FastQCFastQC Report
Thu 26 May 2016
SRR1512058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159012
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39570.18327827728609197No Hit
GTCCTACAGTGGACATTTCTAAATT36670.16984620743191792No Hit
CTGTAGGACGTGGAATATGGCAAGA35090.16252804523550587No Hit
GTCCTAAAGTGTGTATTTCTCATTT31750.14705800616207784No Hit
CTTTAGGACGTGAAATATGGCGAGG30190.13983247893017733No Hit
TATCAACGCAGAGTACTTTTTTTTT22380.10365852528841896No Hit
GGTATCAACGCAGAGTACTTTTTTT22300.10328798543037278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT400.005410735514.1975466
TCCAACG1700.013.36301418
TAGGACC7400.013.1742984
GTCCGGA450.009327820513.0757851
CGTGCGC701.1294937E-412.16932410
GGCGAGT550.0031539512.0469619
GGTATCA9900.011.9861361
TGTCGAG802.9764242E-511.83183618
ACACCGT855.5286328E-511.1353296
CGGTTTC1701.2732926E-1111.13532913
GCCGGTT1451.2951205E-911.09693211
CCGGTTT1451.2951205E-911.09693212
CGTTAGG802.8108002E-411.0326941
GACGTGG9600.010.943947
GTAGGAC21250.010.8680823
GTATCAA20450.010.8378741
AGGACCT13700.010.70861155
ACCGTGG803.9026787E-410.6481598
CCAACGT1252.3563734E-710.60132419
TGTAGGA21200.010.5932372
Produced by FastQC (version 0.11.5)