##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512058_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2159012 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.690352809525837 31.0 30.0 34.0 25.0 34.0 2 26.707907135300776 31.0 25.0 34.0 10.0 34.0 3 28.701656127895536 31.0 28.0 34.0 16.0 34.0 4 33.24180782691342 35.0 33.0 37.0 28.0 37.0 5 33.7128950649649 35.0 35.0 37.0 28.0 37.0 6 34.00837281126738 37.0 35.0 37.0 30.0 37.0 7 34.084095410308045 37.0 35.0 37.0 30.0 37.0 8 34.12665144982983 37.0 35.0 37.0 30.0 37.0 9 35.774797453650095 39.0 37.0 39.0 30.0 39.0 10 35.68320555883895 39.0 37.0 39.0 30.0 39.0 11 35.69571405809695 39.0 37.0 39.0 30.0 39.0 12 35.603884091427005 39.0 37.0 39.0 30.0 39.0 13 35.60304157642477 39.0 37.0 39.0 30.0 39.0 14 36.828825407176986 40.0 37.0 41.0 31.0 41.0 15 36.77702625089624 40.0 37.0 41.0 30.0 41.0 16 36.72833036592664 40.0 37.0 41.0 30.0 41.0 17 36.701527365294865 40.0 37.0 41.0 30.0 41.0 18 36.649397502190816 40.0 37.0 41.0 30.0 41.0 19 36.691818757839236 40.0 37.0 41.0 30.0 41.0 20 36.59661873116036 40.0 37.0 41.0 30.0 41.0 21 36.54176401057521 40.0 37.0 41.0 30.0 41.0 22 36.44900398886157 40.0 37.0 41.0 30.0 41.0 23 36.3597872545405 40.0 37.0 41.0 30.0 41.0 24 36.28316794904336 40.0 37.0 41.0 30.0 41.0 25 36.188045272559854 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0139692645439844 1101 2 3.95595675344083 1101 3 2.063016278150247 1101 4 1.2404354768515446 1101 5 0.9243734118713149 1101 6 0.6197006415717965 1101 7 0.13933397015480864 1101 8 0.09802635062582254 1101 9 0.31699627757360105 1101 10 0.32447940882712345 1101 11 0.43103212071715546 1101 12 0.31695154166084905 1101 13 0.41463765202502145 1101 14 0.22638395306539394 1101 15 0.38539498149221885 1101 16 0.4491087588284799 1101 17 0.4197581904389409 1101 18 0.5280054515847894 1101 19 0.4105680596568462 1101 20 0.38645267237211556 1101 21 0.5182440339647911 1101 22 0.5422878650737744 1101 23 0.4767600041140909 1101 24 0.3747175994299994 1101 25 0.17949925306377423 1102 1 1.4995783622939456 1102 2 4.251774366976505 1102 3 2.4556890760730496 1102 4 1.6012502267943987 1102 5 0.9639551989073283 1102 6 0.9249265058503369 1102 7 0.6637610589724119 1102 8 0.7690221361363001 1102 9 0.7160485952860114 1102 10 0.5644302526239073 1102 11 0.7176194628664163 1102 12 0.7206855126037226 1102 13 0.6649947664480251 1102 14 0.8585671408220605 1102 15 0.7933783700856125 1102 16 0.8054455173441397 1102 17 0.8462686393424264 1102 18 0.8447465545431498 1102 19 0.895998921225889 1102 20 1.0447603018565914 1102 21 0.9049949733002833 1102 22 0.810829435966312 1102 23 0.8940525092056362 1102 24 0.7887926075841776 1102 25 0.8883396649074911 1103 1 1.2692318191327594 1103 2 4.037616405410414 1103 3 2.396650010375865 1103 4 1.5216838972043973 1103 5 1.0204859098097927 1103 6 0.9928254248888848 1103 7 0.7649898907106163 1103 8 0.6799765300226852 1103 9 0.697771531376624 1103 10 0.6641761778455617 1103 11 0.6849970031505634 1103 12 0.6687508969861042 1103 13 0.6619438909100523 1103 14 0.7190577091459218 1103 15 0.7894407673893298 1103 16 0.683766076877383 1103 17 0.7498060278987992 1103 18 0.5517240290522167 1103 19 0.6153564520394141 1103 20 0.7701594959882669 1103 21 0.9341113247122834 1103 22 0.8400343117293119 1103 23 0.8781290061140794 1103 24 0.9479996964559447 1103 25 0.9374288902823267 1104 1 1.108883142671047 1104 2 3.679753001438929 1104 3 1.9635432981417367 1104 4 1.3387076894388983 1104 5 0.8974032046745322 1104 6 0.671297231273428 1104 7 0.4417477900763487 1104 8 0.43012647005858895 1104 9 0.3931370190078525 1104 10 0.36649363673218005 1104 11 0.3396667976319563 1104 12 0.47426668713253406 1104 13 0.4650842493305305 1104 14 0.5252779491787507 1104 15 0.4629423149612819 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-0.6112607778490684 2315 13 -0.800169464748052 2315 14 -0.8565011399543891 2315 15 -1.2346535213760248 2315 16 -1.0803759411400549 2315 17 -0.9710393038398024 2315 18 -0.8958313033141039 2315 19 -0.8348375584619276 2315 20 -0.8953015979806267 2315 21 -0.8694041312885119 2315 22 -1.2706512822532972 2315 23 -1.0590214019225357 2315 24 -0.9360493814538415 2315 25 -0.9516208961614083 2316 1 -0.4694624503086935 2316 2 0.15098132144773757 2316 3 0.13917922499717506 2316 4 -0.2635493128181068 2316 5 -0.6284521504491138 2316 6 -0.8133974778115203 2316 7 -0.8507528438876975 2316 8 -1.0558462744507366 2316 9 -1.0230005922517122 2316 10 -1.1604049389468827 2316 11 -1.2377173358497373 2316 12 -1.0666940684623683 2316 13 -1.22973276743771 2316 14 -1.3431134606760722 2316 15 -1.0504557594124009 2316 16 -1.3752331799089745 2316 17 -1.4708344752912552 2316 18 -1.4818777851704183 2316 19 -1.266129528756096 2316 20 -1.2020841255364658 2316 21 -1.209067899536386 2316 22 -1.5475784841957605 2316 23 -1.4093147761457132 2316 24 -1.4132192483295114 2316 25 -1.2479780773223013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63231.0 3 5.0 4 528.0 5 2458.0 6 3020.0 7 2737.0 8 2578.0 9 2508.0 10 2481.0 11 2454.0 12 2342.0 13 2313.0 14 2248.0 15 2491.0 16 2521.0 17 2850.0 18 3200.0 19 4158.0 20 5215.0 21 6565.0 22 8293.0 23 9707.0 24 11343.0 25 13173.0 26 15272.0 27 17392.0 28 20380.0 29 24706.0 30 32010.0 31 41129.0 32 53953.0 33 72794.0 34 102421.0 35 152924.0 36 250597.0 37 348611.0 38 868404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.797220209984935 19.57650073274257 13.497284869190166 25.128994188082327 2 16.456007225351694 21.792334485409643 40.10650867128585 21.64514961795281 3 18.09948254108824 25.231680046243376 30.263842905921784 26.404994506746604 4 11.725687490389122 17.59930005020815 39.73247948598711 30.942532973415616 5 12.661346949437984 38.10733798607882 36.562974175224596 12.668340889258605 6 30.30756240478849 38.41141931321268 18.92484005393215 12.356178228066673 7 27.02013698858552 32.023351421854066 23.30737392844505 17.649137661115365 8 23.990243681018764 38.10207544102369 21.269275608137246 16.638405269820304 9 25.776744177429308 15.95563155739755 21.142633760257006 37.12499050491614 10 14.107768888411304 29.81363208768467 35.513608326783256 20.564990697120763 11 33.278246382255574 23.02938706657817 25.546187583110967 18.14617896805528 12 22.634241958821903 25.176840147252538 32.87207296670885 19.31684492721671 13 28.172284359697862 22.66291248033823 26.476184476973724 22.68861868299018 14 20.10860528269657 22.929897068611506 28.644041183671597 28.317456465020324 15 22.101683547845035 31.93340287131336 24.024692776140196 21.94022080470141 16 21.182559429961483 28.985619348109225 28.8016463085893 21.03017491333999 17 20.869360614947947 29.27996694784466 28.685528380574077 21.165144056633313 18 20.548485155866068 28.395077167634476 30.81161148142127 20.244826195078186 19 22.665880498046775 27.68627425550974 28.35288101773326 21.29496422871022 20 22.682245505667677 28.095129358679056 29.08934102270638 20.133284112946892 21 22.292055745772007 27.55958468584641 27.92179880534187 22.226560763039707 22 22.190350160886233 28.825014045991182 28.326815591999736 20.657820201122846 23 21.53074212410236 28.48037417461482 28.5391516040819 21.449732097200915 24 22.128269782659846 27.641856552904752 29.420262601597397 20.809611062838 25 22.035330229119857 28.012678085836924 28.776827801376655 21.175163883666567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 407.0 3 650.0 4 650.0 5 650.0 6 1465.5 7 2281.0 8 2281.0 9 2281.0 10 3674.0 11 5067.0 12 5067.0 13 5067.0 14 8903.5 15 12740.0 16 12740.0 17 12740.0 18 22021.5 19 31303.0 20 31303.0 21 31303.0 22 48884.5 23 66466.0 24 66466.0 25 66466.0 26 92742.5 27 119019.0 28 119019.0 29 119019.0 30 152402.0 31 185785.0 32 185785.0 33 185785.0 34 208890.0 35 231995.0 36 231995.0 37 231995.0 38 246437.0 39 260879.0 40 260879.0 41 260879.0 42 269279.5 43 277680.0 44 277680.0 45 277680.0 46 277551.5 47 277423.0 48 277423.0 49 277423.0 50 262834.5 51 248246.0 52 248246.0 53 248246.0 54 215088.0 55 181930.0 56 181930.0 57 181930.0 58 152531.0 59 123132.0 60 123132.0 61 123132.0 62 97842.0 63 72552.0 64 72552.0 65 72552.0 66 54828.5 67 37105.0 68 37105.0 69 37105.0 70 26519.0 71 15933.0 72 15933.0 73 15933.0 74 10845.5 75 5758.0 76 5758.0 77 5758.0 78 3864.0 79 1970.0 80 1970.0 81 1970.0 82 1300.5 83 631.0 84 631.0 85 631.0 86 412.0 87 193.0 88 193.0 89 193.0 90 128.5 91 64.0 92 64.0 93 64.0 94 48.5 95 33.0 96 33.0 97 33.0 98 23.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.4848347299598146 3 0.0 4 0.0 5 0.0 6 1.3895244676731763E-4 7 0.0 8 4.6317482255772545E-5 9 0.0 10 1.3895244676731763E-4 11 4.6317482255772545E-5 12 0.0 13 0.0 14 4.6317482255772545E-5 15 0.0 16 0.0 17 0.0 18 5.094923048134981E-4 19 8.33714680603906E-4 20 6.947622338365882E-4 21 0.003149588793392533 22 0.001528476914440494 23 9.263496451154509E-4 24 0.0 25 5.094923048134981E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2159012.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.16453321617709 #Duplication Level Percentage of deduplicated Percentage of total 1 85.69554975307442 65.26961545646593 2 10.296262885480086 15.684201130872788 3 2.2636453659339937 5.17228477989975 4 0.750402594469161 2.286162533278075 5 0.3341468980524114 1.2725071257897713 6 0.1758860702404663 0.8037768263455711 7 0.10548079353587961 0.5623726782052555 8 0.07259113776412575 0.4423096098748674 9 0.0490695380836033 0.3363622616943963 >10 0.2273350565785186 3.0301210765643494 >50 0.015199512956566507 0.7999470545608469 >100 0.011730689686034183 1.8151647201767342 >500 0.0015339228094517316 0.8024854398627028 >1k 0.0011657813351830747 1.7226893064089266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3957 0.18327827728609197 No Hit GTCCTACAGTGGACATTTCTAAATT 3667 0.16984620743191792 No Hit CTGTAGGACGTGGAATATGGCAAGA 3509 0.16252804523550587 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3175 0.14705800616207784 No Hit CTTTAGGACGTGAAATATGGCGAGG 3019 0.13983247893017733 No Hit TATCAACGCAGAGTACTTTTTTTTT 2238 0.10365852528841896 No Hit GGTATCAACGCAGAGTACTTTTTTT 2230 0.10328798543037278 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.6317482255772545E-5 5 0.0 0.0 0.0 0.0 4.6317482255772545E-5 6 0.0 0.0 0.0 0.0 4.6317482255772545E-5 7 0.0 0.0 0.0 0.0 4.6317482255772545E-5 8 0.0 0.0 0.0 0.0 4.6317482255772545E-5 9 0.0 0.0 0.0 0.0 4.6317482255772545E-5 10 0.0 0.0 0.0 0.0 4.6317482255772545E-5 11 0.0 0.0 0.0 0.0 4.6317482255772545E-5 12 0.0 0.0 0.0 0.0 9.263496451154509E-5 13 0.0 0.0 0.0 0.0 9.263496451154509E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 40 0.0054107355 14.197546 6 TCCAACG 170 0.0 13.363014 18 TAGGACC 740 0.0 13.174298 4 GTCCGGA 45 0.0093278205 13.075785 1 CGTGCGC 70 1.1294937E-4 12.169324 10 GGCGAGT 55 0.00315395 12.04696 19 GGTATCA 990 0.0 11.986136 1 TGTCGAG 80 2.9764242E-5 11.831836 18 ACACCGT 85 5.5286328E-5 11.135329 6 CGGTTTC 170 1.2732926E-11 11.135329 13 GCCGGTT 145 1.2951205E-9 11.096932 11 CCGGTTT 145 1.2951205E-9 11.096932 12 CGTTAGG 80 2.8108002E-4 11.032694 1 GACGTGG 960 0.0 10.94394 7 GTAGGAC 2125 0.0 10.868082 3 GTATCAA 2045 0.0 10.837874 1 AGGACCT 1370 0.0 10.7086115 5 ACCGTGG 80 3.9026787E-4 10.648159 8 CCAACGT 125 2.3563734E-7 10.601324 19 TGTAGGA 2120 0.0 10.593237 2 >>END_MODULE