##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512060_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732681 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.688960134082908 31.0 30.0 34.0 25.0 34.0 2 27.29629129184461 31.0 27.0 34.0 10.0 34.0 3 28.911823836021405 31.0 28.0 34.0 16.0 34.0 4 33.18793308411164 35.0 33.0 37.0 28.0 37.0 5 33.57113941810965 37.0 35.0 37.0 28.0 37.0 6 33.82601568759119 37.0 35.0 37.0 30.0 37.0 7 33.891599481902766 37.0 35.0 37.0 30.0 37.0 8 33.92865107734471 37.0 35.0 37.0 30.0 37.0 9 35.57315530223931 39.0 37.0 39.0 30.0 39.0 10 35.461345387692596 39.0 37.0 39.0 30.0 39.0 11 35.48260839301142 39.0 37.0 39.0 30.0 39.0 12 35.40790603277552 39.0 37.0 39.0 30.0 39.0 13 35.39109380480728 39.0 37.0 39.0 30.0 39.0 14 36.5852178506062 40.0 37.0 41.0 30.0 41.0 15 36.52144384800479 40.0 37.0 41.0 30.0 41.0 16 36.4980625947718 40.0 37.0 41.0 30.0 41.0 17 36.459126140844376 40.0 37.0 41.0 30.0 41.0 18 36.42619775864257 40.0 37.0 41.0 30.0 41.0 19 36.45464670163413 40.0 37.0 41.0 30.0 41.0 20 36.39660916551678 40.0 37.0 41.0 30.0 41.0 21 36.33896197663103 40.0 37.0 41.0 30.0 41.0 22 36.25281943983808 40.0 37.0 41.0 29.0 41.0 23 36.15410799515751 40.0 37.0 41.0 29.0 41.0 24 36.07165328430791 40.0 37.0 41.0 29.0 41.0 25 35.96722721075066 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4603829367592951 1101 2 2.8741531207886766 1101 3 1.2442105139149682 1101 4 0.5401498023057698 1101 5 0.13666487161133034 1101 6 -0.08079364437890746 1101 7 -0.3110399698117021 1101 8 -0.28698826845935343 1101 9 -0.09283250728455528 1101 10 -0.39202882602893396 1101 11 -0.10735006625705523 1101 12 -0.3825822036673401 1101 13 -0.2210506824979106 1101 14 -0.0872766856154783 1101 15 -0.08771544104652662 1101 16 -0.1824106267302099 1101 17 -0.22125566461551927 1101 18 -0.26771131457186215 1101 19 -0.19079102325974873 1101 20 -0.04231938600953811 1101 21 0.04505334170624309 1101 22 -0.02106286110775102 1101 23 -0.11779139214799983 1101 24 -0.2317698671121846 1101 25 -0.3461480281800604 1102 1 1.5024223745567369 1102 2 4.17173308897452 1102 3 2.7192654700970067 1102 4 1.6606696077625216 1102 5 1.4832497491072232 1102 6 1.1776114667909994 1102 7 1.1174288023381749 1102 8 1.1069258855747393 1102 9 1.0726827742903424 1102 10 1.2669668395369769 1102 11 1.1361980943031895 1102 12 1.2752065490151452 1102 13 1.233517784902375 1102 14 1.403891129169935 1102 15 1.4610069297941948 1102 16 1.486511448180046 1102 17 1.234482152750303 1102 18 1.1280858848676374 1102 19 1.313445420609817 1102 20 1.2676743099529375 1102 21 1.4418778052914476 1102 22 1.381059607069922 1102 23 1.2743818023005318 1102 24 1.2296138201291598 1102 25 1.364563609465172 1103 1 1.0739632397699275 1103 2 2.845004250911881 1103 3 1.8873681080053508 1103 4 1.158426510473035 1103 5 0.8984107529256775 1103 6 0.7719820057178097 1103 7 0.5124959331833523 1103 8 0.4257131219621826 1103 9 0.6338055546937085 1103 10 0.5723894948195962 1103 11 0.4652251346465235 1103 12 0.38301622285842285 1103 13 0.4966684289824812 1103 14 0.5148887888285429 1103 15 0.5580938206409343 1103 16 0.3805283427220587 1103 17 0.4311000028776917 1103 18 0.3813435622895014 1103 19 0.5159384352418144 1103 20 0.6294450602235955 1103 21 0.5428722883093826 1103 22 0.6352459656672735 1103 23 0.5083883889677168 1103 24 0.5646521667201085 1103 25 0.597917039912943 1104 1 0.96092544625321 1104 2 3.63947901399181 1104 3 2.091029083239931 1104 4 1.1680270401574333 1104 5 0.8920705912114215 1104 6 0.6364426572563744 1104 7 0.6279664628008703 1104 8 0.38758353654723265 1104 9 0.5741441648072083 1104 10 0.43425188388349056 1104 11 0.2456686115698119 1104 12 0.3089623983631995 1104 13 0.35164558968685355 1104 14 0.22966511086595887 1104 15 0.2588706242096137 1104 16 0.34246730863365826 1104 17 0.3961588901391835 1104 18 0.47153537224516384 1104 19 0.5512457123831211 1104 20 0.1702280835711747 1104 21 0.24609185988030902 1104 22 0.4323975744498654 1104 23 0.5928185435258797 1104 24 0.6561631717159955 1104 25 0.6272302559399137 1105 1 0.9273294878299474 1105 2 3.584208130195936 1105 3 1.8811330602597955 1105 4 1.1835259662618043 1105 5 0.7916626691862731 1105 6 0.6375913122042931 1105 7 0.2828123618564433 1105 8 0.21054467401466326 1105 9 0.3601055960347921 1105 10 0.4053156298354921 1105 11 0.20901719580421485 1105 12 0.08657189437984414 1105 13 0.2614665015394593 1105 14 0.2781636798846421 1105 15 0.31089959212643237 1105 16 0.29385440183302336 1105 17 0.40383183482960305 1105 18 0.41452655416360784 1105 19 0.2685341127271883 1105 20 0.41972719613408316 1105 21 0.2779674744458873 1105 22 0.46299260585757906 1105 23 0.255555508578972 1105 24 0.2519509678288614 1105 25 0.4748426191963233 1106 1 0.31102069393915244 1106 2 3.0270389109396874 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0.06551538565612702 1107 15 0.04662868288400546 1107 16 0.23164839719115093 1107 17 0.25093740454008184 1107 18 0.3360509594352692 1107 19 0.1296645797332232 1107 20 0.3348029855443144 1107 21 0.13443834219873452 1107 22 0.5088244099274561 1107 23 0.12247855958811016 1107 24 0.35702244870552846 1107 25 0.45447726264301025 1108 1 0.5303792633210271 1108 2 2.121815635776997 1108 3 1.2677967600076094 1108 4 0.7703011063763583 1108 5 0.5234640592963018 1108 6 0.5674815723886084 1108 7 0.35074033085575707 1108 8 0.24535531584604797 1108 9 0.28612077073927367 1108 10 0.2519838161435217 1108 11 0.478393527751777 1108 12 0.35977808438566683 1108 13 0.401181243146155 1108 14 0.2735450555942549 1108 15 0.2123074313049571 1108 16 0.3383165754267168 1108 17 0.18378595613316406 1108 18 0.34610289262392513 1108 19 0.1886751499186019 1108 20 0.2762404875687352 1108 21 0.47676551049033833 1108 22 0.4763310770260034 1108 23 0.3386437092874175 1108 24 0.35415046779449 1108 25 0.43781221551444816 1109 1 1.1176600831490902 1109 2 3.6554271507000955 1109 3 2.0678703435248984 1109 4 1.4806674087148792 1109 5 0.9642795800342938 1109 6 0.46232893145822374 1109 7 0.5495450473797447 1109 8 0.5782579163952377 1109 9 0.5215270055963259 1109 10 0.5843587690321286 1109 11 0.5219854802275918 1109 12 0.49515021956397476 1109 13 0.4848672389465136 1109 14 0.6481156688500604 1109 15 0.6320996027366093 1109 16 0.7067528359482651 1109 17 0.6691374520322313 1109 18 0.5183170386064404 1109 19 0.37468889747329825 1109 20 0.5595657446623648 1109 21 0.5427142891834222 1109 22 0.49248535115152237 1109 23 0.4796476756184731 1109 24 0.5905976402132325 1109 25 0.617885440087754 1110 1 -0.44888282040493266 1110 2 1.0975970512730946 1110 3 0.39283419636580064 1110 4 -0.19661610996561762 1110 5 -0.42268602215060724 1110 6 -0.4625296037339339 1110 7 -0.5922285732268193 1110 8 -0.3602919131329756 1110 9 -0.44854493171290244 1110 10 -0.5830588065199009 1110 11 -0.5668222882877885 1110 12 -0.6035306322594494 1110 13 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-1.0603138054000283 2314 11 -1.1655873389909885 2314 12 -1.0102066183266842 2314 13 -0.9744564673856786 2314 14 -0.8753705453096003 2314 15 -1.0412509352242623 2314 16 -0.9035319891907321 2314 17 -1.1464529766286518 2314 18 -0.9860113167703233 2314 19 -0.6902743845624144 2314 20 -0.7413242152989241 2314 21 -0.828784221224744 2314 22 -0.8525800201078937 2314 23 -0.9229709698966246 2314 24 -0.6947037886563834 2314 25 -0.8575030872087765 2315 1 -1.2904155682995224 2315 2 -25.120731789697256 2315 3 -13.863631329690588 2315 4 -6.945649808823234 2315 5 -3.836389706160926 2315 6 -2.816359428388626 2315 7 -2.0916150486919705 2315 8 -2.1602481483828235 2315 9 -2.0482365333800487 2315 10 -1.9735364967227298 2315 11 -1.802160074445851 2315 12 -2.0183591112503194 2315 13 -1.9434096003343555 2315 14 -1.8974148861751132 2315 15 -1.85388055986175 2315 16 -1.9247719507544474 2315 17 -1.9582108280228425 2315 18 -2.1141945935072357 2315 19 -1.9669786903830797 2315 20 -1.931822973946005 2315 21 -1.8240927332969576 2315 22 -1.8875466057568957 2315 23 -1.9490632952484859 2315 24 -2.103395867911665 2315 25 -2.1153849173673507 2316 1 -0.9219965271035448 2316 2 -1.2940651230305882 2316 3 -0.661832869622021 2316 4 -0.7906203028377377 2316 5 -0.7333019750307059 2316 6 -0.8695915983430993 2316 7 -1.1814728579428007 2316 8 -1.2699834375414838 2316 9 -1.4484411082128474 2316 10 -1.6769412064400342 2316 11 -1.7735847920314214 2316 12 -1.6050999643948174 2316 13 -1.8820888562215075 2316 14 -1.8836184493741257 2316 15 -2.1551361097971196 2316 16 -2.0653148608876464 2316 17 -2.0659520745804727 2316 18 -1.8192448941872783 2316 19 -2.1203221400542986 2316 20 -2.1098480399832056 2316 21 -2.059169448859258 2316 22 -2.049540648357919 2316 23 -2.092106064876994 2316 24 -1.999453868248871 2316 25 -2.3670839001133714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26597.0 3 2.0 4 198.0 5 1063.0 6 1218.0 7 1110.0 8 950.0 9 909.0 10 863.0 11 836.0 12 785.0 13 795.0 14 823.0 15 790.0 16 848.0 17 943.0 18 1024.0 19 1319.0 20 1615.0 21 2199.0 22 2538.0 23 2945.0 24 3277.0 25 3828.0 26 4337.0 27 4978.0 28 5893.0 29 7091.0 30 9450.0 31 12610.0 32 16969.0 33 23848.0 34 34333.0 35 52014.0 36 82743.0 37 126069.0 38 294871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.337089401799695 19.15444784292209 13.57684995243496 25.931612802843258 2 15.3398394084424 21.08969197269036 40.52881234831129 23.041656270555947 3 17.558528199857783 24.971031048983118 30.774784660718645 26.69565609044045 4 11.828067057832808 17.67303915346515 38.98299532811688 31.515898460585166 5 12.490974926332196 37.73634091780734 36.36603105580737 13.406653100053093 6 29.434910963980233 38.09952762525574 19.067916323747987 13.397645087016041 7 25.874698538654613 32.32771151428794 23.725332033995695 18.072257913061758 8 23.641217560783865 36.68965084252564 21.808625344284938 17.860506252405557 9 25.10915391555124 15.66848328262914 21.617593468371638 37.604769333447976 10 14.653443995408644 28.457405064414115 34.899226266274134 21.989924673903104 11 31.76211748359791 22.988039815417626 26.283471251472335 18.966371449512135 12 22.34383039822242 24.97335129476539 32.622792183774386 20.060026123237805 13 27.203243976573706 22.584317049302495 27.065667050189646 23.146771923934153 14 20.092619257872162 22.6277063426312 28.775026375879136 28.504648023617502 15 21.73456115280729 31.040657530357684 24.999146968462398 22.22563434837262 16 20.837308460298548 28.41345687959699 28.88583162385813 21.863403036246336 17 20.27471710062087 28.75098439839439 29.260073620033822 21.71422488095092 18 20.091690653913506 27.477169463927687 31.41954001809792 21.011599864060894 19 21.78669844038538 27.02963499804144 29.629811609690986 21.553854951882197 20 21.961126329193743 27.332768285242825 30.04254238884317 20.663562996720263 21 21.31309533071009 26.82422500378746 29.154843649555534 22.707836015946913 22 21.602093140379345 27.970775742173586 29.41861306247347 21.008518054973596 23 20.70060695065643 27.84684698210267 29.760782006854296 21.69176406038661 24 21.41177401897961 26.896562078175908 30.252729359707704 21.43893454313678 25 21.07683971605651 27.36129365986016 30.060421929871257 21.50144469421208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 595.0 1 595.0 2 531.0 3 467.0 4 467.0 5 467.0 6 1005.0 7 1543.0 8 1543.0 9 1543.0 10 2088.5 11 2634.0 12 2634.0 13 2634.0 14 3727.0 15 4820.0 16 4820.0 17 4820.0 18 8352.0 19 11884.0 20 11884.0 21 11884.0 22 17763.0 23 23642.0 24 23642.0 25 23642.0 26 33068.0 27 42494.0 28 42494.0 29 42494.0 30 52540.5 31 62587.0 32 62587.0 33 62587.0 34 69070.5 35 75554.0 36 75554.0 37 75554.0 38 79711.0 39 83868.0 40 83868.0 41 83868.0 42 87068.5 43 90269.0 44 90269.0 45 90269.0 46 90694.5 47 91120.0 48 91120.0 49 91120.0 50 87140.5 51 83161.0 52 83161.0 53 83161.0 54 72986.0 55 62811.0 56 62811.0 57 62811.0 58 53487.0 59 44163.0 60 44163.0 61 44163.0 62 35360.5 63 26558.0 64 26558.0 65 26558.0 66 20502.0 67 14446.0 68 14446.0 69 14446.0 70 10416.0 71 6386.0 72 6386.0 73 6386.0 74 4427.0 75 2468.0 76 2468.0 77 2468.0 78 1643.5 79 819.0 80 819.0 81 819.0 82 557.0 83 295.0 84 295.0 85 295.0 86 181.5 87 68.0 88 68.0 89 68.0 90 45.5 91 23.0 92 23.0 93 23.0 94 14.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.724540420728803 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.094551380477998E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.459401840637331E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 2.7297009203186654E-4 23 2.7297009203186654E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732681.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.19904019351807 #Duplication Level Percentage of deduplicated Percentage of total 1 89.09193828907569 70.55996001474936 2 7.852949394329502 12.438921094383312 3 1.638231832234221 3.8923916618225656 4 0.5423395850685135 1.7181109838550848 5 0.26315624939781834 1.0420861186616641 6 0.15647155419118566 0.7435438145717973 7 0.09348499150793581 0.5182745119949392 8 0.06586216682393291 0.41729763180166846 9 0.04109922996024372 0.29295176089895864 >10 0.22217166615392225 3.0954281285457443 >50 0.016060528031305598 0.9141581651770099 >100 0.014139737331917875 2.1846823406501747 >500 8.728232890829815E-4 0.5071139609067695 >1k 0.0012219526047161742 1.6750798119810304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3416 0.46623291719042803 No Hit TATCAACGCAGAGTACTTTTTTTTT 2076 0.28334295552907746 No Hit GGTATCAACGCAGAGTACTTTTTTT 1961 0.2676471752372451 No Hit GTCCTACAGTGGACATTTCTAAATT 1246 0.17006036733585284 No Hit CTGTAGGACGTGGAATATGGCAAGA 1220 0.16651175613943858 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1166 0.15914156365457818 No Hit CTTTAGGACGTGAAATATGGCGAGG 1031 0.1407160824424272 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 886 0.12092575077011687 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 882 0.12037981058605314 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 750 0.10236378451194995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.3648504601593327E-4 3 0.0 0.0 0.0 0.0 1.3648504601593327E-4 4 0.0 0.0 0.0 0.0 1.3648504601593327E-4 5 0.0 0.0 0.0 0.0 1.3648504601593327E-4 6 0.0 0.0 0.0 0.0 1.3648504601593327E-4 7 0.0 0.0 0.0 0.0 1.3648504601593327E-4 8 0.0 0.0 0.0 0.0 1.3648504601593327E-4 9 0.0 0.0 0.0 0.0 1.3648504601593327E-4 10 0.0 0.0 0.0 0.0 1.3648504601593327E-4 11 0.0 0.0 0.0 0.0 1.3648504601593327E-4 12 0.0 0.0 0.0 0.0 2.7297009203186654E-4 13 0.0 0.0 0.0 0.0 2.7297009203186654E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 470 0.0 15.057007 1 GCCTTAT 45 0.009182716 13.105172 1 CCCTTGG 70 7.925095E-5 12.637131 1 GTATAAC 70 7.925095E-5 12.637131 1 TGTTGGA 55 0.0023751773 12.509482 2 GTATAAT 110 2.3648681E-8 12.509482 1 ATTTAGA 195 0.0 12.097082 1 TCCAACG 55 0.003155989 12.044176 18 GGGGGCG 55 0.0031576026 12.043355 7 TAGGACC 165 0.0 12.043354 4 AATGTCC 220 0.0 11.613235 8 GTCCTAG 85 3.7549242E-5 11.563387 1 TAGAAAT 240 0.0 11.434017 4 CACCTTT 280 0.0 11.152391 14 TAAGATA 60 0.0060453583 11.0397415 4 CTATGCA 95 1.417955E-5 10.956737 9 AACCATC 140 8.8402885E-9 10.814441 7 CTAGAAC 105 3.6245783E-6 10.81444 3 GTCCTAC 550 0.0 10.722413 1 GTATCAA 1035 0.0 10.636082 1 >>END_MODULE