FastQCFastQC Report
Thu 26 May 2016
SRR1512057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259840
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43210.19120822713112434No Hit
GTCCTACAGTGGACATTTCTAAATT33520.1483290852449731No Hit
CTGTAGGACGTGGAATATGGCAAGA31810.14076217785329934No Hit
GTCCTAAAGTGTGTATTTCTCATTT30180.13354927782497875No Hit
CTTTAGGACGTGAAATATGGCGAGG27810.12306180968564145No Hit
GGTATCAACGCAGAGTACTTTTTTT25610.11332660719342962No Hit
TATCAACGCAGAGTACTTTTTTTTT25360.112220334182951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC452.7228047E-517.413351
CGTTAGG705.296366E-613.992871
TAGGACC7000.012.3063954
GGTATCA10650.012.2322651
GGCGAGG8450.011.98711219
CTCGAAC1657.2759576E-1211.47449318
GCCGGTT752.1438798E-411.35974911
CCGGTTT752.1438798E-411.35974912
TCGAACT1751.8189894E-1211.35974919
CGGTAGG959.692058E-611.341591
TCCAACG1900.010.96116118
GTATCAA22450.010.9075821
GACCGTG1605.7843863E-1010.6497657
GCGTGCG803.8973984E-410.6497659
TTAGGAC13600.010.5105513
GTATTAG2250.010.4480091
TGGCGAG20250.010.4247918
GTCCTAG2950.010.2930591
AGGACCT14300.010.1946475
CCAACGA1502.5840563E-810.09755519
Produced by FastQC (version 0.11.5)