##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512056_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887202 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.035021158307377 31.0 30.0 34.0 25.0 34.0 2 27.08100033806662 31.0 26.0 34.0 10.0 34.0 3 29.06273467281192 31.0 28.0 34.0 16.0 34.0 4 33.614374613846316 35.0 33.0 37.0 28.0 37.0 5 34.08238810683753 37.0 35.0 37.0 30.0 37.0 6 34.37759603900378 37.0 35.0 37.0 32.0 37.0 7 34.47136395573977 37.0 35.0 37.0 32.0 37.0 8 34.513521075115435 37.0 35.0 37.0 32.0 37.0 9 36.20035375121476 39.0 37.0 39.0 32.0 39.0 10 36.115565795288475 39.0 37.0 39.0 32.0 39.0 11 36.13503429945496 39.0 37.0 39.0 32.0 39.0 12 36.049188163217295 39.0 37.0 39.0 32.0 39.0 13 36.05262075813824 39.0 37.0 39.0 32.0 39.0 14 37.30643831449945 40.0 38.0 41.0 32.0 41.0 15 37.26191578855894 40.0 38.0 41.0 32.0 41.0 16 37.21740968905289 40.0 38.0 41.0 32.0 41.0 17 37.197652927455565 40.0 38.0 41.0 32.0 41.0 18 37.153583453175656 40.0 38.0 41.0 32.0 41.0 19 37.19406401646459 40.0 38.0 41.0 32.0 41.0 20 37.11067707643379 40.0 38.0 41.0 31.0 41.0 21 37.063120958964646 40.0 38.0 41.0 31.0 41.0 22 36.97272470037654 40.0 38.0 41.0 31.0 41.0 23 36.89012728897065 40.0 38.0 41.0 31.0 41.0 24 36.82079819754324 40.0 38.0 41.0 31.0 41.0 25 36.738109116035275 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0064878555327397 1101 2 4.0265408256372766 1101 3 2.0989815584486315 1101 4 1.1726023470496187 1101 5 0.7644732958609168 1101 6 0.36715155991903714 1101 7 0.14757096773693235 1101 8 0.06475584028525816 1101 9 0.14753886437945596 1101 10 0.237755467155516 1101 11 0.31065330873278896 1101 12 0.19465058015834558 1101 13 0.32136106707299916 1101 14 0.19679564311083908 1101 15 0.3437829932058847 1101 16 0.40808071185552564 1101 17 0.43193246678632846 1101 18 0.410910984886975 1101 19 0.3811863727269156 1101 20 0.41175509741034944 1101 21 0.23164847121761056 1101 22 0.3107768170168441 1101 23 0.37241232431075133 1101 24 0.27319843414539946 1101 25 0.20849921040799302 1102 1 1.4931224931342264 1102 2 4.310240446203785 1102 3 2.421232810713988 1102 4 1.5466789679771153 1102 5 1.0433183005586315 1102 6 0.8823190935032841 1102 7 0.6382426404006196 1102 8 0.7010142007231792 1102 9 0.7007343244157482 1102 10 0.4952529712051188 1102 11 0.7426237213782017 1102 12 0.6645860991413599 1102 13 0.7592521988953465 1102 14 0.7693859707744295 1102 15 0.8165809643306972 1102 16 0.6933057886646097 1102 17 0.707032166904547 1102 18 0.8705703589044589 1102 19 0.7944826636434996 1102 20 0.8135697100431329 1102 21 0.8048130666591362 1102 22 0.7153895294790686 1102 23 0.7942895364214877 1102 24 0.8277802268537258 1102 25 0.9108109293318023 1103 1 1.0920327454254561 1103 2 3.7928542223854578 1103 3 2.0965021537953135 1103 4 1.2935991039159447 1103 5 0.9273267467578421 1103 6 0.8266503944178751 1103 7 0.6505951175002096 1103 8 0.47350289033666115 1103 9 0.5729323115120337 1103 10 0.48851195704244077 1103 11 0.5080066799992053 1103 12 0.4493234435268647 1103 13 0.46775271889944037 1103 14 0.6097232951689264 1103 15 0.6049529826367461 1103 16 0.4119880364150248 1103 17 0.516519980721668 1103 18 0.6825668652385133 1103 19 0.6475812097739251 1103 20 0.6745828493685337 1103 21 0.8014464268851285 1103 22 0.6267181220885831 1103 23 0.7229168922066194 1103 24 0.7446346066685265 1103 25 0.7903449009493357 1104 1 1.2164020067373933 1104 2 3.869735409139256 1104 3 2.092097322957361 1104 4 1.414111553250045 1104 5 0.9473759650554712 1104 6 0.6834589255894628 1104 7 0.49487159473846987 1104 8 0.5261540608222219 1104 9 0.510885547235965 1104 10 0.4622759849465936 1104 11 0.37037467294045 1104 12 0.535269344759385 1104 13 0.4895597335408439 1104 14 0.39645089877551243 1104 15 0.5120792958140115 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-0.7829436965378918 2315 10 -0.7063439117264707 2315 11 -0.6991412445400229 2315 12 -0.7508821479830772 2315 13 -0.636275499259213 2315 14 -0.975684051107983 2315 15 -1.2597366755311725 2315 16 -1.0493965934478666 2315 17 -1.0824010258080392 2315 18 -0.955548642442217 2315 19 -0.9604285341053824 2315 20 -0.987990084943533 2315 21 -0.8747378960383259 2315 22 -1.1760045175905773 2315 23 -1.0442145270987098 2315 24 -0.9152869934704668 2315 25 -0.9162021751629581 2316 1 -0.4079939135306212 2316 2 0.07369812189349645 2316 3 0.0800992057249097 2316 4 -0.12187072942456467 2316 5 -0.46962536555428613 2316 6 -0.5877428272349405 2316 7 -0.6573258222895078 2316 8 -0.7877841015282598 2316 9 -0.7670944704853824 2316 10 -0.990193689119387 2316 11 -0.9331159901618378 2316 12 -0.8197761604851053 2316 13 -1.0572064723908738 2316 14 -1.0087798659680587 2316 15 -0.9863698347637566 2316 16 -1.009459042481133 2316 17 -1.1051245795183462 2316 18 -1.1125344490480273 2316 19 -1.016366499709072 2316 20 -1.002313166347946 2316 21 -1.0592110547458233 2316 22 -1.0529750333269803 2316 23 -1.1367093492291858 2316 24 -1.209082625885749 2316 25 -1.2343792301224994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44819.0 3 1.0 4 376.0 5 1717.0 6 2141.0 7 1960.0 8 1895.0 9 1763.0 10 1808.0 11 1677.0 12 1711.0 13 1703.0 14 1748.0 15 1774.0 16 1942.0 17 2016.0 18 2384.0 19 2910.0 20 3755.0 21 4873.0 22 5826.0 23 6929.0 24 8264.0 25 9510.0 26 11212.0 27 12785.0 28 15244.0 29 18761.0 30 24287.0 31 32381.0 32 43118.0 33 60518.0 34 88012.0 35 133852.0 36 222924.0 37 318331.0 38 792275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54536716260368 19.83158135695066 13.604426023287386 25.018625457158265 2 16.23371177258343 21.938147630227707 40.189678261770474 21.638462335418396 3 17.89654737542669 25.591855031946764 30.395103438847563 26.11649415377898 4 11.647348826463729 17.743569580786794 40.089720125349594 30.519361467399886 5 12.573163869050582 38.089881210384476 36.64912394115733 12.687830979407611 6 29.791050343869042 38.704197380141274 19.145496425658955 12.359255850330728 7 26.525777314776057 32.15214905452623 23.656980015917746 17.665093614779977 8 23.68257346060464 38.03837639002079 21.625083059471113 16.653967089903464 9 25.660316171771758 15.976138219438088 21.510892845598935 36.85265276319122 10 14.023103009749892 29.878868164476476 35.612918609580326 20.485110216193302 11 32.82727551157746 23.21876513483983 25.797397416916684 18.156561936666026 12 22.432150877330567 25.26057094047166 33.035361344466565 19.2719168377312 13 28.027259403073966 22.85192576099432 26.631012472432737 22.489802363498978 14 19.888861923630856 23.107966184859915 28.93871456261704 28.06445732889219 15 21.87280428910101 31.93436632644518 24.271805561884737 21.921023822569072 16 20.874818911807004 29.167995794832773 29.04728799566766 20.909897297692563 17 20.653221011847165 29.377035420691584 28.927428012475616 21.042315554985635 18 20.305903735438044 28.319860957664684 31.246903473144005 20.127331833753267 19 22.446577782864725 27.654551784326166 28.676233306467896 21.22263712634122 20 22.42401537523229 28.142567445478488 29.472268572709385 19.961148606579833 21 21.95316594343477 27.529408398251103 28.34275945867033 22.1746661996438 22 21.909683381545083 28.925883907096427 28.723887363081367 20.44054534827712 23 21.16457544787797 28.66021576142566 28.95859794815219 21.21661084254418 24 21.850454721039252 27.751786905581334 29.743042738955733 20.654715634423678 25 21.721247845340343 28.129138099045726 29.228392068838893 20.92122198677504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 220.0 1 220.0 2 428.5 3 637.0 4 637.0 5 637.0 6 1605.5 7 2574.0 8 2574.0 9 2574.0 10 3985.0 11 5396.0 12 5396.0 13 5396.0 14 8966.0 15 12536.0 16 12536.0 17 12536.0 18 21073.0 19 29610.0 20 29610.0 21 29610.0 22 45404.0 23 61198.0 24 61198.0 25 61198.0 26 85517.5 27 109837.0 28 109837.0 29 109837.0 30 138723.0 31 167609.0 32 167609.0 33 167609.0 34 186347.5 35 205086.0 36 205086.0 37 205086.0 38 217025.5 39 228965.0 40 228965.0 41 228965.0 42 236121.0 43 243277.0 44 243277.0 45 243277.0 46 241073.0 47 238869.0 48 238869.0 49 238869.0 50 225909.0 51 212949.0 52 212949.0 53 212949.0 54 183412.0 55 153875.0 56 153875.0 57 153875.0 58 128886.0 59 103897.0 60 103897.0 61 103897.0 62 82129.0 63 60361.0 64 60361.0 65 60361.0 66 45398.5 67 30436.0 68 30436.0 69 30436.0 70 21725.5 71 13015.0 72 13015.0 73 13015.0 74 8820.5 75 4626.0 76 4626.0 77 4626.0 78 3061.0 79 1496.0 80 1496.0 81 1496.0 82 996.0 83 496.0 84 496.0 85 496.0 86 325.5 87 155.0 88 155.0 89 155.0 90 107.0 91 59.0 92 59.0 93 59.0 94 38.0 95 17.0 96 17.0 97 17.0 98 11.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.3358909115187454 3 0.0 4 0.0 5 0.0 6 5.2988498316555413E-5 7 0.0 8 0.0 9 0.0 10 1.0597699663311083E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.7091948821588785E-4 19 0.0010067814680145527 20 5.828734814821095E-4 21 0.003762183380475434 22 0.0021725284309787717 23 0.0013247124579138852 24 5.2988498316555413E-5 25 6.888504781152203E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1887202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.3976699662054 #Duplication Level Percentage of deduplicated Percentage of total 1 86.96587399886243 67.30956014086559 2 9.506215480190708 14.715178567268664 3 2.0082699291527453 4.663062395388566 4 0.6527072221246893 2.020720726502617 5 0.2722300683161565 1.0534986491205716 6 0.14197487205053522 0.6593114574276939 7 0.08943353747022088 0.4845363191921294 8 0.05928006460762128 0.36705111008608043 9 0.04931663219638953 0.34352931803228065 >10 0.22384619295944924 3.012397979978963 >50 0.015518645130763831 0.8335717912700513 >100 0.012571459074997524 1.9363887218698563 >500 0.0015880912713703346 0.8296198665448686 >1k 0.0011738065918824097 1.7715729564520863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4426 0.23452709354907425 No Hit GTCCTACAGTGGACATTTCTAAATT 3298 0.17475606744799974 No Hit CTGTAGGACGTGGAATATGGCAAGA 2948 0.15621009303720534 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2802 0.14847377228298825 No Hit TATCAACGCAGAGTACTTTTTTTTT 2597 0.1376111301280944 No Hit CTTTAGGACGTGAAATATGGCGAGG 2552 0.1352266477038494 No Hit GGTATCAACGCAGAGTACTTTTTTT 2445 0.12955687838397797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.298849831655541E-5 5.298849831655541E-5 0.0 0.0 0.0 6 5.298849831655541E-5 5.298849831655541E-5 0.0 0.0 0.0 7 5.298849831655541E-5 5.298849831655541E-5 0.0 0.0 0.0 8 1.0597699663311081E-4 5.298849831655541E-5 0.0 0.0 0.0 9 1.0597699663311081E-4 5.298849831655541E-5 0.0 0.0 0.0 10 1.0597699663311081E-4 5.298849831655541E-5 0.0 0.0 0.0 11 1.0597699663311081E-4 5.298849831655541E-5 0.0 0.0 0.0 12 1.0597699663311081E-4 5.298849831655541E-5 0.0 5.298849831655541E-5 0.0 13 1.0597699663311081E-4 5.298849831655541E-5 0.0 5.298849831655541E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACACG 50 8.9821966E-5 15.146376 4 CGTTAGG 60 1.928232E-5 14.689722 1 CGGTAGG 85 1.8642822E-7 13.825621 1 GACGTAA 50 0.0015402265 13.253078 7 GGTATCA 875 0.0 12.870995 1 TAGGACC 650 0.0 12.670526 4 GGACGTA 60 4.216579E-4 12.62198 6 CTAGGAC 310 0.0 11.604078 3 CCAACGA 175 1.8189894E-12 11.360383 19 GTCTTAA 165 4.5474735E-11 11.276958 1 CCGTGCT 60 0.006033054 11.044232 9 TCCAACG 210 0.0 10.819412 18 TATGTCG 105 3.6145102E-6 10.819412 16 GCCGGTT 125 2.352208E-7 10.602463 11 AATGTCC 510 0.0 10.580189 8 GTCCTAA 1020 0.0 10.561238 1 GTAGGAC 1990 0.0 10.46546 3 AGGACCT 1275 0.0 10.320325 5 GTATCAA 1895 0.0 10.284098 1 CTGTAGG 2025 0.0 10.204218 1 >>END_MODULE