FastQCFastQC Report
Thu 26 May 2016
SRR1512055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072315
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45040.21734147559613282No Hit
GGTATCAACGCAGAGTACTTTTTTT28200.13607969830841354No Hit
TATCAACGCAGAGTACTTTTTTTTT27880.13453553151909822No Hit
GGTATCAACGCAGAGTACATGGGGG24010.11586076441081593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.014.0791211
TAGACGT701.12856906E-412.1703444
ACGGTGC802.9751503E-511.8322788
GTGGTAT4100.010.7562871
ACTAGAC1106.31287E-610.3263523
GTATCAA29500.010.0991611
CTTATGC1151.0715858E-59.877383
GTCTAAG1802.4465407E-99.8001731
GATATAC1604.1043677E-89.8001721
TATACTG3150.09.6160755
GTCTTAG2251.4551915E-119.5823911
GGCGTGC1002.857384E-49.4658238
TAACCTA800.0046527829.4658235
GCTCTAG1053.2906962E-49.3334981
TGGTATC4100.09.3221152
TCTACAC3050.09.3106453
CTTATAC2151.1459633E-109.24568753
ATAGGAC1857.1249815E-99.209993
GTATTAT2353.45608E-119.1746291
TTATACG850.00765957178.909014
Produced by FastQC (version 0.11.5)