FastQCFastQC Report
Thu 26 May 2016
SRR1512060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732681
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56710.7740066959563575No Hit
TATCAACGCAGAGTACTTTTTTTTT44220.6035368734824569No Hit
GGTATCAACGCAGAGTACTTTTTTT41850.5711899175766807No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19850.27092281634162757No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14680.20036004755139003No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13810.18848584854800385No Hit
CTGTAGGACGTGGAATATGGCAAGA10300.14057959739641127No Hit
GTCCTAAAGTGTGTATTTCTCATTT9250.12624866756473827No Hit
CTTTAGGACGTGAAATATGGCGAGG8430.11505689379143175No Hit
GTCCTACAGTGGACATTTCTAAATT7950.10850561158266694No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7610.10386512001812523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.013.6234141
CGAAATC852.7038732E-713.40709113
AAGTCGT1001.0088115E-813.2953667
TAGGACC1800.013.1979534
AGTCGTC1051.9965228E-812.6622538
CAAGTCG1103.819514E-812.0866976
ATAGCCC802.8537355E-511.8781593
AATGTCC2000.011.8708638
ACGAAAT1052.7243914E-711.75780712
ATAGACT1302.59206E-911.6954184
CTACACT752.0658517E-411.4030324
GACGAAA1001.9328945E-611.396028511
ACACTGT2350.011.3152056
ATACCCA855.299347E-511.1794434
ATAAGGT600.00585902211.0862814
CCACCTT2400.011.07947313
ATGTCCA2500.011.0161619
TGCTAGA700.001486469610.860032
AAATGTC2100.010.853367
TGACGAA1158.8300476E-710.73538910