FastQCFastQC Report
Thu 26 May 2016
SRR1512057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259840
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52360.23169781931464176No Hit
GTCCTACAGTGGACATTTCTAAATT35520.15717926932880202No Hit
CTGTAGGACGTGGAATATGGCAAGA34570.1529754318889833No Hit
GGTATCAACGCAGAGTACTTTTTTT33320.1474440668365902No Hit
TATCAACGCAGAGTACTTTTTTTTT32100.14204545454545456No Hit
GTCCTAAAGTGTGTATTTCTCATTT31490.1393461483998867No Hit
CTTTAGGACGTGAAATATGGCGAGG31390.13890363919569526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTC508.755264E-515.1921427
AGCCCGT655.449378E-513.1551956
GGTATCA12000.012.8061131
TAGGACC7000.012.2198674
GGCGAAA550.003079055312.08572719
GTCGAGG1251.844819E-811.39511519
GGCGAGG9000.011.39511419
TTAGGAC12850.011.0205973
GTCCTAA10950.010.8088691
TGGTATC4500.010.7715862
TGTCGAG1701.4006218E-1010.61215718
GACGTGA11050.010.5691947
AGGACGG1201.5154674E-610.296375
AGGACGT20600.010.288685
TGTAGGA21000.010.273742
GGACGTG20050.010.2354636
TGGCGAG20800.010.22547918
GTGGTAT5150.010.1936141
TATGTCG1701.5734258E-910.05362216
TAGGACG22300.09.9731654
Produced by FastQC (version 0.11.5)