.3055297068115266 1102 7 0.6902788495795669 1102 8 0.665099394476087 1102 9 0.7217567260978086 1102 10 0.9179119920000645 1102 11 0.5656692628347102 1102 12 0.9228622671395286 1102 13 0.8438050591473925 1102 14 1.1825245853047974 1102 15 1.1349248096362103 1102 16 0.8860806378370469 1102 17 0.9335835503770866 1102 18 0.7554011899206756 1102 19 0.6751038599682673 1102 20 1.335985708995132 1102 21 1.1362067627661432 1102 22 1.0350716570268794 1102 23 1.2839813756914893 1102 24 1.1636803040415273 1102 25 1.169623229751931 1103 1 0.2637162449311319 1103 2 0.3028357236845203 1103 3 0.3074773531789603 1103 4 0.4853249908344637 1103 5 0.46466286778326804 1103 6 0.4567139267946416 1103 7 0.5572256012479038 1103 8 0.47895550308599155 1103 9 0.4063290949775862 1103 10 0.4890440352904122 1103 11 0.19499108382703412 1103 12 0.29156933856173595 1103 13 0.07599724186879797 1103 14 0.29124068895877286 1103 15 0.43935607403065546 1103 16 0.40243573541626176 1103 17 0.17937717997191527 1103 18 0.29237273195434454 1103 19 0.39548387878362945 1103 20 0.31157293814885634 1103 21 0.48689227912124267 1103 22 0.3637875032300677 1103 23 0.19976278710106854 1103 24 0.2683828572024325 1103 25 0.29629008950668734 1104 1 0.44691393388123757 1104 2 0.16026913242948027 1104 3 0.18756643115114713 1104 4 0.2458523144082676 1104 5 0.36708097429709596 1104 6 0.27374847990834894 1104 7 0.3229469944428338 1104 8 0.1861636960281956 1104 9 0.5650678864821401 1104 10 0.30372130463864266 1104 11 0.4917594328273225 1104 12 0.7380876921210486 1104 13 0.4262145196056366 1104 14 0.6925024123557932 1104 15 0.6064695250833623 1104 16 0.9592513695443685 1104 17 0.7318097144569151 1104 18 0.7701831559221546 1104 19 0.7135369715721112 1104 20 1.2214254724836735 1104 21 1.0756007021600809 1104 22 0.9870302675220692 1104 23 1.1295098309760476 1104 24 0.7446115678996179 1104 25 0.8333335032183022 1105 1 0.1368249030493125 1105 2 0.18853964240730647 1105 3 0.05390660622929744 1105 4 0.386992966848986 1105 5 0.07576436221521021 1105 6 0.24574580830532256 1105 7 0.44152141621707486 1105 8 0.18490926313357292 1105 9 0.14988111159520656 1105 10 0.3706368236687325 1105 11 0.2647748860844388 1105 12 0.19079726717342993 1105 13 0.17428710041484408 1105 14 0.5233048425652527 1105 15 0.1265828308869814 1105 16 0.25781826472124436 1105 17 0.5612085359680634 1105 18 0.5588383360519416 1105 19 0.47232817291198614 1105 20 0.5110265628231829 1105 21 0.5468792744120279 1105 22 0.4739502618262712 1105 23 0.2923114881836071 1105 24 0.513753535799701 1105 25 0.4708583375992532 1106 1 -0.08425043239160246 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-1.8282311718308684 2315 15 -1.5187293260135704 2315 16 -1.718626033334104 2315 17 -1.7152593271617 2315 18 -1.8926818790329207 2315 19 -1.6556931106347754 2315 20 -1.767124328632871 2315 21 -1.747492689576255 2315 22 -1.854552231251489 2315 23 -1.7966761240852236 2315 24 -1.9339065594246243 2315 25 -1.7887096181840967 2316 1 -1.6755954588263258 2316 2 -1.8592034131482507 2316 3 -1.8945754911203458 2316 4 -1.9538582054242042 2316 5 -2.3024689456339225 2316 6 -2.135744698830255 2316 7 -2.11510925401738 2316 8 -2.1844921767237153 2316 9 -2.6690934679154985 2316 10 -2.8267064485025237 2316 11 -2.861077241846246 2316 12 -2.8658095116237163 2316 13 -2.8078659959154315 2316 14 -2.8032035035101828 2316 15 -2.9911780636084337 2316 16 -2.883849994707912 2316 17 -2.988549364133874 2316 18 -3.0506163020724237 2316 19 -3.1274106140401052 2316 20 -3.199090664495877 2316 21 -3.2942506312964355 2316 22 -3.294756733586574 2316 23 -3.4716888940793886 2316 24 -3.1267121879515756 2316 25 -3.318395293899215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53426.0 3 2.0 4 924.0 5 7012.0 6 8313.0 7 6715.0 8 5378.0 9 4867.0 10 4550.0 11 4335.0 12 4033.0 13 3822.0 14 3746.0 15 3707.0 16 3838.0 17 3971.0 18 4295.0 19 4865.0 20 5841.0 21 7519.0 22 9507.0 23 11661.0 24 14078.0 25 16465.0 26 18503.0 27 21009.0 28 24278.0 29 29197.0 30 36111.0 31 45293.0 32 56384.0 33 71014.0 34 89318.0 35 116967.0 36 158399.0 37 216464.0 38 187173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94500429157262 22.44230219029151 15.322583208824744 22.290110309311125 2 16.55362713582163 23.181047997592994 38.76419262379452 21.50113224279086 3 20.179100223281445 26.31490601593058 30.895026049501972 22.610967711286005 4 14.956927267256805 19.617254429998894 36.881581656083235 28.54423664666107 5 13.30867604794783 38.91951025529717 35.41784830462019 12.353965392134807 6 31.324011464947983 38.795626217358944 17.327986191388618 12.552376126304454 7 27.55166352594657 32.809387322047854 22.279054300147273 17.359894851858304 8 25.07345948860636 37.97738770061309 20.215592018946253 16.733560791834297 9 26.365579819157865 15.671982137484363 20.516239370377995 37.44619867297978 10 15.321382761405564 30.101664317724747 33.982010799854315 20.59494212101538 11 34.08612962992288 23.04074490490744 24.88558805365089 17.98753741151879 12 23.355318374004337 25.478946618315412 31.681736844605616 19.483998163074634 13 29.0945224785824 22.507086414670066 26.09384154935153 22.304549557396 14 21.016405643794833 23.067190295966682 27.82118481686171 28.095219243376775 15 22.8766884669591 31.923862610651 23.718665378707502 21.480783543682403 16 21.93589763891748 29.172354273226812 27.828627531710715 21.06312055614499 17 21.44143216836371 29.621213320876024 27.980886474845207 20.95646803591506 18 21.46391866854582 27.928233226179355 30.344344328492927 20.263503776781896 19 23.341797448730343 27.523735549047135 28.46500218926839 20.669464812954136 20 23.471393054521844 27.831477933142253 28.716765111086477 19.980363901249426 21 22.756813251199546 27.195759236092414 28.205513943213667 21.84191356949437 22 23.198783828722544 28.254683367907646 28.216598837669636 20.32993396570017 23 22.095124229995726 28.27788246844764 28.60354083200051 21.02345246955613 24 22.974753505401818 27.38718893221042 29.024679549104132 20.61337801328363 25 22.48300227322574 28.029799764522 28.714142852957686 20.77305510929457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 508.0 1 508.0 2 547.5 3 587.0 4 587.0 5 587.0 6 1323.0 7 2059.0 8 2059.0 9 2059.0 10 2934.5 11 3810.0 12 3810.0 13 3810.0 14 5709.5 15 7609.0 16 7609.0 17 7609.0 18 13152.0 19 18695.0 20 18695.0 21 18695.0 22 28679.0 23 38663.0 24 38663.0 25 38663.0 26 54658.5 27 70654.0 28 70654.0 29 70654.0 30 88453.0 31 106252.0 32 106252.0 33 106252.0 34 118383.0 35 130514.0 36 130514.0 37 130514.0 38 138274.5 39 146035.0 40 146035.0 41 146035.0 42 151755.0 43 157475.0 44 157475.0 45 157475.0 46 157535.0 47 157595.0 48 157595.0 49 157595.0 50 150619.0 51 143643.0 52 143643.0 53 143643.0 54 127213.5 55 110784.0 56 110784.0 57 110784.0 58 93643.5 59 76503.0 60 76503.0 61 76503.0 62 61948.0 63 47393.0 64 47393.0 65 47393.0 66 36418.0 67 25443.0 68 25443.0 69 25443.0 70 18635.5 71 11828.0 72 11828.0 73 11828.0 74 8224.5 75 4621.0 76 4621.0 77 4621.0 78 3143.5 79 1666.0 80 1666.0 81 1666.0 82 1064.5 83 463.0 84 463.0 85 463.0 86 302.0 87 141.0 88 141.0 89 141.0 90 86.0 91 31.0 92 31.0 93 31.0 94 18.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3721357424503951 2 0.0 3 0.0 4 0.0 5 0.04814011306592345 6 0.0 7 0.0 8 0.003087934884162853 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 7.917781754263726E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0057008028630698825 25 0.0013460228982248334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1262980.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.09089208995167 #Duplication Level Percentage of deduplicated Percentage of total 1 87.61402212970518 67.5424312556774 2 8.802876555299587 13.572432132115317 3 1.925934286215221 4.4541597669286705 4 0.687236457524246 2.119186863491292 5 0.30951312208019954 1.193032134735435 6 0.16480167222953682 0.7622824758054484 7 0.11081133653270926 0.5979781350890486 8 0.07377732681098569 0.4550047951896636 9 0.04512083028617668 0.31305645527406023 >10 0.2345099739035909 3.1561908648938095 >50 0.014287150000125405 0.7620536318092315 >100 0.014138140845611883 2.1224129093875876 >500 0.0018440804897787966 0.9445862489505334 >1k 0.0010244891609882203 1.5730994069060806 >5k 1.0244891609882204E-4 0.4320929237465191 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5471 0.4331818397757684 No Hit TATCAACGCAGAGTACTTTTTTTTT 3857 0.3053888422619519 No Hit GGTATCAACGCAGAGTACTTTTTTT 3734 0.2956499707042075 No Hit CTGTAGGACGTGGAATATGGCAAGA 1928 0.15265483222220463 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1844 0.1460038955486231 No Hit CTTTAGGACGTGAAATATGGCGAGG 1714 0.13571077926808026 No Hit GTCCTACAGTGGACATTTCTAAATT 1696 0.1342855785523128 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1636 0.12953490949975455 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1270 0.1005558282791493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5835563508527451E-4 0.0 0.0 0.0 0.0 7 1.5835563508527451E-4 0.0 0.0 0.0 0.0 8 2.3753345262791177E-4 0.0 0.0 0.0 0.0 9 2.3753345262791177E-4 0.0 0.0 0.0 0.0 10 2.3753345262791177E-4 0.0 0.0 0.0 0.0 11 2.3753345262791177E-4 0.0 0.0 0.0 0.0 12 2.3753345262791177E-4 0.0 0.0 0.0 0.0 13 2.3753345262791177E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAACG 40 0.005273342 14.252049 4 GGTATCA 1150 0.0 13.756971 1 TCCAACG 120 5.2750693E-11 13.455473 18 CCAACGA 80 2.8704395E-5 11.872946 19 TAGGTTG 90 7.44542E-6 11.612782 5 CCATGCG 85 5.336632E-5 11.17321 9 CCAACGT 70 0.0014932203 10.855265 19 CTATAGT 150 2.1245796E-9 10.768215 4 ACTTATC 80 3.7784126E-4 10.684805 8 CTGTGCG 90 9.539093E-5 10.552476 9 TAGGACC 400 0.0 10.451503 4 ATAGGAC 225 0.0 10.134791 3 CCCGTTT 75 0.002653399 10.130377 12 TAGGACT 235 0.0 10.107837 4 TTAGACT 85 6.582409E-4 10.06027 4 TAAGCCC 85 6.582409E-4 10.06027 4 GGCGAGG 570 0.0 9.99827 19 TGGCGAG 1210 0.0 9.890459 18 CTACTCT 145 1.6116246E-7 9.829 4 TACACTG 610 0.0 9.812887 5 >>END_MODULE