FastQCFastQC Report
Thu 26 May 2016
SRR1512056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44260.23452709354907425No Hit
GTCCTACAGTGGACATTTCTAAATT32980.17475606744799974No Hit
CTGTAGGACGTGGAATATGGCAAGA29480.15621009303720534No Hit
GTCCTAAAGTGTGTATTTCTCATTT28020.14847377228298825No Hit
TATCAACGCAGAGTACTTTTTTTTT25970.1376111301280944No Hit
CTTTAGGACGTGAAATATGGCGAGG25520.1352266477038494No Hit
GGTATCAACGCAGAGTACTTTTTTT24450.12955687838397797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG508.9821966E-515.1463764
CGTTAGG601.928232E-514.6897221
CGGTAGG851.8642822E-713.8256211
GACGTAA500.001540226513.2530787
GGTATCA8750.012.8709951
TAGGACC6500.012.6705264
GGACGTA604.216579E-412.621986
CTAGGAC3100.011.6040783
CCAACGA1751.8189894E-1211.36038319
GTCTTAA1654.5474735E-1111.2769581
CCGTGCT600.00603305411.0442329
TCCAACG2100.010.81941218
TATGTCG1053.6145102E-610.81941216
GCCGGTT1252.352208E-710.60246311
AATGTCC5100.010.5801898
GTCCTAA10200.010.5612381
GTAGGAC19900.010.465463
AGGACCT12750.010.3203255
GTATCAA18950.010.2840981
CTGTAGG20250.010.2042181
Produced by FastQC (version 0.11.5)