##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512060_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732681 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.76074853858637 31.0 28.0 31.0 16.0 34.0 2 27.949935920270896 31.0 28.0 31.0 16.0 34.0 3 27.895223159874487 31.0 27.0 31.0 16.0 34.0 4 31.33056951115151 35.0 32.0 37.0 19.0 37.0 5 30.69067438626087 35.0 30.0 37.0 16.0 37.0 6 30.69259063630693 35.0 31.0 37.0 11.0 37.0 7 30.396303439013703 35.0 30.0 37.0 10.0 37.0 8 30.210454481554727 35.0 30.0 37.0 10.0 37.0 9 31.195749582696973 35.0 30.0 39.0 10.0 39.0 10 30.817752882905385 35.0 30.0 39.0 8.0 39.0 11 30.902735296807204 35.0 30.0 39.0 2.0 39.0 12 30.55224033378783 35.0 30.0 39.0 2.0 39.0 13 30.533638786866316 35.0 30.0 39.0 2.0 39.0 14 30.938902469150968 36.0 29.0 39.0 2.0 41.0 15 30.82156354539015 36.0 29.0 39.0 2.0 41.0 16 30.6318288586711 36.0 28.0 39.0 2.0 40.0 17 30.565155913692315 36.0 28.0 39.0 2.0 40.0 18 30.408382365586114 36.0 27.0 39.0 2.0 40.0 19 30.434086594302297 36.0 27.0 39.0 2.0 40.0 20 30.256924910022235 36.0 27.0 39.0 2.0 40.0 21 30.040450073087744 36.0 27.0 39.0 2.0 40.0 22 29.83131130737661 36.0 27.0 39.0 2.0 40.0 23 29.775632232854406 36.0 27.0 39.0 2.0 40.0 24 29.481171205476873 36.0 27.0 39.0 2.0 40.0 25 28.897771335683604 36.0 26.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33292867210576915 1101 2 0.328786192580548 1101 3 0.37961281668164304 1101 4 0.5154421350282874 1101 5 0.3141400619155128 1101 6 0.001959888223371564 1101 7 -0.34873034543671366 1101 8 -0.14745366210051358 1101 9 0.07053172527514917 1101 10 0.16205603033067462 1101 11 0.015300933900036284 1101 12 -0.04383766894033769 1101 13 0.06099898623781996 1101 14 0.044085325388046215 1101 15 -0.05692895581406532 1101 16 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-0.7448827166664422 2315 11 -1.1250690756859072 2315 12 -1.0438784188947352 2315 13 -1.2486036434925722 2315 14 -0.9666707220992343 2315 15 -1.125225879386555 2315 16 -1.3092363973658365 2315 17 -1.2041626225030306 2315 18 -1.506685767057423 2315 19 -1.4329752043145092 2315 20 -1.33609790769896 2315 21 -1.3147042365070263 2315 22 -1.331674497067322 2315 23 -1.6585333397449205 2315 24 -1.185272364973656 2315 25 -1.3540133950240474 2316 1 -1.5336603209793864 2316 2 -1.5150065515702522 2316 3 -1.4732090077002837 2316 4 -1.780912890073541 2316 5 -1.768057532097938 2316 6 -1.8865178990267921 2316 7 -2.1090884017026994 2316 8 -2.5350088387605503 2316 9 -2.713486461709792 2316 10 -2.702394097546563 2316 11 -2.855760920793532 2316 12 -2.5301595406842026 2316 13 -2.2724669605273604 2316 14 -2.6636706658973566 2316 15 -2.4049785911271258 2316 16 -2.8188030808930655 2316 17 -2.736527035474424 2316 18 -2.3419018632750372 2316 19 -2.003395032336762 2316 20 -2.0439425657105375 2316 21 -1.8508971213493801 2316 22 -2.5066286925374506 2316 23 -2.662869876585251 2316 24 -2.385180193895142 2316 25 -2.1010285133289983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45678.0 3 4.0 4 698.0 5 5191.0 6 5991.0 7 4920.0 8 4031.0 9 3575.0 10 3259.0 11 3145.0 12 2979.0 13 2861.0 14 2801.0 15 2864.0 16 2895.0 17 3039.0 18 3154.0 19 3573.0 20 4483.0 21 5743.0 22 7185.0 23 8640.0 24 10241.0 25 11739.0 26 13321.0 27 14948.0 28 17392.0 29 20490.0 30 25344.0 31 30850.0 32 37646.0 33 46338.0 34 56863.0 35 69582.0 36 84231.0 37 96931.0 38 70056.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49571722450948 24.201037069907716 16.458759624945557 18.844486080637253 2 18.33212544067609 25.501821392939085 37.57337777286431 18.59267539352051 3 21.175245434233997 28.576829479678057 31.21508541916605 19.032839666921895 4 17.100757355520344 21.781512008636774 36.189555891308764 24.928174744534115 5 13.3087375883426 40.367243055479705 34.9259123728795 11.398106983298197 6 29.90482897741309 40.59774990753138 17.47336153114384 12.02405958391169 7 26.54593199496097 34.48649548712196 22.934264707287348 16.03330781062973 8 25.514769510467367 38.24376448493022 20.49141618599674 15.75004981860568 9 27.278993177112547 16.13635402037176 21.10359078507563 35.48106201744006 10 16.255505465543667 31.06263162276625 33.4991626642427 19.182700247447386 11 33.96089157491459 23.736796777861034 24.922169402509414 17.380142244714957 12 23.58284164595506 27.130497447047215 31.350478584813857 17.936182322183868 13 29.48459152073003 23.55568112179789 26.41149422463528 20.548233132836803 14 21.634927069215664 24.02423428477059 28.49575736234459 25.845081283669153 15 23.43107027478534 32.46433304534989 24.180373177412818 19.924223502451955 16 21.964129000206093 29.989859161081018 27.989670811717517 20.056341026995376 17 21.55972380886088 30.191993514230614 28.273423222384643 19.974859454523866 18 21.24498929274814 28.376605917172686 31.00025795673697 19.378146833342207 19 23.26742470461224 27.91856210274321 29.828533836690184 18.985479355954364 20 23.360098050857058 27.980799283726476 30.045545059855517 18.613557605560946 21 22.98039665284073 27.40483238953924 29.433819083612107 20.180951874007924 22 23.233712898246303 28.32815372583703 29.70733511582803 18.730798260088633 23 22.35188301593736 28.457814519552166 29.924209853947353 19.266092610563124 24 22.7906240317537 27.828985352950085 30.239585935228675 19.140804680067536 25 22.526222404203775 28.223018391510564 30.158665165318865 19.0920940389668 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 720.0 1 720.0 2 722.0 3 724.0 4 724.0 5 724.0 6 1463.0 7 2202.0 8 2202.0 9 2202.0 10 2925.5 11 3649.0 12 3649.0 13 3649.0 14 4814.0 15 5979.0 16 5979.0 17 5979.0 18 9940.0 19 13901.0 20 13901.0 21 13901.0 22 20804.5 23 27708.0 24 27708.0 25 27708.0 26 38471.5 27 49235.0 28 49235.0 29 49235.0 30 59328.0 31 69421.0 32 69421.0 33 69421.0 34 75582.0 35 81743.0 36 81743.0 37 81743.0 38 83340.5 39 84938.0 40 84938.0 41 84938.0 42 86605.5 43 88273.0 44 88273.0 45 88273.0 46 86865.5 47 85458.0 48 85458.0 49 85458.0 50 81414.0 51 77370.0 52 77370.0 53 77370.0 54 67407.5 55 57445.0 56 57445.0 57 57445.0 58 47938.5 59 38432.0 60 38432.0 61 38432.0 62 30850.0 63 23268.0 64 23268.0 65 23268.0 66 18005.5 67 12743.0 68 12743.0 69 12743.0 70 9358.5 71 5974.0 72 5974.0 73 5974.0 74 4131.5 75 2289.0 76 2289.0 77 2289.0 78 1571.5 79 854.0 80 854.0 81 854.0 82 556.0 83 258.0 84 258.0 85 258.0 86 163.0 87 68.0 88 68.0 89 68.0 90 45.5 91 23.0 92 23.0 93 23.0 94 12.5 95 2.0 96 2.0 97 2.0 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34762741220258203 2 0.0 3 0.0 4 0.0 5 0.041627939034859646 6 0.0 7 0.0 8 0.003139156058366465 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005732371932669197 25 8.189102760955996E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732681.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.39217188190584 #Duplication Level Percentage of deduplicated Percentage of total 1 90.67158371292398 71.98613958941074 2 6.696725388592976 10.633351461941926 3 1.3540653909654388 3.2250657677660444 4 0.47945031826995027 1.522584083076894 5 0.24018674265945975 0.9534473578487456 6 0.1260355948144592 0.600374376404867 7 0.0832911427156323 0.46288653031038807 8 0.05457035689223436 0.3465967323236172 9 0.045099774935313953 0.3222512175149743 >10 0.21478666700916987 3.0238365815786166 >50 0.016673040622741324 0.9435110214421384 >100 0.014609891341889539 2.287344496859181 >500 0.0017188107392975842 0.9440310938804202 >1k 0.0010312864435785504 1.9747143624665453 >5k 1.7188107392975842E-4 0.7738653271748862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5671 0.7740066959563575 No Hit TATCAACGCAGAGTACTTTTTTTTT 4422 0.6035368734824569 No Hit GGTATCAACGCAGAGTACTTTTTTT 4185 0.5711899175766807 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1985 0.27092281634162757 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1468 0.20036004755139003 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1381 0.18848584854800385 No Hit CTGTAGGACGTGGAATATGGCAAGA 1030 0.14057959739641127 No Hit GTCCTAAAGTGTGTATTTCTCATTT 925 0.12624866756473827 No Hit CTTTAGGACGTGAAATATGGCGAGG 843 0.11505689379143175 No Hit GTCCTACAGTGGACATTTCTAAATT 795 0.10850561158266694 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 761 0.10386512001812523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3648504601593327E-4 0.0 10 0.0 0.0 0.0 1.3648504601593327E-4 0.0 11 0.0 0.0 0.0 1.3648504601593327E-4 0.0 12 0.0 0.0 0.0 1.3648504601593327E-4 0.0 13 0.0 0.0 0.0 1.3648504601593327E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1155 0.0 13.623414 1 CGAAATC 85 2.7038732E-7 13.407091 13 AAGTCGT 100 1.0088115E-8 13.295366 7 TAGGACC 180 0.0 13.197953 4 AGTCGTC 105 1.9965228E-8 12.662253 8 CAAGTCG 110 3.819514E-8 12.086697 6 ATAGCCC 80 2.8537355E-5 11.878159 3 AATGTCC 200 0.0 11.870863 8 ACGAAAT 105 2.7243914E-7 11.757807 12 ATAGACT 130 2.59206E-9 11.695418 4 CTACACT 75 2.0658517E-4 11.403032 4 GACGAAA 100 1.9328945E-6 11.3960285 11 ACACTGT 235 0.0 11.315205 6 ATACCCA 85 5.299347E-5 11.179443 4 ATAAGGT 60 0.005859022 11.086281 4 CCACCTT 240 0.0 11.079473 13 ATGTCCA 250 0.0 11.016161 9 TGCTAGA 70 0.0014864696 10.86003 2 AAATGTC 210 0.0 10.85336 7 TGACGAA 115 8.8300476E-7 10.735389 10 >>END_MODULE