FastQCFastQC Report
Thu 26 May 2016
SRR1512059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424585
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39940.16472922170185827No Hit
GTCCTACAGTGGACATTTCTAAATT29780.12282514327194138No Hit
CTGTAGGACGTGGAATATGGCAAGA28640.11812330770008064No Hit
GTCCTAAAGTGTGTATTTCTCATTT28580.1178758426699827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1307.2759576E-1213.5960571
ACCGTCC500.001543757713.2493028
CCCACGA450.00924992413.09252
TAGGACC8350.012.5806044
GGTATCA11350.012.2850341
GTCAACG1301.5552359E-912.0853842
TGGTATC4250.011.7832512
TAGAACG658.2875276E-411.64773754
GTCCTAC19750.010.8386011
GCCGGTT1159.267005E-710.698194511
GTGGTAT5050.010.6943691
TGGCGAG18500.010.64036618
GGCGAGG6950.010.62122919
ATGTCGA909.894914E-510.51531917
TCCAACG1002.5031763E-510.41016618
GTAGGAC22400.010.3510173
CCGGTTT1106.3305215E-610.32413112
CTACACT4050.010.2816464
TCCTACA21700.010.2718812
GACCTAC2200.010.265711
Produced by FastQC (version 0.11.5)