FastQCFastQC Report
Thu 26 May 2016
SRR1512060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732681
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34160.46623291719042803No Hit
TATCAACGCAGAGTACTTTTTTTTT20760.28334295552907746No Hit
GGTATCAACGCAGAGTACTTTTTTT19610.2676471752372451No Hit
GTCCTACAGTGGACATTTCTAAATT12460.17006036733585284No Hit
CTGTAGGACGTGGAATATGGCAAGA12200.16651175613943858No Hit
GTCCTAAAGTGTGTATTTCTCATTT11660.15914156365457818No Hit
CTTTAGGACGTGAAATATGGCGAGG10310.1407160824424272No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8860.12092575077011687No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8820.12037981058605314No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7500.10236378451194995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4700.015.0570071
GCCTTAT450.00918271613.1051721
CCCTTGG707.925095E-512.6371311
GTATAAC707.925095E-512.6371311
TGTTGGA550.002375177312.5094822
GTATAAT1102.3648681E-812.5094821
ATTTAGA1950.012.0970821
TCCAACG550.00315598912.04417618
GGGGGCG550.003157602612.0433557
TAGGACC1650.012.0433544
AATGTCC2200.011.6132358
GTCCTAG853.7549242E-511.5633871
TAGAAAT2400.011.4340174
CACCTTT2800.011.15239114
TAAGATA600.006045358311.03974154
CTATGCA951.417955E-510.9567379
AACCATC1408.8402885E-910.8144417
CTAGAAC1053.6245783E-610.814443
GTCCTAC5500.010.7224131
GTATCAA10350.010.6360821
Produced by FastQC (version 0.11.5)