FastQCFastQC Report
Thu 26 May 2016
SRR1512056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54950.291171798249472No Hit
TATCAACGCAGAGTACTTTTTTTTT35070.18583066359615982No Hit
GGTATCAACGCAGAGTACTTTTTTT34320.18185652622241816No Hit
GTCCTACAGTGGACATTTCTAAATT33350.1767166418857123No Hit
CTGTAGGACGTGGAATATGGCAAGA32000.1695631946129773No Hit
GTCCTAAAGTGTGTATTTCTCATTT28880.153030783138212No Hit
CTTTAGGACGTGAAATATGGCGAGG28690.15202400167019747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC250.00601807319.0079043
CCAACGA1205.2750693E-1113.45287219
GGTATCA11200.012.69955351
TCCAACG1800.012.66052118
GGCGAGG7800.012.17454419
CAGTCCG600.00589887911.0776629
GGACGTG17600.010.9038956
AGGACGT18550.010.8616585
AGAACCG700.001486492910.8616585
GACGTGG8500.010.8356287
TGTAGGA19300.010.6365472
TAGGACG19950.010.385274
GTATCAA23350.010.3022941
GTAGGAC18950.010.1810143
CCAACGT750.002656951310.12922119
TGGCGAG19800.010.11882518
TTAGGAC12050.010.0166133
GGACCGT951.6435054E-410.0004466
GGACCTG11500.09.9960996
GACGTGA9900.09.9746917
Produced by FastQC (version 0.11.5)