##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512058_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2159012 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.328022725209493 33.0 31.0 34.0 28.0 34.0 2 31.523284724679623 33.0 31.0 34.0 28.0 34.0 3 31.640916308014962 34.0 31.0 34.0 28.0 34.0 4 35.164013910066274 37.0 35.0 37.0 32.0 37.0 5 34.86514433453821 37.0 35.0 37.0 32.0 37.0 6 34.8187666395555 37.0 35.0 37.0 32.0 37.0 7 34.705795984459556 37.0 35.0 37.0 32.0 37.0 8 34.62965004363107 37.0 35.0 37.0 32.0 37.0 9 36.206791347153235 39.0 37.0 39.0 32.0 39.0 10 36.035300869101235 39.0 37.0 39.0 31.0 39.0 11 36.0337663709141 39.0 37.0 39.0 31.0 39.0 12 35.89077040794586 39.0 37.0 39.0 30.0 39.0 13 35.87926514535352 39.0 37.0 39.0 30.0 39.0 14 36.99365821032954 40.0 37.0 41.0 31.0 41.0 15 36.94095910536857 40.0 37.0 41.0 31.0 41.0 16 36.854443143437834 40.0 37.0 41.0 31.0 41.0 17 36.781569995905535 40.0 37.0 41.0 31.0 41.0 18 36.710757513158796 40.0 37.0 41.0 30.0 41.0 19 36.742261275064706 40.0 37.0 41.0 30.0 41.0 20 36.63489410897207 40.0 37.0 41.0 30.0 41.0 21 36.535798318860664 40.0 37.0 41.0 30.0 41.0 22 36.4250036590811 40.0 36.0 41.0 30.0 41.0 23 36.52844773442667 40.0 37.0 41.0 30.0 41.0 24 36.45166029646894 40.0 37.0 41.0 30.0 41.0 25 36.010150476236355 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2279843734658371 1101 2 0.2352616910460128 1101 3 0.2137468101577653 1101 4 0.12030545755308708 1101 5 0.13486066411240927 1101 6 0.26790053654259793 1101 7 0.07021901761018512 1101 8 0.33871203602128475 1101 9 0.2683823908099896 1101 10 0.20218280471213035 1101 11 0.08683484607918501 1101 12 0.21976904150598386 1101 13 0.11252718153339458 1101 14 -0.010218032800125343 1101 15 -0.023440359571957003 1101 16 0.18363774234185115 1101 17 0.2115726128861155 1101 18 0.19155151467431608 1101 19 0.2131756176659465 1101 20 0.20439716732119706 1101 21 0.27609520323400716 1101 22 0.05690129003102129 1101 23 -0.05719784276134021 1101 24 -0.02726266437272784 1101 25 0.17429276734496568 1102 1 0.16015985648845543 1102 2 0.05939754091670224 1102 3 0.04586193943036321 1102 4 0.10663700253203956 1102 5 0.26748815819902916 1102 6 0.2928964915572223 1102 7 0.296875342811596 1102 8 0.17513436844973995 1102 9 0.32072296060075445 1102 10 0.21748004051038095 1102 11 0.3153944974142888 1102 12 0.3941102225389841 1102 13 0.4466509349716361 1102 14 0.5753230064657728 1102 15 0.5382231952382313 1102 16 0.4093294348407781 1102 17 0.48352738096077985 1102 18 0.5182728164661796 1102 19 0.43682170327581815 1102 20 0.7045987745287192 1102 21 0.597039752263079 1102 22 0.6802789940716423 1102 23 0.4777901250123264 1102 24 0.5430777050949516 1102 25 0.5362614296233588 1103 1 0.25216682469016405 1103 2 0.18754370995122827 1103 3 0.23437553397647193 1103 4 0.2793079528211635 1103 5 0.31995293082373877 1103 6 0.3440608464112884 1103 7 0.22065810207605807 1103 8 0.32574297336284275 1103 9 0.28704686328826057 1103 10 0.33266357399200075 1103 11 0.5366200651261437 1103 12 0.6008123566434875 1103 13 0.601040370406352 1103 14 0.5947644870479039 1103 15 0.568702277651191 1103 16 0.6453511801799579 1103 17 0.587159475736236 1103 18 0.5313986533365664 1103 19 0.660427784750361 1103 20 0.6064937643719048 1103 21 0.7455653149058037 1103 22 0.8186126344377556 1103 23 0.7104266793874174 1103 24 0.655205664818844 1103 25 0.8930093713703755 1104 1 0.013165149892753192 1104 2 -0.046148358644160226 1104 3 0.00109530862881968 1104 4 0.04027980749653892 1104 5 0.026778964372624614 1104 6 0.01482567533179946 1104 7 0.16339638988127092 1104 8 0.07362479462096161 1104 9 0.18041404074071465 1104 10 0.23688295218261857 1104 11 0.2275993148560147 1104 12 0.3238018664450948 1104 13 0.19540267275080225 1104 14 0.1318324063666907 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-0.4614086178263008 2314 17 -0.40385538068855453 2314 18 -0.42447075699011805 2314 19 -0.49759069340543505 2314 20 -0.465249795869191 2314 21 -0.5428483419667529 2314 22 -0.46161646382449817 2314 23 -0.3337974547803313 2314 24 -0.3333515048989284 2314 25 -0.33408177682592566 2315 1 -0.7718938228083729 2315 2 -0.6472605674136851 2315 3 -0.9039167601436766 2315 4 -0.6956268601766169 2315 5 -0.8291451637273894 2315 6 -0.6403972641974391 2315 7 -0.8685972531813633 2315 8 -0.7203295872788331 2315 9 -0.8996247675012796 2315 10 -0.9890576335001953 2315 11 -0.986185706737956 2315 12 -1.035501116815432 2315 13 -0.9845339912647049 2315 14 -1.1258671568673648 2315 15 -1.2396860876576383 2315 16 -1.2248833652287985 2315 17 -1.1323300565674614 2315 18 -1.0851970910243836 2315 19 -1.1081137216191124 2315 20 -1.066176145638643 2315 21 -1.3907778530791362 2315 22 -1.395346574304618 2315 23 -1.3056544099731795 2315 24 -1.2121154341191769 2315 25 -1.3160617049208696 2316 1 -0.9543643395879577 2316 2 -0.9338875390662693 2316 3 -0.9662372684598566 2316 4 -0.918409199972956 2316 5 -1.0315063817175982 2316 6 -1.1191418927821104 2316 7 -1.0964303236039754 2316 8 -1.1236402709367823 2316 9 -1.3947959344692649 2316 10 -1.2793735104153043 2316 11 -1.4265723490717477 2316 12 -1.3749324615575276 2316 13 -1.3802938980677197 2316 14 -1.7197086451743573 2316 15 -1.5487572897594006 2316 16 -1.5745083440702956 2316 17 -1.5362000359565897 2316 18 -1.43365412041301 2316 19 -1.461962293580072 2316 20 -1.4936298565203003 2316 21 -1.5876944722132507 2316 22 -1.5868720775925382 2316 23 -1.6871118788897377 2316 24 -1.751513209738043 2316 25 -1.652544229774115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25266.0 3 0.0 4 432.0 5 4029.0 6 5408.0 7 4202.0 8 3422.0 9 3126.0 10 3000.0 11 2845.0 12 2695.0 13 2736.0 14 2584.0 15 2467.0 16 2706.0 17 2906.0 18 3030.0 19 3415.0 20 4157.0 21 5406.0 22 6768.0 23 8470.0 24 10659.0 25 12826.0 26 14673.0 27 16704.0 28 19744.0 29 23976.0 30 30865.0 31 40174.0 32 52300.0 33 69788.0 34 93520.0 35 128250.0 36 196612.0 37 384568.0 38 965283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.95916420410917 20.18451729995114 14.064266933476045 24.792051562463644 2 14.249388146059403 21.119197114235586 41.88309282208714 22.748321917617872 3 18.1288478248384 25.541960859874795 30.571946797887183 25.757244517399624 4 12.083814263190757 18.13361852551074 39.402421107432474 30.38014610386603 5 12.579953404147663 38.253869550148 36.59373013328193 12.57244691242241 6 30.34528589382834 38.74678849084094 18.647792446604605 12.26013316872611 7 27.07557901484568 32.00343490448409 23.230162685524675 17.690823395145557 8 24.087916139419328 38.217434641400786 21.060281276806244 16.63436794237364 9 25.84279290712604 15.81593803091414 21.01442696937303 37.326842092586794 10 14.063006597462172 30.068197860873397 35.34176743806889 20.527028103595534 11 33.40782728396137 23.005754483995457 25.456366152666128 18.130052079377048 12 22.581532664014837 25.30393531856238 32.816491988001914 19.298040029420864 13 28.266864658464147 22.650453077611427 26.481418352468626 22.601263911455796 14 20.070569315964896 22.957908524825243 28.664453926147697 28.30706823306216 15 22.06217473548086 31.958877486554034 23.961932587683627 22.01701519028148 16 21.148377128056723 29.175104168017597 28.678117583413155 20.99840112051253 17 20.938142076097773 29.299096068016294 28.584139411916194 21.17862244396974 18 20.5652863439388 28.39622938640452 30.78093127782523 20.25755299183145 19 22.72636743102864 27.60489520206465 28.399378975197916 21.2693583917088 20 22.715760727592066 28.114294871913636 29.07227935740978 20.097665043084522 21 22.31076066274759 27.509388553653245 27.95700070217303 22.222850081426134 22 22.200386102532086 28.82837149585088 28.414154252037505 20.55708814957953 23 21.52947737205722 28.44796601408422 28.59970208595413 21.42285452790443 24 22.110402633892466 27.67500071794691 29.43281267514431 20.781783973016314 25 22.08263076951583 28.03206470381145 28.82571476217829 21.059589764494433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 163.0 2 356.5 3 550.0 4 550.0 5 550.0 6 1448.5 7 2347.0 8 2347.0 9 2347.0 10 3740.5 11 5134.0 12 5134.0 13 5134.0 14 8824.0 15 12514.0 16 12514.0 17 12514.0 18 22233.0 19 31952.0 20 31952.0 21 31952.0 22 49525.0 23 67098.0 24 67098.0 25 67098.0 26 94205.5 27 121313.0 28 121313.0 29 121313.0 30 154708.5 31 188104.0 32 188104.0 33 188104.0 34 209744.0 35 231384.0 36 231384.0 37 231384.0 38 246173.0 39 260962.0 40 260962.0 41 260962.0 42 269020.0 43 277078.0 44 277078.0 45 277078.0 46 277292.0 47 277506.0 48 277506.0 49 277506.0 50 262421.5 51 247337.0 52 247337.0 53 247337.0 54 214678.5 55 182020.0 56 182020.0 57 182020.0 58 151959.0 59 121898.0 60 121898.0 61 121898.0 62 96765.0 63 71632.0 64 71632.0 65 71632.0 66 54205.5 67 36779.0 68 36779.0 69 36779.0 70 26035.0 71 15291.0 72 15291.0 73 15291.0 74 10421.5 75 5552.0 76 5552.0 77 5552.0 78 3687.0 79 1822.0 80 1822.0 81 1822.0 82 1127.5 83 433.0 84 433.0 85 433.0 86 275.5 87 118.0 88 118.0 89 118.0 90 69.0 91 20.0 92 20.0 93 20.0 94 12.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47067825468316066 2 0.0 3 0.0 4 0.0 5 0.04075938438507984 6 0.05317246962962688 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.003612763615950259 25 0.011672005528454682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2159012.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.11741333973725 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75453308248923 62.81786760891289 2 10.747806126334911 15.9319917832185 3 2.549548014458762 5.668977120514395 4 0.8162363123105559 2.419892965696974 5 0.37831837251630057 1.4019989594903672 6 0.19354097459021877 0.86068538471273 7 0.12388751325058314 0.6427555419057952 8 0.07821572566195582 0.46377178148437387 9 0.05743746824169321 0.3831404917371846 >10 0.26762874651020263 3.442048768455884 >50 0.01648143769621729 0.8495474666021516 >100 0.013307815784664593 2.046528669522094 >500 0.001685246411781392 0.9152841796580236 >1k 0.0013107472091631776 1.923183923009383 >5k 6.241653376967511E-5 0.23232535507921492 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5022 0.23260639588848975 No Hit GTCCTACAGTGGACATTTCTAAATT 3995 0.18503834161181132 No Hit CTGTAGGACGTGGAATATGGCAAGA 3918 0.18147189547811685 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3461 0.16030480608722877 No Hit CTTTAGGACGTGAAATATGGCGAGG 3460 0.16025848860497302 No Hit TATCAACGCAGAGTACTTTTTTTTT 3213 0.1488180704877972 No Hit GGTATCAACGCAGAGTACTTTTTTT 2998 0.1388598118028061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.6317482255772545E-5 7 0.0 0.0 0.0 0.0 4.6317482255772545E-5 8 0.0 0.0 0.0 0.0 4.6317482255772545E-5 9 0.0 0.0 0.0 0.0 4.6317482255772545E-5 10 0.0 0.0 0.0 0.0 4.6317482255772545E-5 11 0.0 0.0 0.0 4.6317482255772545E-5 4.6317482255772545E-5 12 0.0 0.0 0.0 4.6317482255772545E-5 2.3158741127886275E-4 13 0.0 0.0 0.0 9.263496451154509E-5 2.7790489353463527E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 155 0.0 13.476557 18 TATACCG 50 0.0014921464 13.309123 5 GCCGGTT 135 2.7284841E-11 12.658916 11 TAGGACC 530 0.0 12.555776 4 GGTATCA 1050 0.0 12.273895 1 CCAACGA 95 1.0463446E-6 11.994325 19 TAGGACT 400 0.0 11.1701565 4 GGCGAGG 1005 0.0 11.148953 19 TGCCGGT 155 3.1650416E-10 11.025508 10 TGAACCG 70 0.0014833005 10.86459 5 GGACGTG 2275 0.0 10.733504 6 AGGACGT 2300 0.0 10.705165 5 GACGTGG 1005 0.0 10.675057 7 GTAGGAC 2105 0.0 10.567814 3 GTATCAA 2260 0.0 10.560136 1 TGTAGGA 2170 0.0 10.557928 2 CGGTTTC 155 3.7016434E-9 10.412979 13 GTCCTAC 2055 0.0 10.173496 1 TAGGACG 2530 0.0 10.145293 4 TTAGGAC 1455 0.0 10.127216 3 >>END_MODULE