FastQCFastQC Report
Thu 26 May 2016
SRR1512059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424585
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47050.19405382776846347No Hit
GTCCTACAGTGGACATTTCTAAATT34040.14039516040889471No Hit
CTGTAGGACGTGGAATATGGCAAGA32630.13457973220159328No Hit
TATCAACGCAGAGTACTTTTTTTTT30460.12562974694638465No Hit
GTCCTAAAGTGTGTATTTCTCATTT29420.12134035309135378No Hit
CTTTAGGACGTGAAATATGGCGAGG28340.11688598254959097No Hit
GGTATCAACGCAGAGTACTTTTTTT28330.11684473837790797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.012.4774021
GACCGTG701.0968526E-412.207757
TAGGACC7250.012.192514
TGCACCG657.9915236E-411.6983555
CTGATCG658.0623827E-411.6860519
CCAACGA1157.118251E-811.56069819
AGGACCT13000.010.8209785
GTATAGG2300.010.7885611
ACCGTAT803.7910198E-410.681788
GGACCTG12300.010.5824046
TGGCCCG909.469427E-510.5610145
GGCGAGG8250.010.47469219
TGTAGGA21850.010.1356742
GTAGGAC21550.010.1003483
TGGCGAG19150.010.0657318
TCGAACT1701.5716068E-910.05489319
AGGACGT20800.09.9618815
GCCGGTT1054.131006E-59.94705611
CTGTAGG21850.09.9149921
CCGTTTC1252.588864E-69.87471413
Produced by FastQC (version 0.11.5)