FastQCFastQC Report
Thu 26 May 2016
SRR1512058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159012
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50220.23260639588848975No Hit
GTCCTACAGTGGACATTTCTAAATT39950.18503834161181132No Hit
CTGTAGGACGTGGAATATGGCAAGA39180.18147189547811685No Hit
GTCCTAAAGTGTGTATTTCTCATTT34610.16030480608722877No Hit
CTTTAGGACGTGAAATATGGCGAGG34600.16025848860497302No Hit
TATCAACGCAGAGTACTTTTTTTTT32130.1488180704877972No Hit
GGTATCAACGCAGAGTACTTTTTTT29980.1388598118028061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1550.013.47655718
TATACCG500.001492146413.3091235
GCCGGTT1352.7284841E-1112.65891611
TAGGACC5300.012.5557764
GGTATCA10500.012.2738951
CCAACGA951.0463446E-611.99432519
TAGGACT4000.011.17015654
GGCGAGG10050.011.14895319
TGCCGGT1553.1650416E-1011.02550810
TGAACCG700.001483300510.864595
GGACGTG22750.010.7335046
AGGACGT23000.010.7051655
GACGTGG10050.010.6750577
GTAGGAC21050.010.5678143
GTATCAA22600.010.5601361
TGTAGGA21700.010.5579282
CGGTTTC1553.7016434E-910.41297913
GTCCTAC20550.010.1734961
TAGGACG25300.010.1452934
TTAGGAC14550.010.1272163
Produced by FastQC (version 0.11.5)